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Erschienen in: Endocrine 1/2009

01.02.2009 | Original Paper

Ablation of NF-κB expression by small interference RNA prevents the dysfunction of human umbilical vein endothelial cells induced by high glucose

verfasst von: Gang Chen, Xiaoyan Shen, Jin Yao, Feng Chen, Xu Lin, Yufang Qiao, Tingting You, Fenghui Lin, Xiaowen Fang, Xin Zou, Lixiang Lin

Erschienen in: Endocrine | Ausgabe 1/2009

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Abstract

Diabetes is a major independent risk factor for cardiovascular disease and stroke. High glucose (HG) reduces endothelial cell (EC) proliferation with a concomitant increase in apoptosis. HG also induces the translocation of nuclear factor (NF)-κB in human umbilical vein endothelial cells (HUVECs). However, data regarding the relationship between NF-κB signaling and HG-induced endothelial dysfunction are limited. In the present study, we constructed an NF-κB-targeting RNA interference (RNAi) adenovirus vector and cultured HUVECs in 5.5, 20.5, or 30.5 mM d-glucose or in daily alternating 5.5 or 30.5 mM d-glucose. We assessed the effects of the NF-κB pathway on proliferation under HG conditions by measuring bromodeoxyuridine incorporation and conducting methyl thiazolyltetrazolium assays. We also tested apoptosis by performing flow cytometry and terminal deoxynucleotidyl transferase nick-end labeling assay. The RNAi adenovirus effectively downregulated expression of the p65 protein in HUVECs for more than 6 days. Blockage of the NF-κB pathway with the RNAi adenovirus substantially protected HUVECs from decreased proliferation and reduced cellular apoptosis in HG conditions. These findings may explain how hyperglycemia promotes dysfunction of ECs and could elucidate a potential new target for therapeutic interventions.
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Metadaten
Titel
Ablation of NF-κB expression by small interference RNA prevents the dysfunction of human umbilical vein endothelial cells induced by high glucose
verfasst von
Gang Chen
Xiaoyan Shen
Jin Yao
Feng Chen
Xu Lin
Yufang Qiao
Tingting You
Fenghui Lin
Xiaowen Fang
Xin Zou
Lixiang Lin
Publikationsdatum
01.02.2009
Verlag
Springer US
Erschienen in
Endocrine / Ausgabe 1/2009
Print ISSN: 1355-008X
Elektronische ISSN: 1559-0100
DOI
https://doi.org/10.1007/s12020-008-9120-8

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Intervallfasten zur Regeneration des Herzmuskels?

14.05.2024 Herzinfarkt Nachrichten

Die Nahrungsaufnahme auf wenige Stunden am Tag zu beschränken, hat möglicherweise einen günstigen Einfluss auf die Prognose nach akutem ST-Hebungsinfarkt. Darauf deutet eine Studie an der Uniklinik in Halle an der Saale hin.

Klimaschutz beginnt bei der Wahl des Inhalators

14.05.2024 Klimawandel Podcast

Auch kleine Entscheidungen im Alltag einer Praxis können einen großen Beitrag zum Klimaschutz leisten. Die neue Leitlinie zur "klimabewussten Verordnung von Inhalativa" geht mit gutem Beispiel voran, denn der Wechsel vom klimaschädlichen Dosieraerosol zum Pulverinhalator spart viele Tonnen CO2. Leitlinienautor PD Dr. Guido Schmiemann erklärt, warum nicht nur die Umwelt, sondern auch Patientinnen und Patienten davon profitieren.

Zeitschrift für Allgemeinmedizin, DEGAM

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