Background
Methods
Microarray data
Data preprocessing
Differentially expressed genes (DEGs) screening
Construction of Weighted Gene Co-expression Network
Sun-pathway analysis of hub genes in the most significant module
Results
Data preprocessing and DEGs screening
WGCNA analysis and key modules identification
Module | Correlation |
P-value |
---|---|---|
Darkmagenta | 0.88 | 1.95E-5 |
Lavenderblush3 | 0.76 | 1.07E-3 |
Coral1 | 0.75 | 1.20E-3 |
Grey | 0.74 | 1.65E-3 |
Plum2 | 0.72 | 2.24E-3 |
Red | 0.71 | 3.05E-3 |
Mediumpurple3 | 0.7 | 3.89E-3 |
Violet | 0.68 | 5.44E-3 |
Thistle1 | 0.62 | 1.32E-2 |
Maroon | 0.58 | 2.33E-2 |
Darkseagreen4 | 0.55 | 3.44E-2 |
Ivory | 0.54 | 3.93E-2 |
Brown4 | 0.53 | 4.13E-2 |
Antiquewhite4 | 0.53 | 4.10E-2 |
Mediumorchid | −0.38 | 1.65E-1 |
Midnightblue | −0.6 | 1.75E-2 |
Brown | −0.69 | 4.45E-3 |
Black | −0.73 | 2.11E-3 |
Darkolivegreen | −0.74 | 1.66E-3 |
Floralwhite | −0.8 | 3.91E-4 |
Bisque4 | −0.87 | 2.07E-5 |
Functional and pathway enrichment analysis
Category | Term | Count |
P-value |
---|---|---|---|
KEGG_PATHWAY | hsa05410:Hypertrophic cardiomyopathy (HCM) | 3 | 2.85E-2 |
GOTERM_BP_FAT | GO:0016044~membrane organization | 5 | 1.47E-2 |
GOTERM_BP_FAT | GO:0042391~regulation of membrane potential | 3 | 4.44E-2 |
GOTERM_CC_FAT | GO:0005886~plasma membrane | 18 | 4.37E-3 |
GOTERM_CC_FAT | GO:0044459~plasma membrane part | 12 | 1.47E-2 |
GOTERM_CC_FAT | GO:0045121~membrane raft | 3 | 4.95E-2 |
Sub-pathway analysis of hub genes in darkmagenta module
Pathway Id | Pathway name |
P-value | Genes |
---|---|---|---|
path:00030_5 | Pentose phosphate pathway | 9.60E-3 | G6PD |
path:00030_4 | Pentose phosphate pathway | 2.31E-2 | G6PD |
path:00910_2 | Nitrogen metabolism | 3.12E-2 | CA7 |
path:00030_3 | Pentose phosphate pathway | 3.65E-2 | G6PD |