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Journal of Cancer Research and Clinical Oncology

Erschienen in:

17.08.2017 | Original Article – Cancer Research

TLR9 gene polymorphism −1486T/C (rs187084) is associated with uterine cervical neoplasm in Mexican female population

verfasst von: Cecilia Martínez-Campos, Margarita Bahena-Román, Kirvis Torres-Poveda, Ana I. Burguete-García, Vicente Madrid-Marina

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 12/2017

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Abstract

Purpose

The aim of this work was to evaluate the association of single nucleotide polymorphisms in TLR9 (−1486 T/C [rs187084], −1237T/C [rs5743836] and G2848A [rs352140]) with HPV infection, squamous intraepithelial lesions, and uterine cervical neoplasm in a Mexican population. Additionally, the peripheral expression of TLR9 was evaluated to evaluate the differences in the TLR9 expression associated with every genotype in the locus −1486 of the TLR9 gene. The serum concentration of TLR9 was evaluated in a randomly selected subsample.

Methods

Genotyping was performed using predesigned 5′ endonuc lease assays and the association of the polymorphisms with the diagnosis groups were assessed by performing multinomial regression models. The relative expression of TLR9 in peripheral blood mononuclear cells was evaluated by real-time polymerase chain reaction and the association of the level of TLR9 expression with the diagnosis was evaluated by performing multinomial regression models. The serum concentration of TLR9 was evaluated in a subsample of patients diagnosed with uterine cervical neoplasm by ELISA.

Results

The results showed that genotype TT in the −1486 locus of TLR9 was significantly associated with HPV infection (OR = 3.25, 95% CI 1.12–9.46), squamous intraepithelial cervical lesion (OR = 3.76, 95% CI 1.36–10.41), and uterine cervical neoplasm (OR = 5.30, 95% CI 1.81–15.55). Moreover, the highest level of TLR9 expression was significantly associated with a greater risk for developing squamous intraepithelial cervical lesion and uterine cervical neoplasm. The serum TLR9 concentration was higher in patients with uterine cervical cancer than in controls.

Conclusion

Our findings indicate that genotype TT in the −1486 locus of the TLR9 gene could comprise a risk genotype for HPV infection, squamous intraepithelial cervical lesion, and uterine cervical neoplasm in Mexican female population. Further studies with larger samples are needed to evaluate if the peripheral expression of TLR9 could be used as a biomarker of uterine cervical neoplasm progression.

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Titel: TLR9 gene polymorphism −1486T/C (rs187084) is associated with uterine cervical neoplasm in Mexican female population
verfasst von: Cecilia Martínez-Campos
Margarita Bahena-Román
Kirvis Torres-Poveda
Ana I. Burguete-García
Vicente Madrid-Marina
Publikationsdatum: 17.08.2017
Verlag: Springer Berlin Heidelberg
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 12/2017
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-017-2495-2

Springer Medizin

TLR9 gene polymorphism −1486T/C (rs187084) is associated with uterine cervical neoplasm in Mexican female population