nach oben

Erschienen in:

21.02.2019 | Original Article

Ultrastructural changes on fertile and infertile hydatid cysts induced by conventional and solid lipid nanoparticles of albendazole and albendazole sulfoxide

verfasst von: Abdollah Rafiei, Shahrzad Soltani, Zahra Ramezani, Mohammad Reza Abbaspour, Ali Jelowdar, Mehdi Sagha Kahvaz

Erschienen in: Comparative Clinical Pathology | Ausgabe 4/2019

Einloggen, um Zugang zu erhalten

Abstract

Cystic echinococcosis (CE), a serious public health problem worldwide, is caused by the larval stage of Echinococcus granulosus (E. granulosus). Despite recent progress in the CE medical treatment, this subject still remains as a priority of research. Albendazole (ABZ) is a drug of choice for the treatment of hydatidosis. Its use as an antiparasitic agent with the most efficacy is limited by the lack of water solubility. Therefore, the aim of the present study was to compare in vitro efficacy of free and solid lipid nanoparticles (SLNs) of ABZ and albendazole sulfoxide (ABZSO) against fertile and infertile hydatid cysts in terms of ultrastructural changes. ABZ-SLN and ABZSO-SLN were produced by micro emulsification and high shear homogenization technique and the physicochemical characterization of the product was evaluated. Then, the structural alterations occurring due to treatment with free and SLN formulations against fertile and infertile sheep liver hydatid cysts were assessed by optical and transmission electron microscopy (TEM) examinations. Untreated control groups revealed the typical appearance of hydatid cysts; in contrast, all treated cysts exhibited degenerative alterations with various rates. Loading ABZ and ABZSO into SLN leads to greater structural changes. In both prepared formulations, better efficacy was observed in fertile and small cysts.

Agudelo Higuita NI, Brunetti E, McCloskey C (2016) Cystic echinococcosis. J Clin Microbiol 54(3):518–523. https://doi.org/10.1128/JCM.02420-15 CrossRefPubMedPubMedCentral

Ahmadnia S, Moazeni M, Mohammadi-Samani S, Oryan A (2013) In vivo evaluation of the efficacy of albendazole sulfoxide and albendazole sulfoxide loaded solid lipid nanoparticles against hydatid cyst. Exp Parasitol 135(2):314–319. https://doi.org/10.1016/j.exppara.2013.07.017 CrossRefPubMed

Brunetti E, Kern P, Vuitton DA, Writing Panel for the W-I (2010) Expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans. Acta Trop 114(1):1–16. https://doi.org/10.1016/j.actatropica.2009.11.001 CrossRefPubMed

Budke CM, Deplazes P, Torgerson PR (2006) Global socioeconomic impact of cystic echinococcosis. Emerg Infect Dis 12(2):296–303. https://doi.org/10.3201/eid1202.050499 CrossRefPubMedPubMedCentral

Budke CM et al (2013) A systematic review of the literature on cystic echinococcosis frequency worldwide and its associated clinical manifestations. Am J Trop Med Hyg 88(6):1011–1027. https://doi.org/10.4269/ajtmh.12-0692 CrossRefPubMedPubMedCentral

Ceballos L, Elissondo C, Moreno L, Dopchiz M, Sánchez Bruni S, Denegri G, Alvarez L, Lanusse C (2008) Albendazole treatment in cystic echinococcosis: pharmacokinetics and clinical efficacy of two different aqueous formulations. Parasitol Res 103(2):355–362. https://doi.org/10.1007/s00436-008-0980-x CrossRefPubMed

Ceballos L, Elissondo C, Sanchez Bruni S, Denegri G, Lanusse C, Alvarez L (2011) Comparative performances of flubendazole and albendazole in cystic echinococcosis: ex vivo activity, plasma/cyst disposition, and efficacy in infected mice. Antimicrob Agents Chemother 55(12):5861–5867. https://doi.org/10.1128/AAC.05105-11 CrossRefPubMedPubMedCentral

Coelho JF, Ferreira PC, Alves P, Cordeiro R, Fonseca AC, Góis JR, Gil MH (2010) Drug delivery systems: advanced technologies potentially applicable in personalized treatments. EPMA J 1(1):164–209. https://doi.org/10.1007/s13167-010-0001-x CrossRefPubMedPubMedCentral

Craig PS, McManus DP, Lightowlers MW, Chabalgoity JA, Garcia HH, Gavidia CM, Gilman RH, Gonzalez AE, Lorca M, Naquira C, Nieto A, Schantz PM (2007) Prevention and control of cystic echinococcosis. Lancet Infect Dis 7(6):385–394. https://doi.org/10.1016/S1473-3099(07)70134-2 CrossRefPubMed

Dolatabadi JEN, Jamali AA, Hasanzadeh M, Omidi Y (2011) Quercetin delivery into cancer cells with single walled carbon nanotubes. Int J Biosci Biochem Bioinforma 1(1):21–25

Dvoroznakova E, Hrckova G, Boroskova Z, Velebny S, Dubinsky P (2004) Effect of treatment with free and liposomized albendazole on selected immunological parameters and cyst growth in mice infected with Echinococcus multilocularis. Parasitol Int 53(4):315–325. https://doi.org/10.1016/j.parint.2004.05.001 CrossRefPubMed

Elissondo M, Ceballos L, Dopchiz M, Andresiuk V, Alvarez L, Sánchez Bruni S, Lanusse C, Denegri G (2007) In vitro and in vivo effects of flubendazole on Echinococcus granulosus metacestodes. Parasitol Res 100(5):1003–1009. https://doi.org/10.1007/s00436-006-0381-y CrossRefPubMed

Elissondo MC, Pensel PE, Denegri GM (2013) Could thymol have effectiveness on scolices and germinal layer of hydatid cysts? Acta Trop 125(3):251–257. https://doi.org/10.1016/j.actatropica.2012.12.007 CrossRefPubMed

Ezzati Nazhad Dolatabadi J, Valizadeh H, Hamishehkar H (2015) Solid lipid nanoparticles as efficient drug and gene delivery systems: recent breakthroughs. Adv Pharm Bull 5(2):151–159. https://doi.org/10.15171/apb.2015.022 CrossRefPubMedPubMedCentral

Hemphill A et al (2010) Echinococcus metacestodes as laboratory models for the screening of drugs against cestodes and trematodes. Parasitology 137(3):569–587. https://doi.org/10.1017/S003118200999117X CrossRefPubMed

Ibrahim WM, AlOmrani AH, Yassin AE (2014) Novel sulpiride-loaded solid lipid nanoparticles with enhanced intestinal permeability. Int J Nanomedicine 9:129–144. https://doi.org/10.2147/IJN.S54413 CrossRefPubMed

Kelidari HR, Akbari J, Saeedi M (2013) Application and characterization of solid lipid nanoparticles and nanostructured lipid carriers as drug delivery systems. J Mazandaran Uni Med Sci 23(98):387–403

Khademvatan S, Yousefi E, Rafiei A, Rahdar M, Saki J (2013) Molecular characterization of livestock and human isolates of Echinococcus granulosus from south-west Iran. J Helminthol 87(2):240–244. https://doi.org/10.1017/S0022149X12000296 CrossRefPubMed

Khademvatan S, Majidiani H, Foroutan M, Hazrati Tappeh K, Aryamand S, Khalkhali HR (2018) Echinococcus granulosus genotypes in Iran: a systematic review. J Helminthol:1–8. https://doi.org/10.1017/S0022149X18000275

Khalkhali HR, Foroutan M, Khademvatan S, Majidiani H, Aryamand S, Khezri P, Aminpour A (2018) Prevalence of cystic echinococcosis in Iran: a systematic review and meta-analysis. J Helminthol 92(3):260–268. https://doi.org/10.1017/S0022149X17000463 CrossRefPubMed

Liu C, Zhang H, Yin J, Hu W (2015) In vivo and in vitro efficacies of mebendazole, mefloquine and nitazoxanide against cyst echinococcosis. Parasitol Res 114(6):2213–2222. https://doi.org/10.1007/s00436-015-4412-4 CrossRefPubMed

Mandal S, Mandal MD (2012) Human cystic echinococcosis: epidemiologic, zoonotic, clinical, diagnostic and therapeutic aspects. Asian Pac J Trop Med 5(4):253–260. https://doi.org/10.1016/S1995-7645(12)60035-2 CrossRefPubMed

McManus DP, Zhang W, Li J, Bartley PB (2003) Echinococcosis. Lancet 362(9392):1295–1304. https://doi.org/10.1016/S0140-6736(03)14573-4 CrossRefPubMed

McManus DP, Gray DJ, Zhang W, Yang Y (2012) Diagnosis, treatment, and management of echinococcosis. BMJ 344(7861):e3866. https://doi.org/10.1136/bmj.e3866 CrossRefPubMed

Moro P, Schantz PM (2009) Echinococcosis: a review. Int J Infect Dis 13(2):125–133. https://doi.org/10.1016/j.ijid.2008.03.037 CrossRefPubMed

Morris DL, Chinnery JB, Georgiou G, Stamatakis G, Golematis B (1987) Penetration of albendazole sulphoxide into hydatid cysts. Gut 28(1):75–80CrossRefPubMedPubMedCentral

Otero-Abad B, Torgerson PR (2013) A systematic review of the epidemiology of echinococcosis in domestic and wild animals. PLoS Negl Trop Dis 7(6):e2249. https://doi.org/10.1371/journal.pntd.0002249 CrossRefPubMedPubMedCentral

Perez-Serrano J, Denegri G, Casado N, Rodriguez-Caabeiro F (1997) In vivo effect of oral albendazole and albendazole sulphoxide on development of secondary echinococcosis in mice. Int J Parasitol 27(11):1341–1345CrossRefPubMed

Ravichandran R (2010) In vivo pharmacokinetic studies of albendazole nanoparticulate oral formulations for improved bioavailability. Int J Green Nanotechnol Biomed 2(1):B46–B53. https://doi.org/10.1080/1943085x.2010.488200 CrossRef

Rostami E, Kashanian S, Azandaryani AH, Faramarzi H, Dolatabadi JEN, Omidfar K (2014) Drug targeting using solid lipid nanoparticles. Chem Phys Lipids 181:56–61. https://doi.org/10.1016/j.chemphyslip.2014.03.006 CrossRefPubMed

Sadjjadi SM (2006) Present situation of echinococcosis in the Middle East and Arabic North Africa. Parasitol Int 55(Suppl):S197–S202. https://doi.org/10.1016/j.parint.2005.11.030 CrossRefPubMed

Skuhala T, Trkulja V, Runje M, Vukelic D, Desnica B (2014) Albendazolesulphoxide concentrations in plasma and hydatid cyst and prediction of parasitological and clinical outcomes in patients with liver hydatidosis caused by Echinococcus granulosus. Croat Med J 55(2):146–155CrossRefPubMedPubMedCentral

Soltani S, Rafiei A, Ramezani Z, Abbaspour MR, Jelowdar A, Kahvaz MS (2017) Evaluation of the hydatid cyst membrane permeability of albendazole and albendazole sulfoxide-loaded solid lipid nanoparticles. Jundishapur J Nat Pharm Prod 12(2):e34723. https://doi.org/10.17795/jjnpp-34723 CrossRef

de Souza MC, Marchetti JM (2012) Development of albendazole sulfoxide-loaded Eudragit microparticles: a potential strategy to improve the drug bioavailability. Adv Powder Technol 23(6):801–807CrossRef

Stojkovic M, Zwahlen M, Teggi A, Vutova K, Cretu CM, Virdone R, Nicolaidou P, Cobanoglu N, Junghanss T (2009) Treatment response of cystic echinococcosis to benzimidazoles: a systematic review. PLoS Negl Trop Dis 3(9):e524. https://doi.org/10.1371/journal.pntd.0000524 CrossRefPubMedPubMedCentral

Torgerson PR, Devleesschauwer B, Praet N, Speybroeck N, Willingham AL, Kasuga F, Rokni MB, Zhou XN, Fèvre EM, Sripa B, Gargouri N, Fürst T, Budke CM, Carabin H, Kirk MD, Angulo FJ, Havelaar A, de Silva N (2015) World Health Organization estimates of the global and regional disease burden of 11 foodborne parasitic diseases, 2010: a data synthesis. PLoS Med 12(12):e1001920. https://doi.org/10.1371/journal.pmed.1001920 CrossRefPubMedPubMedCentral

Truong Cong T et al (2008) Study on the hydatid cyst membrane: permeation of model molecules and interactions with drug-loaded nanoparticles. Int J Pharm 353(1–2):223–232. https://doi.org/10.1016/j.ijpharm.2007.11.040 CrossRefPubMed

Verma VC, Gangwar M, Yashpal M, Nath G (2013) Anticestodal activity of endophytic Pestalotiopsis sp. on protoscoleces of hydatid cyst Echinococcus granulosus. Biomed Res Int 2013:308515. https://doi.org/10.1155/2013/308515 CrossRefPubMedPubMedCentral

Wu XW, Chen XL, Zhang SJ, Zhang X, Sun H, Peng XY (2011) Pericyst may be a new pharmacological and therapeutic target for hydatid disease. Chin Med J 124(18):2857–2862PubMed

Yones DA, Taher GA, Ibraheim ZZ (2011) In vitro effects of some herbs used in Egyptian traditional medicine on viability of protoscolices of hydatid cysts. Korean J Parasitol 49(3):255–263. https://doi.org/10.3347/kjp.2011.49.3.255 CrossRefPubMedPubMedCentral

Titel: Ultrastructural changes on fertile and infertile hydatid cysts induced by conventional and solid lipid nanoparticles of albendazole and albendazole sulfoxide
verfasst von: Abdollah Rafiei
Shahrzad Soltani
Zahra Ramezani
Mohammad Reza Abbaspour
Ali Jelowdar
Mehdi Sagha Kahvaz
Publikationsdatum: 21.02.2019
Verlag: Springer London
Erschienen in: Comparative Clinical Pathology / Ausgabe 4/2019
Print ISSN: 1618-5641
Elektronische ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-019-02925-y

Springer Medizin

Ultrastructural changes on fertile and infertile hydatid cysts induced by conventional and solid lipid nanoparticles of albendazole and albendazole sulfoxide

Abstract

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 4/2019

Inorganic nanoparticles restrict viability of metastatic breast cancer cells in vitro

Comparison of transduction efficiency among various cell types by a lentivector containing CMV promoter

A comparative analysis of the molecular basis of fibrosis between tissues

Use of the turbidimetric method for determining hemoglobin (A1C) in diabetic and nondiabetic dogs

Aging of bovine spinal cord: an alteration of myelinated nerve fibers in the white matter

Sero-prevalence and associated risk factors of Mycoplasma hyopneumoniae infection in Kailali and Kanchanpur District of Far Western, Nepal

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie