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Molecular Imaging and Biology

23.10.2023 | Research Article

Toward a Nanoencapsulated EPR Imaging Agent for Clinical Use

verfasst von: Rhia M. Martin, Samantha Diaz, Martin Poncelet, Benoit Driesschaert, Eugene Barth, Mrignayani Kotecha, Boris Epel, Gareth R. Eaton, Joshua R. Biller

Erschienen in: Molecular Imaging and Biology

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Abstract

Purpose

Progress toward developing a novel radiocontrast agent for determining pO2 in tumors in a clinical setting is described. The imaging agent is designed for use with electron paramagnetic resonance imaging (EPRI), in which the collision of a paramagnetic probe molecule with molecular oxygen causes a spectroscopic change which can be calibrated to give the real oxygen concentration in the tumor tissue.

Procedures

The imaging agent is based on a nanoscaffold of aluminum hydroxide (boehmite) with sizes from 100 to 200 nm, paramagnetic probe molecule, and encapsulation with a gas permeable, thin (10–20 nm) polymer layer to separate the imaging agent and body environment while still allowing O2 to interact with the paramagnetic probe. A specially designed deuterated Finland trityl (dFT) is covalently attached on the surface of the nanoparticle through 1,3-dipolar addition of the alkyne on the dFT with an azide on the surface of the nanoscaffold. This click-chemistry reaction affords 100% efficiency of the trityl attachment as followed by the complete disappearance of the azide peak in the infrared spectrum. The fully encapsulated, dFT-functionalized nanoparticle is referred to as RADI-Sense.

Results

Side-by-side in vivo imaging comparisons made in a mouse model made between RADI-Sense and free paramagnetic probe (OX-071) showed oxygen sensitivity is retained and RADI-Sense can create 3D pO2 maps of solid tumors

Conclusions

A novel encapsulated nanoparticle EPR imaging agent has been described which could be used in the future to bring EPR imaging for guidance of radiotherapy into clinical reality.
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Metadaten
Titel
Toward a Nanoencapsulated EPR Imaging Agent for Clinical Use
verfasst von
Rhia M. Martin
Samantha Diaz
Martin Poncelet
Benoit Driesschaert
Eugene Barth
Mrignayani Kotecha
Boris Epel
Gareth R. Eaton
Joshua R. Biller
Publikationsdatum
23.10.2023
Verlag
Springer International Publishing
Erschienen in
Molecular Imaging and Biology
Print ISSN: 1536-1632
Elektronische ISSN: 1860-2002
DOI
https://doi.org/10.1007/s11307-023-01863-0

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