Skip to main content
Erschienen in: Immunologic Research 1-3/2013

01.12.2013 | Immunology & Microbiology in Miami

Transdisciplinary approach to restore pancreatic islet function

verfasst von: Carmen Fotino, R. Damaris Molano, Camillo Ricordi, Antonello Pileggi

Erschienen in: Immunologic Research | Ausgabe 1-3/2013

Einloggen, um Zugang zu erhalten

Abstract

The focus of our research is on islet immunobiology. We are exploring novel strategies that could be of assistance in the treatment and prevention of type 1 diabetes, as well as in the restoration of metabolic control via transplantation of insulin producing cells (i.e., islet cells). The multiple facets of diabetes and β-cell replacement encompass different complementary disciplines, such as immunology, cell biology, pharmacology, and bioengineering, among others. Through their interaction and integration, a transdisciplinary dimension is needed in order to address and overcome all aspects of the complex puzzle toward a successful clinical translation of a biological cure for diabetes.
Literatur
2.
Zurück zum Zitat LeRoith D, Taylor SI, Olefsky JM. Diabetes mellitus: a fundamental and clinical text. 3rd ed. Philadelphia, PA: Lippincott Williams & Wilkins; 2003. LeRoith D, Taylor SI, Olefsky JM. Diabetes mellitus: a fundamental and clinical text. 3rd ed. Philadelphia, PA: Lippincott Williams & Wilkins; 2003.
3.
Zurück zum Zitat Bluestone JA, Herold K, Eisenbarth G. Genetics, pathogenesis and clinical interventions in type 1 diabetes. Nature. 2010;464(7293):1293–300.PubMedCrossRef Bluestone JA, Herold K, Eisenbarth G. Genetics, pathogenesis and clinical interventions in type 1 diabetes. Nature. 2010;464(7293):1293–300.PubMedCrossRef
6.
Zurück zum Zitat Pileggi A, Alejandro R, Ricordi C. Clinical islet transplantation. Minerva Endocrinol. 2006;31(3):219–32.PubMed Pileggi A, Alejandro R, Ricordi C. Clinical islet transplantation. Minerva Endocrinol. 2006;31(3):219–32.PubMed
8.
Zurück zum Zitat Piemonti L, Pileggi A. 25 years of the Ricordi automated method for islet isolation. CellR4. 2013;1(1):8–22. Piemonti L, Pileggi A. 25 years of the Ricordi automated method for islet isolation. CellR4. 2013;1(1):8–22.
9.
Zurück zum Zitat Pileggi A, Cobianchi L, Inverardi L, Ricordi C. Overcoming the challenges now limiting islet transplantation: a sequential, integrated approach. Ann N Y Acad Sci. 2006;1079:383–98.PubMedCrossRef Pileggi A, Cobianchi L, Inverardi L, Ricordi C. Overcoming the challenges now limiting islet transplantation: a sequential, integrated approach. Ann N Y Acad Sci. 2006;1079:383–98.PubMedCrossRef
10.
Zurück zum Zitat Vendrame F, Pileggi A, Laughlin E, Allende G, Martin-Pagola A, Molano RD et al. Recurrence of type 1 diabetes after simultaneous pancreas-kidney transplantation, despite immunosuppression, associated with autoantibodies and pathogenic autoreactive CD4 T-cells. Diabetes. 2010;59(4):947–57. doi:10.2337/db09-0498. Vendrame F, Pileggi A, Laughlin E, Allende G, Martin-Pagola A, Molano RD et al. Recurrence of type 1 diabetes after simultaneous pancreas-kidney transplantation, despite immunosuppression, associated with autoantibodies and pathogenic autoreactive CD4 T-cells. Diabetes. 2010;59(4):947–57. doi:10.​2337/​db09-0498.
11.
Zurück zum Zitat Digon BJ 3rd, Rother KI, Hirshberg B, Harlan DM. Sirolimus-induced interstitial pneumonitis in an islet transplant recipient. Diabetes Care. 2003;26(11):3191.PubMedCrossRef Digon BJ 3rd, Rother KI, Hirshberg B, Harlan DM. Sirolimus-induced interstitial pneumonitis in an islet transplant recipient. Diabetes Care. 2003;26(11):3191.PubMedCrossRef
12.
Zurück zum Zitat Hirshberg B, Rother KI, Digon BJ 3rd, Lee J, Gaglia JL, Hines K, et al. Benefits and risks of solitary islet transplantation for type 1 diabetes using steroid-sparing immunosuppression: the National Institutes of Health experience. Diabetes Care. 2003;26(12):3288–95.PubMedCrossRef Hirshberg B, Rother KI, Digon BJ 3rd, Lee J, Gaglia JL, Hines K, et al. Benefits and risks of solitary islet transplantation for type 1 diabetes using steroid-sparing immunosuppression: the National Institutes of Health experience. Diabetes Care. 2003;26(12):3288–95.PubMedCrossRef
13.
Zurück zum Zitat Cure P, Pileggi A, Froud T, Norris PM, Baidal DA, Cornejo A, et al. Alterations of the female reproductive system in recipients of islet grafts. Transplantation. 2004;78(11):1576–81.PubMedCrossRef Cure P, Pileggi A, Froud T, Norris PM, Baidal DA, Cornejo A, et al. Alterations of the female reproductive system in recipients of islet grafts. Transplantation. 2004;78(11):1576–81.PubMedCrossRef
14.
Zurück zum Zitat Hafiz MM, Faradji RN, Froud T, Pileggi A, Baidal DA, Cure P, et al. Immunosuppression and procedure-related complications in 26 patients with type 1 diabetes mellitus receiving allogeneic islet cell transplantation. Transplantation. 2005;80(12):1718–28.PubMedCrossRef Hafiz MM, Faradji RN, Froud T, Pileggi A, Baidal DA, Cure P, et al. Immunosuppression and procedure-related complications in 26 patients with type 1 diabetes mellitus receiving allogeneic islet cell transplantation. Transplantation. 2005;80(12):1718–28.PubMedCrossRef
15.
Zurück zum Zitat Molinari M, Al-Saif F, Ryan EA, Lakey JR, Senior PA, Paty BW, et al. Sirolimus-induced ulceration of the small bowel in islet transplant recipients: report of two cases. Am J Transplant. 2005;5(11):2799–804.PubMedCrossRef Molinari M, Al-Saif F, Ryan EA, Lakey JR, Senior PA, Paty BW, et al. Sirolimus-induced ulceration of the small bowel in islet transplant recipients: report of two cases. Am J Transplant. 2005;5(11):2799–804.PubMedCrossRef
16.
Zurück zum Zitat Desai NM, Goss JA, Deng S, Wolf BA, Markmann E, Palanjian M, et al. Elevated portal vein drug levels of sirolimus and tacrolimus in islet transplant recipients: local immunosuppression or islet toxicity? Transplantation. 2003;76(11):1623–5.PubMedCrossRef Desai NM, Goss JA, Deng S, Wolf BA, Markmann E, Palanjian M, et al. Elevated portal vein drug levels of sirolimus and tacrolimus in islet transplant recipients: local immunosuppression or islet toxicity? Transplantation. 2003;76(11):1623–5.PubMedCrossRef
17.
Zurück zum Zitat Shapiro AM, Ricordi C, Hering BJ, Auchincloss H, Lindblad R, Robertson RP, et al. International trial of the Edmonton protocol for islet transplantation. N Engl J Med. 2006;355(13):1318–30.PubMedCrossRef Shapiro AM, Ricordi C, Hering BJ, Auchincloss H, Lindblad R, Robertson RP, et al. International trial of the Edmonton protocol for islet transplantation. N Engl J Med. 2006;355(13):1318–30.PubMedCrossRef
18.
Zurück zum Zitat Bussiere CT, Lakey JR, Shapiro AM, Korbutt GS. The impact of the mTOR inhibitor sirolimus on the proliferation and function of pancreatic islets and ductal cells. Diabetologia. 2006;49(10):2341–9.PubMedCrossRef Bussiere CT, Lakey JR, Shapiro AM, Korbutt GS. The impact of the mTOR inhibitor sirolimus on the proliferation and function of pancreatic islets and ductal cells. Diabetologia. 2006;49(10):2341–9.PubMedCrossRef
19.
Zurück zum Zitat Zahr E, Molano RD, Pileggi A, Ichii H, Jose SS, Bocca N, et al. Rapamycin impairs in vivo proliferation of islet beta-cells. Transplantation. 2007;84(12):1576–83.PubMedCrossRef Zahr E, Molano RD, Pileggi A, Ichii H, Jose SS, Bocca N, et al. Rapamycin impairs in vivo proliferation of islet beta-cells. Transplantation. 2007;84(12):1576–83.PubMedCrossRef
22.
Zurück zum Zitat Vlodavsky E, Palzur E, Soustiel JF. Hyperbaric oxygen therapy reduces neuroinflammation and expression of matrix metalloproteinase-9 in the rat model of traumatic brain injury. Neuropathol Appl Neurobiol. 2006;32(1):40–50.PubMedCrossRef Vlodavsky E, Palzur E, Soustiel JF. Hyperbaric oxygen therapy reduces neuroinflammation and expression of matrix metalloproteinase-9 in the rat model of traumatic brain injury. Neuropathol Appl Neurobiol. 2006;32(1):40–50.PubMedCrossRef
23.
Zurück zum Zitat Wilson HD, Toepfer VE, Senapati AK, Wilson JR, Fuchs PN. Hyperbaric oxygen treatment is comparable to acetylsalicylic acid treatment in an animal model of arthritis. J Pain. 2007;8(12):924–30.PubMedCrossRef Wilson HD, Toepfer VE, Senapati AK, Wilson JR, Fuchs PN. Hyperbaric oxygen treatment is comparable to acetylsalicylic acid treatment in an animal model of arthritis. J Pain. 2007;8(12):924–30.PubMedCrossRef
24.
Zurück zum Zitat Wilson HD, Wilson JR, Fuchs PN. Hyperbaric oxygen treatment decreases inflammation and mechanical hypersensitivity in an animal model of inflammatory pain. Brain Res. 2006;1098(1):126–8.PubMedCrossRef Wilson HD, Wilson JR, Fuchs PN. Hyperbaric oxygen treatment decreases inflammation and mechanical hypersensitivity in an animal model of inflammatory pain. Brain Res. 2006;1098(1):126–8.PubMedCrossRef
25.
Zurück zum Zitat Zhang Q, Chang Q, Cox RA, Gong X, Gould LJ. Hyperbaric oxygen attenuates apoptosis and decreases inflammation in an ischemic wound model. J Invest Dermatol. 2008;128(8):2102–12.PubMed Zhang Q, Chang Q, Cox RA, Gong X, Gould LJ. Hyperbaric oxygen attenuates apoptosis and decreases inflammation in an ischemic wound model. J Invest Dermatol. 2008;128(8):2102–12.PubMed
26.
Zurück zum Zitat Gallagher KA, Goldstein LJ, Thom SR, Velazquez OC. Hyperbaric oxygen and bone marrow-derived endothelial progenitor cells in diabetic wound healing. Vascular. 2006;14(6):328–37.PubMedCrossRef Gallagher KA, Goldstein LJ, Thom SR, Velazquez OC. Hyperbaric oxygen and bone marrow-derived endothelial progenitor cells in diabetic wound healing. Vascular. 2006;14(6):328–37.PubMedCrossRef
27.
Zurück zum Zitat Goldstein LJ, Gallagher KA, Bauer SM, Bauer RJ, Baireddy V, Liu ZJ, et al. Endothelial progenitor cell release into circulation is triggered by hyperoxia-induced increases in bone marrow nitric oxide. Stem Cells (Dayton, Ohio). 2006;24(10):2309–18.CrossRef Goldstein LJ, Gallagher KA, Bauer SM, Bauer RJ, Baireddy V, Liu ZJ, et al. Endothelial progenitor cell release into circulation is triggered by hyperoxia-induced increases in bone marrow nitric oxide. Stem Cells (Dayton, Ohio). 2006;24(10):2309–18.CrossRef
28.
Zurück zum Zitat Liu ZJ, Velazquez OC. Hyperoxia, endothelial progenitor cell mobilization, and diabetic wound healing. Antioxid Redox Signal. 2008;10(11):1869–82.PubMedCentralPubMedCrossRef Liu ZJ, Velazquez OC. Hyperoxia, endothelial progenitor cell mobilization, and diabetic wound healing. Antioxid Redox Signal. 2008;10(11):1869–82.PubMedCentralPubMedCrossRef
29.
Zurück zum Zitat Milovanova TN, Bhopale VM, Sorokina EM, Moore JS, Hunt TK, Hauer-Jensen M, et al. Hyperbaric oxygen stimulates vasculogenic stem cell growth and differentiation in vivo. J Appl Physiol. 2009;106(2):711–28.PubMedCentralPubMedCrossRef Milovanova TN, Bhopale VM, Sorokina EM, Moore JS, Hunt TK, Hauer-Jensen M, et al. Hyperbaric oxygen stimulates vasculogenic stem cell growth and differentiation in vivo. J Appl Physiol. 2009;106(2):711–28.PubMedCentralPubMedCrossRef
30.
Zurück zum Zitat Estrada EJ, Valacchi F, Nicora E, Brieva S, Esteve C, Echevarria L, et al. Combined treatment of intrapancreatic autologous bone marrow stem cells and hyperbaric oxygen in type 2 diabetes mellitus. Cell Transpl. 2008;17(12):1295–304.CrossRef Estrada EJ, Valacchi F, Nicora E, Brieva S, Esteve C, Echevarria L, et al. Combined treatment of intrapancreatic autologous bone marrow stem cells and hyperbaric oxygen in type 2 diabetes mellitus. Cell Transpl. 2008;17(12):1295–304.CrossRef
31.
Zurück zum Zitat Chen SY, Chen YC, Wang JK, Hsu HP, Ho PS, Chen YC, et al. Early hyperbaric oxygen therapy attenuates disease severity in lupus-prone autoimmune (NZB × NZW) F1 mice. Clin Immunol. 2003;108(2):103–10.PubMedCrossRef Chen SY, Chen YC, Wang JK, Hsu HP, Ho PS, Chen YC, et al. Early hyperbaric oxygen therapy attenuates disease severity in lupus-prone autoimmune (NZB × NZW) F1 mice. Clin Immunol. 2003;108(2):103–10.PubMedCrossRef
33.
Zurück zum Zitat Juang JH, Hsu BR, Kuo CH, Uengt SW. Beneficial effects of hyperbaric oxygen therapy on islet transplantation. Cell Transpl. 2002;11(2):95–101. Juang JH, Hsu BR, Kuo CH, Uengt SW. Beneficial effects of hyperbaric oxygen therapy on islet transplantation. Cell Transpl. 2002;11(2):95–101.
34.
Zurück zum Zitat Faleo G, Fotino C, Bocca N, Molano RD, Zahr-Akrawi E, Molina J, et al. Prevention of autoimmune diabetes and induction of beta-cell proliferation in NOD mice by hyperbaric oxygen therapy. Diabetes. 2012;61(7):1769–78. doi:10.2337/db11-0516.PubMedCentralPubMedCrossRef Faleo G, Fotino C, Bocca N, Molano RD, Zahr-Akrawi E, Molina J, et al. Prevention of autoimmune diabetes and induction of beta-cell proliferation in NOD mice by hyperbaric oxygen therapy. Diabetes. 2012;61(7):1769–78. doi:10.​2337/​db11-0516.PubMedCentralPubMedCrossRef
35.
Zurück zum Zitat Matzinger P. Introduction to the series. Danger model of immunity. Scand J Immunol. 2001;54(1–2):2–3.PubMedCrossRef Matzinger P. Introduction to the series. Danger model of immunity. Scand J Immunol. 2001;54(1–2):2–3.PubMedCrossRef
36.
Zurück zum Zitat Ribeiro MM, Klein D, Pileggi A, Molano RD, Fraker C, Ricordi C, et al. Heme oxygenase-1 fused to a TAT peptide transduces and protects pancreatic beta-cells. Biochem Biophys Res Commun. 2003;305(4):876–81.PubMedCrossRef Ribeiro MM, Klein D, Pileggi A, Molano RD, Fraker C, Ricordi C, et al. Heme oxygenase-1 fused to a TAT peptide transduces and protects pancreatic beta-cells. Biochem Biophys Res Commun. 2003;305(4):876–81.PubMedCrossRef
37.
38.
Zurück zum Zitat Fornoni A, Pileggi A, Molano RD, Sanabria NY, Tejada T, Gonzalez-Quintana J, et al. Inhibition of c-jun N terminal kinase (JNK) improves functional beta cell mass in human islets and leads to AKT and glycogen synthase kinase-3 (GSK-3) phosphorylation. Diabetologia. 2008;51(2):298–308. doi:10.1007/s00125-007-0889-4.PubMedCrossRef Fornoni A, Pileggi A, Molano RD, Sanabria NY, Tejada T, Gonzalez-Quintana J, et al. Inhibition of c-jun N terminal kinase (JNK) improves functional beta cell mass in human islets and leads to AKT and glycogen synthase kinase-3 (GSK-3) phosphorylation. Diabetologia. 2008;51(2):298–308. doi:10.​1007/​s00125-007-0889-4.PubMedCrossRef
39.
Zurück zum Zitat Bravo-Egana V, Rosero S, Klein D, Jiang Z, Vargas N, Tsinoremas N, et al. Inflammation-mediated regulation of microRNA expression in transplanted pancreatic islets. J Transpl. 2012;2012:723614. doi:10.1155/2012/723614. Bravo-Egana V, Rosero S, Klein D, Jiang Z, Vargas N, Tsinoremas N, et al. Inflammation-mediated regulation of microRNA expression in transplanted pancreatic islets. J Transpl. 2012;2012:723614. doi:10.​1155/​2012/​723614.
40.
Zurück zum Zitat Hogan AR, Doni M, Molano RD, Ribeiro MM, Szeto A, Cobianchi L, et al. Beneficial effects of ischemic preconditioning on pancreas cold preservation. Cell Transpl. 2012;21(7):1349–60. doi:10.3727/096368911X623853.CrossRef Hogan AR, Doni M, Molano RD, Ribeiro MM, Szeto A, Cobianchi L, et al. Beneficial effects of ischemic preconditioning on pancreas cold preservation. Cell Transpl. 2012;21(7):1349–60. doi:10.​3727/​096368911X623853​.CrossRef
41.
Zurück zum Zitat Pileggi A, Klein D, Fotino C, Bravo-Engaña V, Doni M, Podetta M et al. MicroRNAs in islet autoimmunity and transplantation. Immunol Res. 2013. doi:10.1007/s12026-013-8436-5. Pileggi A, Klein D, Fotino C, Bravo-Engaña V, Doni M, Podetta M et al. MicroRNAs in islet autoimmunity and transplantation. Immunol Res. 2013. doi:10.​1007/​s12026-013-8436-5.
42.
Zurück zum Zitat Cechin SR, Perez-Alvarez I, Fenjves E, Molano RD, Pileggi A, Berggren PO, et al. Anti-inflammatory properties of exenatide in human pancreatic islets. Cell Transpl. 2012;21(4):633–48. doi:10.3727/096368911X576027.CrossRef Cechin SR, Perez-Alvarez I, Fenjves E, Molano RD, Pileggi A, Berggren PO, et al. Anti-inflammatory properties of exenatide in human pancreatic islets. Cell Transpl. 2012;21(4):633–48. doi:10.​3727/​096368911X576027​.CrossRef
45.
Zurück zum Zitat Pratschke J, Tullius SG, Neuhaus P. Brain death associated ischemia/reperfusion injury. Ann Transpl. 2004;9(1):78–80. Pratschke J, Tullius SG, Neuhaus P. Brain death associated ischemia/reperfusion injury. Ann Transpl. 2004;9(1):78–80.
46.
Zurück zum Zitat Shoskes DA, Halloran PF. Delayed graft function in renal transplantation: etiology, management and long-term significance. J Urol. 1996;155(6):1831–40.PubMedCrossRef Shoskes DA, Halloran PF. Delayed graft function in renal transplantation: etiology, management and long-term significance. J Urol. 1996;155(6):1831–40.PubMedCrossRef
47.
Zurück zum Zitat Pileggi A, Molano RD, Berney T, Ichii H, San Jose S, Zahr E, et al. Prolonged allogeneic islet graft survival by protoporphyrins. Cell Transpl. 2005;14(2–3):85–96.CrossRef Pileggi A, Molano RD, Berney T, Ichii H, San Jose S, Zahr E, et al. Prolonged allogeneic islet graft survival by protoporphyrins. Cell Transpl. 2005;14(2–3):85–96.CrossRef
48.
Zurück zum Zitat Pileggi A, Molano RD, Berney T, Cattan P, Vizzardelli C, Oliver R, et al. Heme oxygenase-1 induction in islet cells results in protection from apoptosis and improved in vivo function after transplantation. Diabetes. 2001;50(9):1983–91.PubMedCrossRef Pileggi A, Molano RD, Berney T, Cattan P, Vizzardelli C, Oliver R, et al. Heme oxygenase-1 induction in islet cells results in protection from apoptosis and improved in vivo function after transplantation. Diabetes. 2001;50(9):1983–91.PubMedCrossRef
50.
51.
Zurück zum Zitat Alejandro R, Barton FB, Hering BJ, Wease S. 2008 Update from the collaborative Islet transplant registry. Transplantation. 2008;86(12):1783–8.PubMedCrossRef Alejandro R, Barton FB, Hering BJ, Wease S. 2008 Update from the collaborative Islet transplant registry. Transplantation. 2008;86(12):1783–8.PubMedCrossRef
52.
Zurück zum Zitat Turgeon NA, Avila JG, Cano JA, Hutchinson JJ, Badell IR, Page AJ, et al. Experience with a novel efalizumab-based immunosuppressive regimen to facilitate single donor islet cell transplantation. Am J Transpl. 2010;10(9):2082–91. doi:10.1111/j.1600-6143.2010.03212.x.CrossRef Turgeon NA, Avila JG, Cano JA, Hutchinson JJ, Badell IR, Page AJ, et al. Experience with a novel efalizumab-based immunosuppressive regimen to facilitate single donor islet cell transplantation. Am J Transpl. 2010;10(9):2082–91. doi:10.​1111/​j.​1600-6143.​2010.​03212.​x.CrossRef
55.
Zurück zum Zitat Chowdhury SA, Nagata M, Yamada K, Nakayama M, Chakrabarty S, Jin Z, et al. Tolerance mechanisms in murine autoimmune diabetes induced by anti-ICAM-1/LFA-1 mAb and anti-CD8 mAb. Kobe J Med Sci. 2002;48(5–6):167–75.PubMed Chowdhury SA, Nagata M, Yamada K, Nakayama M, Chakrabarty S, Jin Z, et al. Tolerance mechanisms in murine autoimmune diabetes induced by anti-ICAM-1/LFA-1 mAb and anti-CD8 mAb. Kobe J Med Sci. 2002;48(5–6):167–75.PubMed
56.
Zurück zum Zitat Hasegawa Y, Yokono K, Taki T, Amano K, Tominaga Y, Yoneda R, et al. Prevention of autoimmune insulin-dependent diabetes in non-obese diabetic mice by anti-LFA-1 and anti-ICAM-1 mAb. Int Immunol. 1994;6(6):831–8.PubMedCrossRef Hasegawa Y, Yokono K, Taki T, Amano K, Tominaga Y, Yoneda R, et al. Prevention of autoimmune insulin-dependent diabetes in non-obese diabetic mice by anti-LFA-1 and anti-ICAM-1 mAb. Int Immunol. 1994;6(6):831–8.PubMedCrossRef
59.
Zurück zum Zitat Molano RD, Berney T, Li H, Cattan P, Pileggi A, Vizzardelli C, et al. Prolonged islet graft survival in NOD mice by blockade of the CD40-CD154 pathway of T-cell costimulation. Diabetes. 2001;50(2):270–6.PubMedCrossRef Molano RD, Berney T, Li H, Cattan P, Pileggi A, Vizzardelli C, et al. Prolonged islet graft survival in NOD mice by blockade of the CD40-CD154 pathway of T-cell costimulation. Diabetes. 2001;50(2):270–6.PubMedCrossRef
60.
Zurück zum Zitat Arefanian H, Tredget EB, Rajotte RV, Korbutt GS, Gill RG, Rayat GR. Combination of anti-CD4 with anti-LFA-1 and anti-CD154 monoclonal antibodies promotes long-term survival and function of neonatal porcine islet xenografts in spontaneously diabetic NOD mice. Cell Transpl. 2007;16(8):787–98.CrossRef Arefanian H, Tredget EB, Rajotte RV, Korbutt GS, Gill RG, Rayat GR. Combination of anti-CD4 with anti-LFA-1 and anti-CD154 monoclonal antibodies promotes long-term survival and function of neonatal porcine islet xenografts in spontaneously diabetic NOD mice. Cell Transpl. 2007;16(8):787–98.CrossRef
61.
Zurück zum Zitat Molano RD, Pileggi A, Berney T, Poggioli R, Zahr E, Oliver R, et al. Prolonged islet allograft survival in diabetic NOD mice by targeting CD45RB and CD154. Diabetes. 2003;52(4):957–64.PubMedCrossRef Molano RD, Pileggi A, Berney T, Poggioli R, Zahr E, Oliver R, et al. Prolonged islet allograft survival in diabetic NOD mice by targeting CD45RB and CD154. Diabetes. 2003;52(4):957–64.PubMedCrossRef
64.
Zurück zum Zitat Chalmin F, Mignot G, Bruchard M, Chevriaux A, Vegran F, Hichami A, et al. Stat3 and Gfi-1 transcription factors control Th17 cell immunosuppressive activity via the regulation of ectonucleotidase expression. Immunity. 2012;36(3):362–73. doi:10.1016/j.immuni.2011.12.019.PubMedCrossRef Chalmin F, Mignot G, Bruchard M, Chevriaux A, Vegran F, Hichami A, et al. Stat3 and Gfi-1 transcription factors control Th17 cell immunosuppressive activity via the regulation of ectonucleotidase expression. Immunity. 2012;36(3):362–73. doi:10.​1016/​j.​immuni.​2011.​12.​019.PubMedCrossRef
65.
Zurück zum Zitat Placido R, Auricchio G, Falzoni S, Battistini L, Colizzi V, Brunetti E, et al. P2X(7) purinergic receptors and extracellular ATP mediate apoptosis of human monocytes/macrophages infected with Mycobacterium tuberculosis reducing the intracellular bacterial viability. Cell Immunol. 2006;244(1):10–8. doi:10.1016/j.cellimm.2007.02.001.PubMedCrossRef Placido R, Auricchio G, Falzoni S, Battistini L, Colizzi V, Brunetti E, et al. P2X(7) purinergic receptors and extracellular ATP mediate apoptosis of human monocytes/macrophages infected with Mycobacterium tuberculosis reducing the intracellular bacterial viability. Cell Immunol. 2006;244(1):10–8. doi:10.​1016/​j.​cellimm.​2007.​02.​001.PubMedCrossRef
67.
Zurück zum Zitat Lappas CM, Rieger JM, Linden J. A2A adenosine receptor induction inhibits IFN-gamma production in murine CD4+ T cells. J Immunol. 2005;174(2):1073–80.PubMedCrossRef Lappas CM, Rieger JM, Linden J. A2A adenosine receptor induction inhibits IFN-gamma production in murine CD4+ T cells. J Immunol. 2005;174(2):1073–80.PubMedCrossRef
69.
Zurück zum Zitat Borsellino G, Kleinewietfeld M, Di Mitri D, Sternjak A, Diamantini A, Giometto R, et al. Expression of ectonucleotidase CD39 by Foxp3 + Treg cells: hydrolysis of extracellular ATP and immune suppression. Blood. 2007;110(4):1225–32. doi:10.1182/blood-2006-12-064527.PubMedCrossRef Borsellino G, Kleinewietfeld M, Di Mitri D, Sternjak A, Diamantini A, Giometto R, et al. Expression of ectonucleotidase CD39 by Foxp3 + Treg cells: hydrolysis of extracellular ATP and immune suppression. Blood. 2007;110(4):1225–32. doi:10.​1182/​blood-2006-12-064527.PubMedCrossRef
70.
Zurück zum Zitat Romio M, Reinbeck B, Bongardt S, Huls S, Burghoff S, Schrader J. Extracellular purine metabolism and signaling of CD73-derived adenosine in murine Treg and Teff cells. Am J Physiol Cell Physiol. 2011;301(2):C530–9. doi:10.1152/ajpcell.0 0385.2010.PubMedCrossRef Romio M, Reinbeck B, Bongardt S, Huls S, Burghoff S, Schrader J. Extracellular purine metabolism and signaling of CD73-derived adenosine in murine Treg and Teff cells. Am J Physiol Cell Physiol. 2011;301(2):C530–9. doi:10.​1152/​ajpcell.​0 0385.2010.PubMedCrossRef
71.
Zurück zum Zitat Ghaemi Oskouie F, Shameli A, Yang A, Desrosiers MD, Mucsi AD, Blackburn MR, et al. High levels of adenosine deaminase on dendritic cells promote autoreactive T cell activation and diabetes in nonobese diabetic mice. J Immunol. 2011;186(12):6798–806. doi:10.4049/jimmunol.1004222.PubMedCrossRef Ghaemi Oskouie F, Shameli A, Yang A, Desrosiers MD, Mucsi AD, Blackburn MR, et al. High levels of adenosine deaminase on dendritic cells promote autoreactive T cell activation and diabetes in nonobese diabetic mice. J Immunol. 2011;186(12):6798–806. doi:10.​4049/​jimmunol.​1004222.PubMedCrossRef
74.
Zurück zum Zitat Jacques-Silva MC, Correa-Medina M, Cabrera O, Rodriguez-Diaz R, Makeeva N, Fachado A, et al. ATP-gated P2X3 receptors constitute a positive autocrine signal for insulin release in the human pancreatic beta cell. Proc Natl Acad Sci USA. 2010;107(14):6465–70. doi:10.1073/pnas.0908935107.PubMedCentralPubMedCrossRef Jacques-Silva MC, Correa-Medina M, Cabrera O, Rodriguez-Diaz R, Makeeva N, Fachado A, et al. ATP-gated P2X3 receptors constitute a positive autocrine signal for insulin release in the human pancreatic beta cell. Proc Natl Acad Sci USA. 2010;107(14):6465–70. doi:10.​1073/​pnas.​0908935107.PubMedCentralPubMedCrossRef
75.
Zurück zum Zitat Kittel A, Garrido M, Varga G. Localization of NTPDase1/CD39 in normal and transformed human pancreas. J Histochem Cytochem. 2002;50(4):549–56.PubMedCrossRef Kittel A, Garrido M, Varga G. Localization of NTPDase1/CD39 in normal and transformed human pancreas. J Histochem Cytochem. 2002;50(4):549–56.PubMedCrossRef
76.
77.
Zurück zum Zitat Kemp CB, Knight MJ, Scharp DW, Ballinger WF, Lacy PE. Effect of transplantation site on the results of pancreatic islet isografts in diabetic rats. Diabetologia. 1973;9(6):486–91.PubMedCrossRef Kemp CB, Knight MJ, Scharp DW, Ballinger WF, Lacy PE. Effect of transplantation site on the results of pancreatic islet isografts in diabetic rats. Diabetologia. 1973;9(6):486–91.PubMedCrossRef
78.
Zurück zum Zitat Scharp DW, Marchetti P, Swanson C, Newton M, McCullough CS, Olack B. The effect of transplantation site and islet mass on long-term survival and metabolic and hormonal function of canine purified islet autografts. Cell Transpl. 1992;1(2–3):245–54. Scharp DW, Marchetti P, Swanson C, Newton M, McCullough CS, Olack B. The effect of transplantation site and islet mass on long-term survival and metabolic and hormonal function of canine purified islet autografts. Cell Transpl. 1992;1(2–3):245–54.
79.
Zurück zum Zitat Ricordi C, Scharp DW, Lacy PE. Reversal of diabetes in nude mice after transplantation of fresh and 7-day-cultured (24 °C) human pancreatic islets. Transplantation. 1988;45(5):994–6.PubMedCrossRef Ricordi C, Scharp DW, Lacy PE. Reversal of diabetes in nude mice after transplantation of fresh and 7-day-cultured (24 °C) human pancreatic islets. Transplantation. 1988;45(5):994–6.PubMedCrossRef
80.
Zurück zum Zitat Lanza RP, Borland KM, Lodge P, Carretta M, Sullivan SJ, Muller TE, et al. Treatment of severely diabetic pancreatectomized dogs using a diffusion-based hybrid pancreas. Diabetes. 1992;41(7):886–9.PubMedCrossRef Lanza RP, Borland KM, Lodge P, Carretta M, Sullivan SJ, Muller TE, et al. Treatment of severely diabetic pancreatectomized dogs using a diffusion-based hybrid pancreas. Diabetes. 1992;41(7):886–9.PubMedCrossRef
81.
Zurück zum Zitat Petruzzo P, Pibiri L, De Giudici MA, Basta G, Calafiore R, Falorni A, et al. Xenotransplantation of microencapsulated pancreatic islets contained in a vascular prosthesis: preliminary results. Transpl Int. 1991;4(4):200–4.PubMedCrossRef Petruzzo P, Pibiri L, De Giudici MA, Basta G, Calafiore R, Falorni A, et al. Xenotransplantation of microencapsulated pancreatic islets contained in a vascular prosthesis: preliminary results. Transpl Int. 1991;4(4):200–4.PubMedCrossRef
82.
Zurück zum Zitat Pileggi A, Molano RD, Ricordi C, Zahr E, Collins J, Valdes R, et al. Reversal of diabetes by pancreatic islet transplantation into a subcutaneous, neovascularized device. Transplantation. 2006;81(9):1318–24.PubMedCrossRef Pileggi A, Molano RD, Ricordi C, Zahr E, Collins J, Valdes R, et al. Reversal of diabetes by pancreatic islet transplantation into a subcutaneous, neovascularized device. Transplantation. 2006;81(9):1318–24.PubMedCrossRef
83.
Zurück zum Zitat Yasunami Y, Lacy PE, Finke EH. A new site for islet transplantation—a peritoneal-omental pouch. Transplantation. 1983;36(2):181–2.PubMedCrossRef Yasunami Y, Lacy PE, Finke EH. A new site for islet transplantation—a peritoneal-omental pouch. Transplantation. 1983;36(2):181–2.PubMedCrossRef
84.
Zurück zum Zitat Guan J, Zucker PF, Atkison P, Behme MT, Dupre J, Stiller CR. Liver-omental pouch and intrahepatic islet transplants produce portal insulin delivery and prevent hyperinsulinemia in rats. Transpl Proc. 1995;27(6):3236. Guan J, Zucker PF, Atkison P, Behme MT, Dupre J, Stiller CR. Liver-omental pouch and intrahepatic islet transplants produce portal insulin delivery and prevent hyperinsulinemia in rats. Transpl Proc. 1995;27(6):3236.
85.
Zurück zum Zitat Guan J, Behme MT, Zucker P, Atkison P, Hramiak I, Zhong R, et al. Glucose turnover and insulin sensitivity in rats with pancreatic islet transplants. Diabetes. 1998;47(7):1020–6.PubMedCrossRef Guan J, Behme MT, Zucker P, Atkison P, Hramiak I, Zhong R, et al. Glucose turnover and insulin sensitivity in rats with pancreatic islet transplants. Diabetes. 1998;47(7):1020–6.PubMedCrossRef
86.
Zurück zum Zitat Kin T, Korbutt GS, Rajotte RV. Survival and metabolic function of syngeneic rat islet grafts transplanted in the omental pouch. Am J Transpl. 2003;3(3):281–5.CrossRef Kin T, Korbutt GS, Rajotte RV. Survival and metabolic function of syngeneic rat islet grafts transplanted in the omental pouch. Am J Transpl. 2003;3(3):281–5.CrossRef
87.
Zurück zum Zitat Kenyon NS, Berman DM, O’Neil JJ, Kenyon NM, Zimmerman M, Pileggi A, et al. Survival and function of allogeneic islets implanted in an omental pouch in cynomolgus monkeys (Macaca fascicularis). Xenotransplantation. 2007;14(5):406. Kenyon NS, Berman DM, O’Neil JJ, Kenyon NM, Zimmerman M, Pileggi A, et al. Survival and function of allogeneic islets implanted in an omental pouch in cynomolgus monkeys (Macaca fascicularis). Xenotransplantation. 2007;14(5):406.
88.
Zurück zum Zitat Ao Z, Matayoshi K, Yakimets WJ, Katyal D, Rajotte RV, Warnock GL. Development of an omental pouch site for islet transplantation. Transpl Proc. 1992;24(6):2789. Ao Z, Matayoshi K, Yakimets WJ, Katyal D, Rajotte RV, Warnock GL. Development of an omental pouch site for islet transplantation. Transpl Proc. 1992;24(6):2789.
89.
Zurück zum Zitat Ao Z, Matayoshi K, Lakey JR, Rajotte RV, Warnock GL. Survival and function of purified islets in the omental pouch site of outbred dogs. Transplantation. 1993;56(3):524–9.PubMedCrossRef Ao Z, Matayoshi K, Lakey JR, Rajotte RV, Warnock GL. Survival and function of purified islets in the omental pouch site of outbred dogs. Transplantation. 1993;56(3):524–9.PubMedCrossRef
90.
Zurück zum Zitat Simeonovic CJ, Dhall DP, Wilson JD, Lafferty KJ. A comparative study of transplant sites for endocrine tissue transplantation in the pig. Aust J Exp Biol Med Sci. 1986;64(Pt 1):37–41.PubMedCrossRef Simeonovic CJ, Dhall DP, Wilson JD, Lafferty KJ. A comparative study of transplant sites for endocrine tissue transplantation in the pig. Aust J Exp Biol Med Sci. 1986;64(Pt 1):37–41.PubMedCrossRef
91.
Zurück zum Zitat Weber CJ, Hardy MA, Pi-Sunyer F, Zimmerman E, Reemtsma K. Tissue culture preservation and intramuscular transplantation of pancreatic islets. Surgery. 1978;84(1):166–74.PubMed Weber CJ, Hardy MA, Pi-Sunyer F, Zimmerman E, Reemtsma K. Tissue culture preservation and intramuscular transplantation of pancreatic islets. Surgery. 1978;84(1):166–74.PubMed
92.
Zurück zum Zitat Rafael E, Tibell A, Ryden M, Lundgren T, Savendahl L, Borgstrom B et al. Intramuscular autotransplantation of pancreatic islets in a 7-year-old child: a 2-year follow-up. Am J Transpl. 2008;8(2):458–62. Rafael E, Tibell A, Ryden M, Lundgren T, Savendahl L, Borgstrom B et al. Intramuscular autotransplantation of pancreatic islets in a 7-year-old child: a 2-year follow-up. Am J Transpl. 2008;8(2):458–62.
93.
Zurück zum Zitat Speier S, Nyqvist D, Cabrera O, Yu J, Molano RD, Pileggi A, et al. Noninvasive in vivo imaging of pancreatic islet cell biology. Nat Med. 2008;14(5):574–8.PubMedCentralPubMedCrossRef Speier S, Nyqvist D, Cabrera O, Yu J, Molano RD, Pileggi A, et al. Noninvasive in vivo imaging of pancreatic islet cell biology. Nat Med. 2008;14(5):574–8.PubMedCentralPubMedCrossRef
95.
Zurück zum Zitat Perez VL, Caicedo A, Berman DM, Arrieta E, Abdulreda MH, Rodriguez-Diaz R, et al. The anterior chamber of the eye as a clinical transplantation site for the treatment of diabetes: a study in a baboon model of diabetes. Diabetologia. 2011;54(5):1121–6. doi:10.1007/s00125-011-2091-y.PubMedCentralPubMedCrossRef Perez VL, Caicedo A, Berman DM, Arrieta E, Abdulreda MH, Rodriguez-Diaz R, et al. The anterior chamber of the eye as a clinical transplantation site for the treatment of diabetes: a study in a baboon model of diabetes. Diabetologia. 2011;54(5):1121–6. doi:10.​1007/​s00125-011-2091-y.PubMedCentralPubMedCrossRef
96.
Zurück zum Zitat Kakabadze Z, Gupta S, Pileggi A, Molano RD, Ricordi C, Shatirishvili G, et al. Correction of diabetes mellitus by transplanting minimal mass of syngeneic islets into vascularized small intestinal segment. Am J Transpl. 2013. doi:10.1111/ajt.12412. Kakabadze Z, Gupta S, Pileggi A, Molano RD, Ricordi C, Shatirishvili G, et al. Correction of diabetes mellitus by transplanting minimal mass of syngeneic islets into vascularized small intestinal segment. Am J Transpl. 2013. doi:10.​1111/​ajt.​12412.
97.
Zurück zum Zitat Joseph B, Berishvili E, Benten D, Kumaran V, Liponava E, Bhargava K, et al. Isolated small intestinal segments support auxiliary livers with maintenance of hepatic functions. Nat Med. 2004;10(7):749–53.PubMedCrossRef Joseph B, Berishvili E, Benten D, Kumaran V, Liponava E, Bhargava K, et al. Isolated small intestinal segments support auxiliary livers with maintenance of hepatic functions. Nat Med. 2004;10(7):749–53.PubMedCrossRef
101.
Zurück zum Zitat Wang Y, Lanzoni G, Carpino G, Cui CB, Dominguez-Bendala J, Wauthier E et al. Biliary tree stem cells, precursors to pancreatic committed progenitors: evidence for possible life-long pancreatic organogenesis. Stem cells (Dayton, Ohio). 2013. doi:10.1002/stem.1460. Wang Y, Lanzoni G, Carpino G, Cui CB, Dominguez-Bendala J, Wauthier E et al. Biliary tree stem cells, precursors to pancreatic committed progenitors: evidence for possible life-long pancreatic organogenesis. Stem cells (Dayton, Ohio). 2013. doi:10.​1002/​stem.​1460.
103.
Zurück zum Zitat Dardenne S, Sterkers A, Leroy C, Da Mata L, Zerbib P, Pruvot FR, et al. Laparoscopic spleen-preserving distal pancreatectomy followed by intramuscular autologous islet transplantation for traumatic pancreatic transection in a young adult. JOP. 2012;13(3):285–8.PubMed Dardenne S, Sterkers A, Leroy C, Da Mata L, Zerbib P, Pruvot FR, et al. Laparoscopic spleen-preserving distal pancreatectomy followed by intramuscular autologous islet transplantation for traumatic pancreatic transection in a young adult. JOP. 2012;13(3):285–8.PubMed
105.
Zurück zum Zitat Basta G, Montanucci P, Luca G, Boselli C, Noya G, Barbaro B, et al. Long-term metabolic and immunological follow-up of nonimmunosuppressed patients with type 1 diabetes treated with microencapsulated islet allografts: four cases. Diabetes Care. 2011;34(11):2406–9. doi:10.2337/dc11-0731.PubMedCentralPubMedCrossRef Basta G, Montanucci P, Luca G, Boselli C, Noya G, Barbaro B, et al. Long-term metabolic and immunological follow-up of nonimmunosuppressed patients with type 1 diabetes treated with microencapsulated islet allografts: four cases. Diabetes Care. 2011;34(11):2406–9. doi:10.​2337/​dc11-0731.PubMedCentralPubMedCrossRef
106.
Zurück zum Zitat Maffi P, Balzano G, Ponzoni M, Nano R, Sordi V, Melzi R, et al. Autologous pancreatic islet transplantation in human bone marrow. Diabetes. 2013. doi:10.2337/db13-0465. Maffi P, Balzano G, Ponzoni M, Nano R, Sordi V, Melzi R, et al. Autologous pancreatic islet transplantation in human bone marrow. Diabetes. 2013. doi:10.​2337/​db13-0465.
108.
Zurück zum Zitat Pedraza E, Brady AC, Fraker CA, Molano RD, Sukert S, Berman DM, et al. Macroporous three-dimensional PDMS scaffolds for extrahepatic islet transplantation. Cell Transpl. 2013;22(7):1123–35. doi:10.3727/096368912X657440.CrossRef Pedraza E, Brady AC, Fraker CA, Molano RD, Sukert S, Berman DM, et al. Macroporous three-dimensional PDMS scaffolds for extrahepatic islet transplantation. Cell Transpl. 2013;22(7):1123–35. doi:10.​3727/​096368912X657440​.CrossRef
109.
Zurück zum Zitat Giuliani M, Moritz W, Bodmer E, Dindo D, Kugelmeier P, Lehmann R, et al. Central necrosis in isolated hypoxic human pancreatic islets: evidence for postisolation ischemia. Cell Transpl. 2005;14(1):67–76.CrossRef Giuliani M, Moritz W, Bodmer E, Dindo D, Kugelmeier P, Lehmann R, et al. Central necrosis in isolated hypoxic human pancreatic islets: evidence for postisolation ischemia. Cell Transpl. 2005;14(1):67–76.CrossRef
110.
Zurück zum Zitat Moritz W, Meier F, Stroka DM, Giuliani M, Kugelmeier P, Nett PC, et al. Apoptosis in hypoxic human pancreatic islets correlates with HIF-1alpha expression. Faseb J. 2002;16(7):745–7.PubMed Moritz W, Meier F, Stroka DM, Giuliani M, Kugelmeier P, Nett PC, et al. Apoptosis in hypoxic human pancreatic islets correlates with HIF-1alpha expression. Faseb J. 2002;16(7):745–7.PubMed
111.
Zurück zum Zitat Emamaullee JA, Rajotte RV, Liston P, Korneluk RG, Lakey JR, Shapiro AM, et al. XIAP overexpression in human islets prevents early posttransplant apoptosis and reduces the islet mass needed to treat diabetes. Diabetes. 2005;54(9):2541–8.PubMedCrossRef Emamaullee JA, Rajotte RV, Liston P, Korneluk RG, Lakey JR, Shapiro AM, et al. XIAP overexpression in human islets prevents early posttransplant apoptosis and reduces the islet mass needed to treat diabetes. Diabetes. 2005;54(9):2541–8.PubMedCrossRef
112.
Zurück zum Zitat Li X, Chen H, Epstein PN. Metallothionein protects islets from hypoxia and extends islet graft survival by scavenging most kinds of reactive oxygen species. J Biol Chem. 2004;279(1):765–71.PubMedCrossRef Li X, Chen H, Epstein PN. Metallothionein protects islets from hypoxia and extends islet graft survival by scavenging most kinds of reactive oxygen species. J Biol Chem. 2004;279(1):765–71.PubMedCrossRef
113.
Zurück zum Zitat Bocca N, Ricordi C, Kenyon NS, Latta P, Buchwald P. 3-D multiphysics FEM modeling to optimize local drug delivery in a biohybrid device designed for cell transplant. In: Proceedings of the Comsol Conference. 2007. p. 101–7. Bocca N, Ricordi C, Kenyon NS, Latta P, Buchwald P. 3-D multiphysics FEM modeling to optimize local drug delivery in a biohybrid device designed for cell transplant. In: Proceedings of the Comsol Conference. 2007. p. 101–7.
114.
Zurück zum Zitat Bocca N, Pileggi A, Molano RD, Marzorati S, Wu W, Bodor N et al. Soft corticosteroids for local immunosuppression: exploring the possibility for the use of loteprednol etabonate in islet transplantation. Pharmazie. 2008;63(3):226–32. Bocca N, Pileggi A, Molano RD, Marzorati S, Wu W, Bodor N et al. Soft corticosteroids for local immunosuppression: exploring the possibility for the use of loteprednol etabonate in islet transplantation. Pharmazie. 2008;63(3):226–32.
115.
Zurück zum Zitat Buchwald P, Bocca N, Marzorati S, Hochhaus G, Bodor N, Stabler C, et al. Feasibility of localized immunosuppression: 1. Exploratory studies with glucocorticoids in a biohybrid device designed for cell transplantation. Pharmazie. 2010;65(6):421–8.PubMed Buchwald P, Bocca N, Marzorati S, Hochhaus G, Bodor N, Stabler C, et al. Feasibility of localized immunosuppression: 1. Exploratory studies with glucocorticoids in a biohybrid device designed for cell transplantation. Pharmazie. 2010;65(6):421–8.PubMed
116.
Zurück zum Zitat Ahlgren U, Gotthardt M. Approaches for imaging islets: recent advances and future prospects. Adv Exp Med Biol. 2010;654:39–57.PubMedCrossRef Ahlgren U, Gotthardt M. Approaches for imaging islets: recent advances and future prospects. Adv Exp Med Biol. 2010;654:39–57.PubMedCrossRef
117.
118.
Zurück zum Zitat Medarova Z, Moore A. MRI as a tool to monitor islet transplantation. Nat Rev. 2009;5(8):444–52. Medarova Z, Moore A. MRI as a tool to monitor islet transplantation. Nat Rev. 2009;5(8):444–52.
119.
Zurück zum Zitat Eriksson O, Eich T, Sundin A, Tibell A, Tufveson G, Andersson H, et al. Positron emission tomography in clinical islet transplantation. Am J Transpl. 2009;9(12):2816–24.CrossRef Eriksson O, Eich T, Sundin A, Tibell A, Tufveson G, Andersson H, et al. Positron emission tomography in clinical islet transplantation. Am J Transpl. 2009;9(12):2816–24.CrossRef
120.
Zurück zum Zitat Eriksson O, Jahan M, Johnstrom P, Korsgren O, Sundin A, Halldin C, et al. In vivo and in vitro characterization of [18F]-FE-(+)-DTBZ as a tracer for beta-cell mass. Nucl Med Biol. 2010;37(3):357–63.PubMedCrossRef Eriksson O, Jahan M, Johnstrom P, Korsgren O, Sundin A, Halldin C, et al. In vivo and in vitro characterization of [18F]-FE-(+)-DTBZ as a tracer for beta-cell mass. Nucl Med Biol. 2010;37(3):357–63.PubMedCrossRef
121.
Zurück zum Zitat Prescher JA, Contag CH. Guided by the light: visualizing biomolecular processes in living animals with bioluminescence. Curr Opin Chem Biol. 2010;14(1):80–9.PubMedCrossRef Prescher JA, Contag CH. Guided by the light: visualizing biomolecular processes in living animals with bioluminescence. Curr Opin Chem Biol. 2010;14(1):80–9.PubMedCrossRef
122.
Zurück zum Zitat Speier S, Nyqvist D, Kohler M, Caicedo A, Leibiger IB, Berggren PO. Noninvasive high-resolution in vivo imaging of cell biology in the anterior chamber of the mouse eye. Nat Protoc. 2008;3(8):1278–86.PubMedCentralPubMedCrossRef Speier S, Nyqvist D, Kohler M, Caicedo A, Leibiger IB, Berggren PO. Noninvasive high-resolution in vivo imaging of cell biology in the anterior chamber of the mouse eye. Nat Protoc. 2008;3(8):1278–86.PubMedCentralPubMedCrossRef
123.
Zurück zum Zitat Martinic MM, von Herrath MG. Real-time imaging of the pancreas during development of diabetes. Immunol Rev. 2008;221:200–13.PubMedCrossRef Martinic MM, von Herrath MG. Real-time imaging of the pancreas during development of diabetes. Immunol Rev. 2008;221:200–13.PubMedCrossRef
124.
Zurück zum Zitat Nyman LR, Wells KS, Head WS, McCaughey M, Ford E, Brissova M, et al. Real-time, multidimensional in vivo imaging used to investigate blood flow in mouse pancreatic islets. J Clin Investig. 2008;118(11):3790–7.PubMedCentralPubMedCrossRef Nyman LR, Wells KS, Head WS, McCaughey M, Ford E, Brissova M, et al. Real-time, multidimensional in vivo imaging used to investigate blood flow in mouse pancreatic islets. J Clin Investig. 2008;118(11):3790–7.PubMedCentralPubMedCrossRef
125.
Zurück zum Zitat Molano RD, Abdulreda M, Faleo G, Molina J, Berggren PO, Ricordi C, et al. Allogeneic islet transplantation in the anterior chamber of the eye for in vivo studies of islet immunobiology. Diabetes. 2009;58:A499.CrossRef Molano RD, Abdulreda M, Faleo G, Molina J, Berggren PO, Ricordi C, et al. Allogeneic islet transplantation in the anterior chamber of the eye for in vivo studies of islet immunobiology. Diabetes. 2009;58:A499.CrossRef
127.
Zurück zum Zitat Fan Z, Spencer JA, Lu Y, Pitsillides CM, Singh G, Kim P, et al. In vivo tracking of ‘color-coded’ effector, natural and induced regulatory T cells in the allograft response. Nat Med. 2010;16(6):718–22.PubMedCentralPubMedCrossRef Fan Z, Spencer JA, Lu Y, Pitsillides CM, Singh G, Kim P, et al. In vivo tracking of ‘color-coded’ effector, natural and induced regulatory T cells in the allograft response. Nat Med. 2010;16(6):718–22.PubMedCentralPubMedCrossRef
128.
Zurück zum Zitat Abdulreda MH, Caicedo A, Berggren PO. A natural body window to study human pancreatic islet function and survival. CellR4. 2013;1(2):111–22. Abdulreda MH, Caicedo A, Berggren PO. A natural body window to study human pancreatic islet function and survival. CellR4. 2013;1(2):111–22.
Metadaten
Titel
Transdisciplinary approach to restore pancreatic islet function
verfasst von
Carmen Fotino
R. Damaris Molano
Camillo Ricordi
Antonello Pileggi
Publikationsdatum
01.12.2013
Verlag
Springer US
Erschienen in
Immunologic Research / Ausgabe 1-3/2013
Print ISSN: 0257-277X
Elektronische ISSN: 1559-0755
DOI
https://doi.org/10.1007/s12026-013-8437-4

Weitere Artikel der Ausgabe 1-3/2013

Immunologic Research 1-3/2013 Zur Ausgabe

Immunology & Microbiology in Miami

Age effects on mouse and human B cells

Eingreifen von Umstehenden rettet vor Erstickungstod!

15.05.2024 Fremdkörperaspiration Nachrichten

Wer sich an einem Essensrest verschluckt und um Luft ringt, benötigt vor allem rasche Hilfe. Dass Umstehende nur in jedem zweiten Erstickungsnotfall bereit waren, diese zu leisten, ist das ernüchternde Ergebnis einer Beobachtungsstudie aus Japan. Doch es gibt auch eine gute Nachricht.

Darf man die Behandlung eines Neonazis ablehnen?

08.05.2024 Gesellschaft Nachrichten

In einer Leseranfrage in der Zeitschrift Journal of the American Academy of Dermatology möchte ein anonymer Dermatologe bzw. eine anonyme Dermatologin wissen, ob er oder sie einen Patienten behandeln muss, der eine rassistische Tätowierung trägt.

Ein Drittel der jungen Ärztinnen und Ärzte erwägt abzuwandern

07.05.2024 Klinik aktuell Nachrichten

Extreme Arbeitsverdichtung und kaum Supervision: Dr. Andrea Martini, Sprecherin des Bündnisses Junge Ärztinnen und Ärzte (BJÄ) über den Frust des ärztlichen Nachwuchses und die Vorteile des Rucksack-Modells.

Nur selten Nachblutungen nach Abszesstonsillektomie

03.05.2024 Tonsillektomie Nachrichten

In einer Metaanalyse von 18 Studien war die Rate von Nachblutungen nach einer Abszesstonsillektomie mit weniger als 7% recht niedrig. Nur rund 2% der Behandelten mussten nachoperiert werden. Die Therapie scheint damit recht sicher zu sein.

Update HNO

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.