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Pediatric Surgery International

Erschienen in:

01.02.2013 | Original Article

Abnormal control of lung branching in experimental esophageal atresia

verfasst von: Ana Catarina Fragoso, Rosa Aras-Lopez, Leopoldo Martinez, José Estevão-Costa, Juan A. Tovar

Erschienen in: Pediatric Surgery International | Ausgabe 2/2013

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Abstract

Purpose

Esophageal atresia and tracheo-esophageal fistula (EA-TEF) result from abnormal division of the foregut into esophagus and trachea thus, it may influence airway branching and lung development. The present study examined lung morphogenesis in fetuses with EA-TEF focusing in the expression of FGF10 and its receptor FGFR2 IIIb.

Methods

Pregnant rats received either 1.75 mg/kg i.p. adriamycin or vehicle on E7, E8 and E9. Embryos were recovered at E15, E18 and E21 and lungs processed for immunohistochemistry and RT-PCR. Three groups were studied: control, adriamycin-exposed with EA-TEF, and adriamycin-exposed without EA-TEF. Comparisons were performed with Mann–Whitney or t tests (significance level, 5 %).

Results

Lung weight at E15 and E18 were significantly lower in adriaEA fetuses in which the relative mRNA levels of FGF10 were significantly higher. These differences disappeared near term. The receptor FGFR2 IIIb messenger was only significantly increased in adria noEA fetuses at E15. Immunohistochemical study was consistent with these findings.

Conclusions

Abnormal expression of FGF10 during earlier stages of development, when the lungs are smaller than controls, suggests a compensatory response aimed at “catching up” delayed tracheobronchial branching. Whether similar changes take place in the human condition and influence respiratory physiology remain to be determined.

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Titel: Abnormal control of lung branching in experimental esophageal atresia
verfasst von: Ana Catarina Fragoso
Rosa Aras-Lopez
Leopoldo Martinez
José Estevão-Costa
Juan A. Tovar
Publikationsdatum: 01.02.2013
Verlag: Springer-Verlag
Erschienen in: Pediatric Surgery International / Ausgabe 2/2013
Print ISSN: 0179-0358
Elektronische ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-012-3195-2

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