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International Urogynecology Journal

Erschienen in:

01.04.2013 | Original Article

Transforming growth factor beta 1 gene expression during vaginal vs cutaneous surgical wound healing in the rabbit

verfasst von: Yoram Abramov, Emmet Hirsch, Vladimir Ilievski, Roger P. Goldberg, Sylvia M. Botros, Peter K. Sand

Erschienen in: International Urogynecology Journal | Ausgabe 4/2013

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Abstract

Introduction and hypothesis

Reconstructive pelvic surgery outcome is closely related to the vaginal and pelvic wound healing processes. Transforming growth factor beta 1 (TGF-β1) is a principal mediator of wound repair in dermal tissue. We sought to assess this factor’s expression in vaginal and dermal surgical wound repair in the rabbit.

Methods

We excised bilateral 6-mm full-thickness circular segments from the abdominal skin and vagina in 36 New Zealand White (NZW) nulliparous female rabbits. Animals were sacrificed before, on the day of, and 4, 7, 10, 14, 21, 28, and 35 days after tissue wounding, and their wounds were assessed for surface area and TGF-β1 gene transcription by real-time polymerase chain reaction (PCR).

Results

In both the abdominal skin and vagina, TGF-β1 gene transcription increased immediately after tissue injury, reaching maximal levels on days 4–7, and decreased shortly thereafter, attaining minimal values on day 35. A significant correlation between TGF-β1 expression and the wound’s closure rate was found in both tissues.

Conclusions

TGF-β1 gene transcription significantly correlates with the surgical vaginal and dermal wound closure rate, implying that this factor is involved in the process of wound repair in both tissues.

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Titel: Transforming growth factor beta 1 gene expression during vaginal vs cutaneous surgical wound healing in the rabbit
verfasst von: Yoram Abramov
Emmet Hirsch
Vladimir Ilievski
Roger P. Goldberg
Sylvia M. Botros
Peter K. Sand
Publikationsdatum: 01.04.2013
Verlag: Springer-Verlag
Erschienen in: International Urogynecology Journal / Ausgabe 4/2013
Print ISSN: 0937-3462
Elektronische ISSN: 1433-3023
DOI: https://doi.org/10.1007/s00192-012-1905-x

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Transforming growth factor beta 1 gene expression during vaginal vs cutaneous surgical wound healing in the rabbit