The authors declare that they have no competing interests.
TT, BA conceived the study, TT and BO carried out all experiments. TT and BA drafted the paper. All authors revised and agreed on the final draft. All authors read and approved the final manuscript.
The aim of the present study was to evaluate the time-dependent effectiveness of the intracanal medicaments used in pulp revascularization on the dislocation resistance of mineral trioxide aggregate (MTA).
One hundred ninety-two extracted human maxillary incisor teeth were sectioned apically 12 mm below and coronally 2 mm above the cemento-enamel junction. Roots were enlarged to size 40 (Protaper F4). Next, Peeso reamers from #1 to #5 were used sequentially. Sodium hypochlorite (2.5 %), EDTA (17 %), and distilled water were used in final irrigation. The specimens were randomly divided into four groups (n = 48): Group 1, in which triple antibiotic paste (TAP) (ciprofloxacin + metronidazole + minocycline) was prepared and delivered into the canals using a lentulo spiral; Group 2, in which double antibiotic paste (DAP) (ciprofloxacin + metronidazole) was placed into the canals; Group 3, in which calcium hydroxide paste (CH) (calcium hydroxide + distilled water) was introduced into the roots; and Group 4 (control), in which no medicament was applied into the root canals. Then, the samples were kept in saline solution for 2, 4, and 12 weeks, after which time 16 roots were selected randomly from each group, representing the samples of each time point. After removal of the medicaments, MTA was placed into the coronal third of the roots, and the samples were incubated for 7 days. A push-out test was used to measure the dislocation resistance (DR) of MTA. The data were analyzed using a two-way ANOVA followed by Tukey’s pairwise comparisons (p = 0.05).
The time factor displayed a significant effect on the DR of MTA (p < 0.05). All medicaments resulted in significantly smaller DR values after 12 weeks compared to after 1 week (p < 0.05). A significant unfavorable effect of TAP and DAP was observed as early as 2 weeks after the application, while 2 and 4 weeks after the application of CH there was no effect on the DR of MTA. No significant differences were found between the time points in the control group (p > 0.05).
The type and the intracanal duration of medicaments used for pulp revascularization should be chosen carefully to provide maximum antimicrobial effect while creating a favorable environment both for stem cell attachment and MTA adhesion.
Nakashima M, Huang GT. Pulp and dentin regeneration. In: Huang GT, Thesleff I, editors. Stem cells in craniofacial development and regeneration. Hoboken: Wiley-Blackwell; 2013.
Miller EK, Lee JY, Tawil PZ, Teixeira FB, Vann WF Jr. Emerging therapies for the management of traumatized immature permanenet incisors. Pediatr Dent. 2012;34:66–9. PubMed
Topçuoğlu HS, Arslan H, Akçay M, Saygili G, Cakici F, Topçuoğlu G. The effect of medicaments used in endodontic regeneration technique on the dislocation resistance of mineral trioxide aggregate to root Canal dentine. Int Endod J. 2014;46:688–95.
Hasheem AA, Wannes Amin SA. The Effect of Acidity on Dislodgment Resistance of Mineral Trioxide Aggregate and Bioaggregate in Furcation Perforations. An In vitro Comparative Study. 2012;38:245–9.
Kokubo T. Surface chemistry of bioactive glass-ceramics. J Non-Cryst Solids. 1990;120:138–51. CrossRef
- Time-dependent effectiveness of the intracanal medicaments used for pulp revascularization on the dislocation resistance of MTA
- BioMed Central
Neu im Fachgebiet Zahnmedizin
Mail Icon II