nach oben

Erschienen in:

17.05.2017 | Original Article

A high-fat diet can affect bone healing in growing rats

verfasst von: Jéssica Suzuki Yamanaka, Gabriela Rezende Yanagihara, Bruna Leonel Carlos, Júnia Ramos, Brígida Batista Brancaleon, Ana Paula Macedo, João Paulo Mardegan Issa, Antônio Carlos Shimano

Erschienen in: Journal of Bone and Mineral Metabolism | Ausgabe 3/2018

Einloggen, um Zugang zu erhalten

Abstract

A high-fat diet (HFD) can have a negative effect on bone quality in young and old people. Although bone healing in children is normally efficient, there is no evidence that children who have a diet rich in fat have compromised bone fracture regeneration compared with children with recommended dietary fat levels. The purpose of the present study was to evaluate the effects of an HFD on bone healing in growing female rats. Twenty-six postweaning female Wistar rats were divided into two groups (13 animals per group): a standard diet (SD) group and an HFD (with 60% of energy from fat) group. The rats received the assigned diets for 5 weeks, and in the third week they were submitted to an osteotomy procedure of the left tibia. Body mass and feed intake were recorded during the experiment. One day before euthanasia, an insulin tolerance test was performed. After euthanasia, the tibiae were removed and analyzed by densitometry, mechanical testing, histomorphometry, stereology and immunohistochemistry. An HFD caused an adaptive response to maintain energetic balance by decreasing feed intake and causing insulin insensitivity. There was no change in bone mineral density, collagen amount and immunostaining for bone formation, but maximal load and stiffness were decreased in the HFD group. In addition, bone volume had a tendency to be higher in the SD group than in the HFD group. Compared with rats receiving an SD, growing rats receiving an HFD for 5 weeks had similar bone mineral density but altered mechanical properties at the osteotomy defect site.

Azad V, Breitbart E, Al-Zube L, Azad Yeh S, O’Connor JP, Lin SS (2009) rhBMP-2 enhances the bone healing response in a diabetic rat segmental defect model. J Orthop Trauma 23:267–276CrossRefPubMed

Diniz SF, Amorim FPLG, Cavalcante-Neto FF, Bocca AL, Batista AC, Simm GEPM, Silva TA (2008) Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture. Braz J Med Biol Res 41:373–379CrossRefPubMed

Ogasawara A, Nakajima A, Nakajima F, Goto K, Yamazaki M (2008) Molecular basis for affected cartilage formation and bone union in fracture healing of the streptozotocin-induced diabetic rat. Bone 43:832–839CrossRefPubMed

Gustafson B, Hedjazifar S, Gogg S, Hammarstedt A, Smith U (2015) Insulin resistance and impaired adipogenesis. Trends Endocrinol Metab 26:193–200CrossRefPubMed

Brown ML, Yukata K, Farnsworth CW, Chen DG, Awad H, Hilton MJ, O’Keefe RJ, Xing L, Mooney RA, Zuscik MJ (2014) Delayed fracture healing and increased callus adiposity in a C57BL/6J murine model of obesity-associated type 2 diabetes mellitus. PLoS One 9:1–11

Histing T, Andonyan A, Klein M, Scheuer C, Stenger D, Holstein JH, Veith NT, Pohlemann T, Menger MD (2016) Obesity does not affect the healing of femur fractures in mice. Injury 47:1435–1444CrossRefPubMed

Woo DG, Lee BY, Lim D, Kim HS (2009) Relationship between nutrition factors and osteopenia: effects of experimental diets on immature bone quality. J Biomech 42:1102–1107CrossRefPubMed

Corwin RL (2003) Effects of dietary fats on bone health in advanced age. Prostaglandins Leukot Essent Fat Acids 68:379–386CrossRef

Greco EA, Lenzi A, Migliaccio S (2015) The obesity of bone. Ther Adv Endocrinol Metab 6:273–286CrossRefPubMedPubMedCentral

10.

Kayal RA, Alblowi J, McKenzie E, Krothapalli N, Silkman L, Gerstenfeld L, Einhorn TA, Graves DT (2009) Diabetes causes the accelerated loss of cartilage during fracture repair which is reversed by insulin treatment. Bone 44:357–363CrossRefPubMed

11.

Kawai M, Paula FJA, Rosen CJ (2012) New insights into osteoporosis: the bone-fat connection. J Intern Med 272:317–329CrossRefPubMedPubMedCentral

12.

Chaplais E, Thivel D, Greene D, Dutheil F, Duche P, Naughton G, Courteix D (2015) Bone-adiposity cross-talk: implications for pediatric obesity: a narrative review of literature. J Bone Miner Metab 33:592–602CrossRefPubMed

13.

Fornari ED, Suszter M, Roocroft J, Bastrom T, Edmonds EW, Schlechter J (2013) Childhood obesity as a risk factor for lateral condyle fractures over supracondylar humerus fractures pediatrics. Clin Orthop Relat Res 471:1193–1198CrossRefPubMed

14.

Taylor ED, Theim KR, Mirch MC, Ghorbani S, Tanofsky-kraff M, Adler-Wailes DC, Brady S, Reynolds JC, Calis KA, Pharma D, Yanovisk JA (2006) Orthopedic complications of overweight in children and adolescents. Pediatrics 117:2167–2174CrossRefPubMedPubMedCentral

15.

Goulding A, Taylor RW, Jones IE, McAuley KA, Manning PJ, Williams SM (2000) Overweight and obese children have low bone mass and area for their weight. Int J Obes Relat Metab Disord 24:627–632CrossRefPubMed

16.

World Health Organization (2014) Obesity and overweight. http://www.who.int/gho/ncd/risk_factors/overweight/en/index1.html. Accessed 29 Jun 2016

17.

Kin CFW, Shan WSY, Shun LJC, Chung LP, Jean W (2007) Experience of famine and bone health in post-menopausal women. Int J Epidemiol 36:1143–1150CrossRefPubMed

18.

Reeves PG, Nielsen FH, Fahey GC Jr (1939) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939–1951CrossRef

19.

Paiva AG, Yanagihara GR, Macedo AP, Ramos J, Issa JPM, Shimano AC (2016) Analysis of fracture healing in osteopenic bone caused by disuse: experimental study. Braz J Med Biol Res 49:3–9

20.

Sartori AR, Moreira JA, Santos AMM, Cintra DEC, Sartori LR, Baraúna MA, Canto RST (2008) Bone repair process in normal and osteopenic female rats’ tibiae: a comparative study. Acta Ortop Bras 16:37–40CrossRef

21.

Parfitt AM, Drezner MK, Glorieux FH, Kanis JA, Malluche H, Meunier PJ, Ott SM, Recker RR (1987) Bone histomorphometry: standardization of nomenclature, symbols, and units. Bone Miner Res 2:595–610CrossRef

22.

Gautam J, Choudhary D, Khedgikar V, Kushwaha P, Singh RS, Singh D, Tiwari S, Trivedi R (2014) Micro-architectural changes in cancellous bone differ in female and male C57BL/6 mice with high-fat diet-induced low bone mineral density. Br J Nutr 111:1811–1821CrossRefPubMed

23.

Silva HGV, Mendonca LMC, Conceicao FL, Zahar SEV, Farias MLF (2007) Influence of obesity on bone density in postmenopausal women. Arq Bras Endocrinol Metab 51:943–949CrossRef

24.

Frost HM (1997) Obesity, and bone strength and “mass”: a tutorial based on insights from a new paradigm. Bone 21:211–214CrossRefPubMed

25.

Ionova-Martin SS, Do SH, Barth HD, Szadkowska M, Porter AE, Iii JWA, Ager JW Jr, Alliston T, Vaisse C, Ritchie RO (2010) Reduced size-independent mechanical properties of cortical bone in high-fat diet-induced obesity. Bone 46:217–225CrossRefPubMed

26.

Beresford JN, Bennett JH, Devlin C, Leboy PS, Owen ME (1992) Evidence for an inverse relationship between the differentiation of adipocytic and osteogenic cells in rat marrow stromal cell cultures. J Cell Sci 102:341–351PubMed

27.

Nuttall ME, Patton AJ, Olivera DL, Nadeau DP, Gowen M (1998) Human trabecular bone cells are able to express both osteoblastic and adipocytic phenotype: implications for osteopenic disorders. J Bone Miner Res 13:371–382CrossRefPubMed

28.

Rosen ED, MacDougald O (2006) Adipocyte differentiation from the inside out. Nat Rev Mol Cell Biol 7:885–896CrossRefPubMed

29.

Cao JJ, Gregoire BR (2016) A high-fat diet increases body weight and circulating estradiol concentrations but does not improve bone structural properties in ovariectomized mice. Nutr Res 36:320–327CrossRefPubMed

30.

Hammer A (2015) The paradox of Wolff’s theories. Ir J Med Sci 184:13–22CrossRefPubMed

31.

Vendrell J, Broch M, Vilarrasa N, Molina A, Gómez JM, Gutiérrez C, Simón I, Soler J, Richart C (2004) Resistin, adiponectin, ghrelin, leptin, and proinflammatory cytokines: relationships in obesity. Obes Res 12:962–971CrossRefPubMed

32.

Rendina-Ruedy E, Graef JL, Davis MR, Hembree KD, Gimble JM, Clarke SL, Lucas EA, Smith BJ (2016) Strain differences in the attenuation of bone accrual in a young growing mouse model of insulin resistance. J Bone Miner Metab 34:380–394CrossRefPubMed

33.

Jones E, Yang X (2011) Mesenchymal stem cells and bone regeneration: current status. Injury 42:562–568CrossRefPubMed

34.

Peel N (2009) Bone remodelling and disorders of bone metabolism. Surgery 27:70–74

35.

Sharir A, Barak MM, Shahar R (2008) Whole bone mechanics and mechanical testing. Vet J 177:8–17CrossRefPubMed

36.

Wang Y, Dellatore P, Douard V, Qin L, Watford M, Ferraris RP, Lin T, Shapses SA (2016) High fat diet enriched with saturated, but not monounsaturated fatty acids adversely affects femur, and both diets increase calcium absorption in older female mice. Nutr Res 36:742–750CrossRefPubMedPubMedCentral

37.

Macedo AP, Shimano RC, Ferrari DT, Issa JPM, Jordão AA, Shimano AC (2016) Influence of treadmill training on bone structure under osteometabolic alteration in rats subjected to high-fat diet. Scand J Med Sci Sport. doi:10.1111/sms.12650

38.

Shen CL, Cao JJ, Dagda RY, Chanjaplammootil S, Lu C, Chyu MC, Gao W, Wang JS, Yeh JK (2012) Green tea polyphenols benefits body composition and improves bone quality in long-term high-fat diet-induced obese rats. Nutr Res 32:448–457CrossRefPubMed

39.

Premaor MO, Lesley P, Carol T, Parker RA, Compston J (2010) Obesity and fractures in postmenopausal women. Bone Miner Res 25:292–297CrossRef

40.

Tang QQ, Lane MD (2012) Adipogenesis: from stem cell to adipocyte. Annu Rev Biochem 81:715–736CrossRefPubMed

41.

Ralstson S (2005) Structure and metabolism of bone. Calcium Bone 33:58–60

42.

Issa JPM, Defino HLA, Sebald W, Coutinho-Netto J, Iyomasa MM, Shimano AC, Bentley MVLB, Pitol DL (2012) Biological evaluation of the bone healing process after application of two potentially osteogenic proteins: an animal experimental model. Gerodontology 29:258–264CrossRefPubMed

43.

Fiorino P, Américo ALV, Muller CR, Evangelista FS, Santos F, Leite APO, Farah V (2016) Exposure to high-fat diet since post-weaning induces cardiometabolic damage in adult rats. Life Sci 160:12–17CrossRefPubMed

44.

Jackman MR, MacLean PS, Bessesen DH (2010) Energy expenditure in obesity-prone and obesity-resistant rats before and after the introduction of a high-fat diet. Am J Physiol Regul Integr Comp Physiol 299:1097–1105CrossRef

45.

Higa TS, Spinola AV, Fonseca-Alaniz MH, Anna Evangelista FS (2014) Comparison between cafeteria and high-fat diets in the induction of metabolic dysfunction in mice. Int J Physiol Pathophysiol Pharmacol 6:47–54PubMedPubMedCentral

46.

Zhu K, Hunter M, James A, Lim EM, Cooke BR, Walsh JP (2016) Discordance between fat mass index and body mass index is associated with reduced bone mineral density in women but not in men: the Busselton Healthy Ageing Study. Osteoporos Int 28:259–268CrossRefPubMed

47.

Rutkowski JM, Stern JH, Scherer PE (2015) The cell biology of fat expansion. J Cell Biol 208:501–512CrossRefPubMedPubMedCentral

48.

Cao JJ, Gregoire BR, Gao H (2009) High-fat diet decreases cancellous bone mass but has no effect on cortical bone mass in the tibia in mice. Bone 44:1097–1104CrossRefPubMed

49.

Malvi P, Piprode V, Chaube B, Pote ST, Mittal M, Chattopadhyay N, Wani MR, Bhat MK (2014) High fat diet promotes achievement of peak bone mass in young rats. Biochem Biophys Res Commun 455:133–138CrossRefPubMed

Titel: A high-fat diet can affect bone healing in growing rats
verfasst von: Jéssica Suzuki Yamanaka
Gabriela Rezende Yanagihara
Bruna Leonel Carlos
Júnia Ramos
Brígida Batista Brancaleon
Ana Paula Macedo
João Paulo Mardegan Issa
Antônio Carlos Shimano
Publikationsdatum: 17.05.2017
Verlag: Springer Japan
Erschienen in: Journal of Bone and Mineral Metabolism / Ausgabe 3/2018
Print ISSN: 0914-8779
Elektronische ISSN: 1435-5604
DOI: https://doi.org/10.1007/s00774-017-0837-4

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

Notfall-TEP der Hüfte ist auch bei 90-Jährigen machbar

26.04.2024 Hüft-TEP Nachrichten

Ob bei einer Notfalloperation nach Schenkelhalsfraktur eine Hemiarthroplastik oder eine totale Endoprothese (TEP) eingebaut wird, sollte nicht allein vom Alter der Patientinnen und Patienten abhängen. Auch über 90-Jährige können von der TEP profitieren.

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

25.04.2024 Hypotonie Nachrichten

Wenn unter einer medikamentösen Hochdrucktherapie der diastolische Blutdruck in den Keller geht, steigt das Risiko für schwere kardiovaskuläre Ereignisse: Darauf deutet eine Sekundäranalyse der SPRINT-Studie hin.

Bei schweren Reaktionen auf Insektenstiche empfiehlt sich eine spezifische Immuntherapie

25.04.2024 Allergien und Intoleranzreaktionen Nachrichten

Insektenstiche sind bei Erwachsenen die häufigsten Auslöser einer Anaphylaxie. Einen wirksamen Schutz vor schweren anaphylaktischen Reaktionen bietet die allergenspezifische Immuntherapie. Jedoch kommt sie noch viel zu selten zum Einsatz.

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

25.04.2024 Hypertonie Nachrichten

Beginnen ältere Männer im Pflegeheim eine Antihypertensiva-Therapie, dann ist die Frakturrate in den folgenden 30 Tagen mehr als verdoppelt. Besonders häufig stürzen Demenzkranke und Männer, die erstmals Blutdrucksenker nehmen. Dafür spricht eine Analyse unter US-Veteranen.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 3/2018

Effect of radiation dose reduction on texture measures of trabecular bone microstructure: an in vitro study

Decreased sensory nerve excitation and bone pain associated with mouse Lewis lung cancer in TRPV1-deficient mice

Effects of C-myc gene silencing on interleukin-1β-induced rat chondrocyte cell proliferation, apoptosis and cytokine expression

Serum CTX levels and histomorphometric analysis in Src versus RANKL knockout mice

Physicochemical characterization of mineral deposits in human ligamenta flava