Introduction
Materials and methods
Chemicals
Instrumentation
Sample analysis protocol
Evaluation of matrix effects, recovery, extraction efficiency and batch stability
Correlation between 903 and FTA cards in spiked samples without biological matrix
Results
Substance | Conc. in not-dried blood (ng/mL) | Conc. in Whatman™ 903 DBS (ng/mL) | Conc. in Whatman™ FTA DBS (ng/mL) | |||
---|---|---|---|---|---|---|
Mean | SD | Mean | SD | Mean | SD | |
Cocaine (n = 11) | 56.7 | 2.6 | 63.5 | 3.3 | 62.6 | 1.8 |
302 | 25.1 | 295 | 12.5 | 295 | 10.4 | |
155 | 11.6 | 181 | 24.9 | 197 | 34.2 | |
679 | 30.7 | 686 | 163 | 670 | 76.2 | |
54.7 | 8.8 | 46.6 | 9.6 | 73.6 | 11.3 | |
52.7 | 5.3 | 50.3 | 6.4 | 57.3 | 9.2 | |
40 | 1.4 | 50.5 | 6.7 | 45.7 | 4 | |
231 | 16.4 | 231 | 4 | 244 | 13.1 | |
148 | 13.7 | 136 | 13.5 | 158 | 13.3 | |
1350 | 40 | 1340 | 65.6 | 1357 | 92.9 | |
85.3 | 3.1 | 71 | 5.8 | 70.1 | 19.6 | |
BE (n = 11) | 443 | 32.6 | 520 | 37.3 | 517 | 12.3 |
1170 | 45.1 | 1060 | 104 | 1180 | 81.9 | |
745 | 23.1 | 718 | 27.6 | 873 | 13.8 | |
2140 | 217 | 1900 | 28.6 | 2830 | 705 | |
1150 | 1230 | 1230 | 20.8 | 1280 | 145 | |
441 | 27.9 | 541 | 17.9 | 526 | 15.3 | |
87.1 | 14 | 117 | 16.8 | 105 | 5.8 | |
771 | 27.8 | 709 | 13.9 | 792 | 17.6 | |
948 | 49.2 | 1010 | 108 | 943 | 49.8 | |
4170 | 270 | 3600 | 130 | 3750 | 261 | |
3700 | 431 | 3590 | 431 | 3750 | 329 | |
EME (n = 11) | 192 | 6.4 | 178 | 25.5 | 226 | 24.9 |
608 | 7.8 | 543 | 56 | 564 | 23.8 | |
381 | 21.2 | 364 | 28.5 | 427 | 69.9 | |
2150 | 70 | 2130 | 172 | 2180 | 197 | |
476 | 22 | 403 | 61.8 | 556 | 55.3 | |
108 | 3.6 | 108 | 9 | 148 | 8.4 | |
52 | 3.8 | 59.6 | 8.7 | 64.4 | 3.7 | |
439 | 10.5 | 477 | 18.2 | 456 | 47.9 | |
335 | 7.8 | 307 | 6.6 | 335 | 8.6 | |
3120 | 138 | 3310 | 221 | 3410 | 85 | |
846 | 30 | 894 | 40.3 | 952 | 37.7 | |
CE (n = 3) | 103 | 4.3 | 102 | 12.6 | 107 | 6.4 |
104 | 11.5 | 96.6 | 10.2 | 108 | 12.7 | |
81.1 | 5.2 | 76 | 4.4 | 70.7 | 6.3 | |
Morphine (n = 4) | 34.4 | 3.3 | 34.6 | 0.7 | 33.6 | 3.4 |
206 | 7.5 | 240 | 18 | 208 | 32 | |
155 | 4.6 | 98.9 | 8.1 | 149 | 29.1 | |
237 | 11.5 | 267 | 41.6 | 299 | 23.1 | |
Codeine (n = 4) | 19.2 | 1.2 | 19.3 | 1.5 | 20.4 | 1.3 |
27.5 | 0.8 | 31.8 | 2.3 | 31.9 | 1.3 | |
21.8 | 1.5 | 22.2 | 1.1 | 27.3 | 6.6 | |
31.9 | 0.4 | 34.6 | 2.4 | 31.5 | 3.1 | |
Methadone (n = 3) | 781 | 231 | 781 | 91.1 | 527 | 147 |
1870 | 1960 | 785 | 33 | 499 | 12.5 | |
241 | 13 | 264 | 43.1 | 162 | 23.2 | |
EDDP (n = 3) | 102 | 13.2 | 56.9 | 6.2 | 50.7 | 13.6 |
158 | 169 | 60.2 | 2.5 | 49.4 | 2.5 | |
35.9 | 1.6 | 45.2 | 9.3 | 38.6 | 2.8 | |
Quetiapine (n = 2) | 276 | 30.5 | 223 | 16.5 | 268 | 44 |
6700 | 1060 | 5930 | 621 | 8730 | 1710 | |
Citalopram (n = 2) | 11.1 | 0.2 | 9.8 | 1.5 | 6.5 | 0.9 |
345 | 34.7 | 359 | 14.5 | 295 | 24 | |
Venlafaxine (n = 2) | 1810 | 233 | 2120 | 537 | 2070 | 548 |
2590 | 50.3 | 2490 | 17.7 | 2480 | 444 | |
Desvenlafaxine (n = 2) | 541 | 82.1 | 486 | 15 | 545 | 25.8 |
832 | 73.4 | 976 | 329 | 819 | 82 | |
Diazepam (n = 2) | 18.5 | 2 | 19.3 | 2.4 | 6.3 | 1.1 |
58.1 | 3.6 | 43.7 | 5.3 | 23.6 | 5.2 | |
7-Amino-clonazepam (n = 2) | 33.9 | 4.4 | 29.8 | 3.3 | 41.4 | 2.5 |
82.7 | 4.5 | 72.5 | 1.4 | 85.2 | 12.2 | |
Alprazolam (n = 1) | 37.8 | 2.9 | 34.6 | 4.9 | 18.9 | 2.5 |
Amitriptyline (n = 1) | 66.7 | 10.9 | 56.3 | 4.3 | 42.1 | 9.7 |
Bromazepam (n = 1) | 247 | 15.7 | 220 | 24.1 | 217 | 15.6 |
Desmethyldiazepam (n = 1) | 157 | 5.7 | 145 | 15.3 | 44.4 | 6.9 |
Fluoxetine (n = 1) | 1230 | 94.5 | 1310 | 95.4 | 185 | 67 |
Haloperidol (n = 1) | 7 | 0.2 | 7.9 | 0.8 | 6.5 | 0.9 |
Sertraline (n = 1) | 530 | 106 | 395 | 31.6 | 28.1 | 2 |