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01.12.2014 | Original Article

A hydrolyzed whey peptide-based diet ameliorates hepatic ischemia–reperfusion injury in the rat nonalcoholic fatty liver

verfasst von: Akira Nii, Tohru Utsunomiya, Mitsuo Shimada, Toru Ikegami, Hiroki Ishibashi, Satoru Imura, Yuji Morine, Tetsuya Ikemoto, Hajime Sasaki, Akihiro Kawashima

Erschienen in: Surgery Today | Ausgabe 12/2014

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Abstract

Purposes

The number of patients with nonalcoholic fatty liver disease (NAFLD) is increasing. Hepatic steatosis is a major risk factor for hepatic failure after ischemia–reperfusion (I/R) injury. Hydrolyzed whey peptide (HWP) is a functional liquid-type nutritional diet containing whey peptide, which has previously been shown to exert anti-inflammatory effects. In the present study, we examined the effects of HWP on the hepatic I/R injury in a rat NAFLD model.

Methods

Rats fed a methionine/choline-deficient diet for 4 weeks were divided into two groups after 30 min of whole liver ischemia. In Group-M, HWP was given immediately after reperfusion and every 6 h thereafter. In Group-C, the vehicle was given in the same manner. The liver function tests and microscopic findings of the liver after reperfusion were compared between the two groups.

Results

The serum transaminase levels in Group-M were significantly lower than those in Group-C after reperfusion. The gene expression levels of IL-6 and inducible nitric oxide synthase (iNOS) were significantly lower in Group-M compared to Group-C. The TNF-α and uncoupling protein-2 (UCP-2) expression levels were also markedly lower in Group-M. The hepatic necrotic areas in Group-M were significantly smaller than those in Group-C.

Conclusion

The administration of a HWP diet ameliorated the hepatic I/R injury in rats with NAFLD.

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Titel: A hydrolyzed whey peptide-based diet ameliorates hepatic ischemia–reperfusion injury in the rat nonalcoholic fatty liver
verfasst von: Akira Nii
Tohru Utsunomiya
Mitsuo Shimada
Toru Ikegami
Hiroki Ishibashi
Satoru Imura
Yuji Morine
Tetsuya Ikemoto
Hajime Sasaki
Akihiro Kawashima
Publikationsdatum: 01.12.2014
Verlag: Springer Japan
Erschienen in: Surgery Today / Ausgabe 12/2014
Print ISSN: 0941-1291
Elektronische ISSN: 1436-2813
DOI: https://doi.org/10.1007/s00595-014-0853-0

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Abstract

Purposes

Methods

Results

Conclusion

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