Erschienen in:
01.05.2008 | Poster presentation
TopBP1 contains transcriptional regulatory domains and regulates gene pathways involved in breast cancer
verfasst von:
RHG Wright, ES Dornan, MM Donaldson, M MacFarlane, P Herzyk, IM Morgan
Erschienen in:
Breast Cancer Research
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Sonderheft 2/2008
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Excerpt
TopBP1 is a nuclear protein with eight BRCT domains and is involved in many aspects of nucleic acid metabolism: it is involved in the initiation of DNA replication in the Xenopus in vitro replication system by assisting loading of polymerase onto the replication complex; it is a substrate for ATM/ATR and is essential for the ATR DNA damage signalling pathway, and is also probably involved in the actual DNA repair process; it acts as a transcriptional cofactor for E2F1 where it regulates the apoptotic function of this protein. In addition, the yeast homologues of TopBP1, Dbp11 (Saccharomyces cerevisiae) and Cut5 (Schizo-saccharomyces pombe), are also involved in replication and repair processes. TopBP1 also shares functions with BRCA1; both are involved in regulating an intact G2/M checkpoint, they colocalise to sites of DNA damage, they share sequence homology (even outside the BRCT domains), they are substrates for ATM/ATR, and they can regulate expression of the c-myc gene. All of these properties of TopBP1 led to us investigating whether TopBP1 plays a role in breast cancer. …