Tolerance of the fetus by the maternal immune system: role of inflammatory mediators at the feto-maternal interface

Placental mammals have been subjected to two opposing selective pressures during evolution, as survival of the species depends on the ability to eliminate microbial pathogens while at the same time protecting fetuses from immune rejection. In this respect, it is noteworthy that placentation had to evolve in animals that already possessed a major histocompatibility complex (MHC). One could therefore speculate that the innate immune system at the feto-maternal interface underwent less stringent selective pressures to ensure quick and efficient local protection against infection, while the adaptive immune system had to remain under full control to prevent rejection of the semi-allogeneic fetus. Given the high selective pressures at work, pregnancy failures unequivocally related to immune dysregulation are therefore rare events, whether in the human species or laboratory animals. Conversely, there are many examples of abortion or fetal distress due to placental inflammation and/or infection.

A number of excellent reviews have been published recently on adaptive immune responses during pregnancy [1‐6]. The local activation of some components of the innate immune system at the feto-maternal interface is attracting a growing interest from the reproductive immunology community. This review will emphasize aspects of the innate immune system that could contribute to reproductive failure.

Apoptosis can be triggered by the Th1 cytokine, TNFα, or the Fas ligand (Fas-L). As human syncytiotrophoblasts and cytotrophoblasts in placental villi and chorionic extravillous trophoblasts produce the Fas-L, it has been proposed that trophoblast Fas-L may contribute to placental immune privilege during pregnancy by promoting apoptosis of activated, Fas-bearing maternal lymphocytes at the feto-maternal interface (Fig. 1). This view is supported by studies with isolated human peripheral blood lymphocytes co-cultured with trophoblasts [7], but the data are less clear in animal models. The lpr mutation (defect in the function of Fas) had no effect on the outcome of pregnancy; but gld mice (lacking functional Fas-L) displayed extensive leukocyte infiltrates and cell death at the decidual-placental interface, and delivered small litters [8].

Some newly-discovered co-stimulatory molecules of the B7 family, such as B7-H1, can induce T cell apoptosis (Fig. 1). However, they can also deviate immune responses towards a Th2 phenotype, and these molecules are apparently present in the placenta [9]. Thus, the roles played by the Fas-L and the B7 family molecules in immune privilege at the fetal-maternal interface needs to be re-evaluated, especially given the possibility that the B7 molecules may affect local Th2 cytokine production.

It was thought that the main function of HLA-G may be to inhibit the cytolytic activity of maternal NK cells, but this function is being reappraised [10]. HLA-G may also interact with decidual macrophages at the feto-maternal interface, perhaps altering the profile of macrophage cytokine production (Fig. 2). The leader peptides of nascent HLA-G proteins are presented efficiently by HLA-E molecules, thus enhancing cell surface expression of HLA-E, which interacts with surface receptors on NK cells, macrophages and a variety of T cell types. One function of HLA-G, expressed by extravillous trophoblast, may thus be to fine-tune innate immunity by modulating macrophage function and indirectly inhibiting the activity of maternal NK and NK-like cells via HLA-E (Fig. 1) [10]. Recent evidence suggests that soluble HLA-G1 is immunosuppressive, induces apoptosis of activated CD8⁺ T cells and down-modulates CD4⁺ T cell proliferation. Moreover, soluble HLA-G1 could also play a role during implantation [11]. Finally, HLA-G may also be expressed in peripheral tissues during viral infections and organ transplantion, where it may protect the tissues during inflammatory responses by favoring Th2-type responses [12].

Both rodents and higher primates have a hemochorial placenta, in which fetally-derived trophoblast tissue is bathed in maternal blood lacunae. The maternal decidua represents another site of direct contact between fetal trophoblasts and maternal cells (Fig. 2). During pregnancy, the major cell type found at the site of implantation within the maternal decidua are the uterine NK cells. The time-course of their appearance and recent evidence from NK-deficient mouse models suggests that they play an important role in implantation [13‐15]. Similar cell types expressing high concentrations of the cytolytic pore-forming protein, perforin, and formerly called "granulated metrial gland" cells, are found in the murine uterus [16, 17]. Finally, a type of NK cell sharing some properties with T cells (the NKT cell) is also present and appear to play an important role in some pregnancy loss (see below).

To the best of our knowledge, there are no convincing examples showing that the semi-allogeneic fetus is rejected by the maternal adaptive immune system, in the same way as it might reject an allogeneic graft. On the contrary, experimental evidence indicates that fetal alloantigens are recognized by the maternal adaptive immune system, but this recognition induces tolerance of specific maternal T or B cells, as demonstrated in antigen-receptor transgenic models [18‐21]. Instead, most of the current evidence suggests that inflammation, complement activation and/or leukocyte infiltration precede abortion. The events leading to abortion share many of the salient features of an innate immune response, such as rapid activation, little or no specificity, and no memory. Furthermore, a growing body of evidence from animal models strongly suggest that abortions are triggered when innate immune responses or their regulators are perturbed (for reviews, see [22, 23]).

Complement activation, a component of the inflammatory response, leads to necrosis, and uncontrolled complement activation in the placenta results in fetal loss [23]. Clinically, these responses could be triggered by local necrotic lesions (due to stress or ischemia) or infections of the placenta. Thus, anti-phospholipid syndrome (APS) results in recurrent fetal loss occurring in the presence of anti-phospholipid (aPL) antibodies. Some of these antibodies may target phosphatidylserine (PS) exposed on the surface of trophoblasts, as shown by both animal and in vitro models [24]. But complement activation is indispensable for aPL antibody-mediated fetal loss, as the fetuses are resistant to aPL-induced damage in mice deficient in complement C3 [25]. Likewise, fetal loss is provoked by a deficiency in the murine complement regulator, Crry, which results in complement deposition and placenta inflammation, and fetuses are rescued from lethality when Crry-deficient mice are bred to C3-deficient mice. Along similar lines, semi-allogeneic fetuses are resorbed after treatment with an indoleamine 2,-3-dioxygenase (IDO) inhibitor, which triggers extensive inflammation, complement deposition (even in the absence of antibodies) and hemorrhagic necrosis at the feto-maternal interface [26].

The enzyme IDO, which degrades tryptophan, is expressed in syncytiotrophoblasts and macrophages during gestation. In vitro, macrophages suppress T cell activity due to degradation of tryptophan by IDO, and the suppression is reversed by treatment with an IDO inhibitor. Likewise, the tryptophan concentration is significantly lower in pregnant women than in nonpregnant controls. IDO activity appears to protect the fetus by suppressing T-cell dependent inflammatory responses to fetal alloantigens, since syngeneic fetuses are not rejected after treatment with IDO inhibitor [26]. Thus, trophoblasts and antigen-presenting cells in the placenta may protect the fetus via tryptophan catabolism, thus preventing a unique type of inflammation that involves T cell-dependent, antibody-independent complement activation. However, alternative mechanisms have been proposed to account for the results obtained with the IDO inhibitor, including indirect effects of IDO inhibition on vasoconstriction and macrophage activation [27], and death of T cells due to release of by-products of the IDO reaction, such as kynurerine, 3-hydroxykynurenine and 3-hydroxyanthranilic acid [28‐30].

Xu et al. have elegantly demonstrated the important role of Crry in the outcome of murine pregnancy [31]. Triggering of resorptions by administration of IDO inhibitor to pregnant mice was reported to depend on T cell recognition of fetal alloantigens in the presence of increased concentrations of tryptophan [26]. These resorptions were subsequently shown to result from local complement activation and inflammation. More recently, a fascinating case of serendipity led to the discovery that a deficiency in the N-terminal domain of TBP (TATA binding protein, used for promoter recognition during transcription initiation by RNA polymerases) results in a placental malfunction that triggers inflammation, hemorrhages and clotting, such that over 90 % of fetuses die in midgestation [32]. Curiously, the local placental damage was avoided by rearing the mutant fetuses in RAG-deficient mothers. Hence, although the precise cellular and molecular pathways remain to be elucidated, it is remarkable that the destruction of the feto-placental units by local innate mechanisms is prevented in the absence of a functional adaptive maternal immune system.

Infections of the genital tract of pregnant mothers by Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis cause a large but undetermined fraction of miscarriages, and about 40 % of premature births [33‐36]. Pronounced hemorrhaging and necrosis has been described during infection of the placenta by some pathogens, under conditions where direct fetal damage is not observed [37, 38]. It is conceivable that stimulation of TNFα secretion by necrotic cells in the placenta may be one of the triggers for embryo loss. Elevated levels of TNFα in the environment of the embryo has been associated with early embryo loss. Stress, which can trigger abortions, also increases the levels of TNFα, and leukocytes producing TNFα are present in the uterus and placenta. In spontaneously aborting mice, an increased expression of TNFα is observed mainly in uterine and trophoblast cells, and the same cells display enhanced expression of the TNFα receptor [39].

In mice, during infection with bacteria such as Listeria monocytogenes or Chlamydia trachomatis, maternal neutrophils are recruited to the fetomaternal interface and act as the main immune effector cells against the bacteria. The macrophage growth factor, colony stimulating factor (CSF)-1, is produced in large quantities by the uterine epithelium during pregnancy, and induces trophoblasts to synthesize neutrophil chemoattractants, cytokine-induced neutrophil chemoattractant (KC) (CXCL1), and macrophage inflammatory protein (MIP)-2 (CXCL2) [40] (Fig. 3). In the absence of CSF-1, neutrophils are not recruited and bacterial infection is unrestrained, leading to fetal demise. In parallel, the macrophage migration inhibitory factor (MIF), which is expressed in the human endometrium in early pregnancy, inhibits NK cell-mediated cytolysis and could thus contribute to immune privilege at the feto-maternal interface. Conversely, MIF also stimulates macrophage phagocytosis and secretion of TNFα and IL-1β, which could lead to recruitment and maintenance of a pool of activated phagocytes in the endometrium [41].

The Toll-like receptors TLR2 and TLR4 play different roles in pathogen recognition. TLR4 is stimulated by LPS, the most proinflammatory cell wall component of Gram-negative bacteria, while TLR2 has a broader role as a pattern recognition receptor for a variety of microbes and Gram-positive ligands [42]. Consistent with the pattern recognition preferences of TLR, mutations in the TLR4 receptor predispose humans to develop septic shock with Gram-negative bacteria [43]. TLR2 and TLR4 are expressed in the placenta, mainly in the villous and intermediate trophoblasts (Fig. 2), and incubation of placenta cultures with LPS induces IL-6 and IL-8 cytokine production [44]. At the same time, urogenital infection caused by Gram-negative bacteria is a known risk factor for premature births, and TLR4 mutations are associated with an increased risk of premature birth [43]. Thus, TLR mutations are detrimental to the outcome of pregnancy when expressed in the maternal immune system.

Shifting the immune response toward the Th2 pattern (IL-4, IL-5, IL-6) may benefit the fetus, whereas development of pro-inflammatory Th1 cells (secreting IL-2, IFNγ, TNFα) may be harmful. This view is supported by studies on different mouse strains infected by Leishmania major during pregnancy, which have shown that the Th1 anti-infectious response (spontaneous in C57B1/6 mice) is associated with some increases in fetal resorption and implantation failure rates [45]. In the BALB/c strain characterized by a Th2 (non-protective) response to infection, fetal resorption and implantation failure rates were unaffected. Conversely, in C57B1/6 mice, pregnancy was associated with decreased resistance to infection, decreased IFN-γ production, and increased levels of Th2 cytokines.

The maternal decidua also has an expanded population of Vα 14 NKT lymphocytes, which as in other tissues can be stimulated with α-galactosylceramide (α-GalCer) [46]. NKT cells can lyse infected or stressed cells with a pore-forming protein (perforin) and secrete inflammatory cytokines. NKT cells can also be stimulated by glycosyl-phosphatidylinositols derived from protozoan parasites known to provoke abortion, such as Leishmania mexicana, Plasmodium falciparum, and Trypanosoma brucei. Interestingly, stimulation of pregnant mice with α-GalCer induces abortion through a mechanism requiring NKT cell-mediated perforin-dependent killing and IFNγ and TNFα secretion, and histological inspection showed that embryonic trophoblasts die selectively following α-GalCer treatment (Fig. 4) [47]. NKT cells recognize glycolipids presented by the MHC-related molecule, CD1d, which is present on human trophoblasts [48‐50]. However, direct interaction between NKT cells and CD1d in the placenta has not been demonstrated, and it is not known whether the microbial pathogens may still induce abortion in CD1d-deficient mice.

Recent research on the mechanisms leading to abortion or premature birth have focused increasingly on the role played by the innate immune system. A Th2 cytokine profile favors successful pregnancy, while production of Th1 inflammatory cytokines and complement activation result in higher rates of abortion. Approximately 10 % of all births are preterm, and the incidence of preterm birth is increasing and remains the main cause of perinatal morbidity and mortality. Given that preterm births account for 70 % of perinatal mortality and nearly half of long-term neurologic morbidity [33], clinical intervention that could decrease the risk of inflammation during pregnancy would also contribute to the well-being of the children who are born. Although one could conceivably envision the use of therapeutic agents that interfere with complement activation or modulate TLR-dependent inflammation, the main challenge will be to develop a strategy that attenuates inflammatory responses, without jeopardizing the mother's ability to ward off microbial infections.