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05.09.2018 | Original Article

Activation of angiotensin type 2 (AT2) receptors prevents myocardial hypertrophy in Zucker diabetic fatty rats

verfasst von: Giovanna Castoldi, Cira R. T. di Gioia, Francesca Roma, Raffaella Carletti, Giuseppina Manzoni, Andrea Stella, Gianpaolo Zerbini, Gianluca Perseghin

Erschienen in: Acta Diabetologica | Ausgabe 1/2019

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Abstract

Aims

Compound 21 (C21), selective AT2 receptor agonist, has cardioprotective effects in experimental models of hypertension and myocardial infarction. The aims of the study was to evaluate the effect of C21, losartan, or both in Zucker diabetic fatty (ZDF) rats (type 2 diabetes) on (1) the prevention of myocardial hypertrophy; (2) myocardial expression of phosphatase and tensin homolog (PTEN), a target gene of miR-30a-3p, involved in myocardial remodelling.

Methods

Experiments were performed in ZDF (n = 33) and in control Lean (8) rats. From the 6th to the 20th week of age, we administered C21 (0.3 mg/kg/day) to 8 ZDF rats. 8 ZDF rats were treated with losartan (10 mg/kg/day), 8 rats underwent combination treatment, C21+ losartan, and 9 ZDF rats were left untreated. Blood glucose and blood pressure were measured every 4 weeks. At the end of the study the hearts were removed, the apex was cut for the quantification of PTEN mRNA and miR-30a-3p expression (realtime-PCR). Myocardial hypertrophy was evaluated by histomorphometric analysis, and nitrotyrosine expression (as marker of oxidative stress) by immunohistochemistry.

Results

ZDF rats had higher blood glucose (p < 0.0001) with respect to control Lean rats, while blood pressure did not change. Both parameters were not modified by C21 treatment, while losartan and losartan + C21 reduced blood pressure in ZDF rats (p < 0.05). miR-30a-3p expression was increased in ZDF rats (p < 0.01) and PTEN mRNA expression was decreased (p < 0.05). ZDF rats developed myocardial hypertrophy (p < 0.01) and increased oxidative stress (p < 0.01), both were prevented by C21 or losartan, or combination treatment. C21 or losartan normalized the expression of miR-30a-3p and PTEN.

Conclusions

Activation of AT2 receptors or AT1 receptor blockade prevents the development of myocardial hypertrophy in ZDF rats. This occurs through the modulation of the miR-30a-3p/PTEN interaction.

Hu FB (2011) Globalization of diabetes. The role of diet, lifestyle, and genes. Diabetes Care 34:1249–1257. https://doi.org/10.2337/dc11-0442 CrossRefPubMedPubMedCentral

Zimmet PZ, Magliano DJ, Herman WH, Shaw JE (2014) Diabetes: a 21st century challenge. Lancet Diabetes Endocrinol 2:56–64. https://doi.org/10.1016/S2213-8587(13)70112-8 CrossRefPubMed

UKPDS Group (1998) Tight blood pressure control and risk of macrovascular and microvascular complications in type 2 diabetes: UKPDS 38. BMJ 317:703–713CrossRef

Lorber D (2014) Importance of cardiovascular disease risk management in patients with type 2 diabetes mellitus. Diabetes Metab Syndr Obes 7:169–183. https://doi.org/10.2147/DMSO.S61438 CrossRefPubMedPubMedCentral

Jia G, Whaley-Connell A, Sowers JR (2018) Diabetic cardiomyopathy: a hyperglycaemia and insulin-resistance-induced heart disease. Diabetologia 61:21–28. https://doi.org/10.1007/s00125-017-4390-4 CrossRefPubMed

Dhalla NS, Liu X, Panagia V, Takeda N (1998) Subcellular remodeling and heart dysfunction in chronic diabetes. Cardiovasc Res 40:239–247CrossRefPubMed

Mancia G, Fagard R, Narkiewicz K et al (2013) 2013 ESH/ESC guidelines for the management of arterial hypertension: the task force for the management of arterial hypertension of the European Society of Hypertension (ESH) and of the European Society of Cardiology (ESC). J Hypertens 31:1281–1357. https://doi.org/10.1097/01.hjh.0000431740.32696.cc CrossRefPubMed

Borghi C, SIIA Task Force, Rossi F, SIF Task Force (2015) Role of the renin-angiotensin-aldosterone system and its pharmacological inhibitors in cardiovascular diseases: complex and critical issues. High Blood Press Cardiovasc Prev 22:429–444. https://doi.org/10.1007/s40292-015-0120-5 CrossRefPubMed

McCarthy CA, Widdop RE, Denton KM, Jones ES (2013) Update on the angiotensin AT2 receptor. Curr Hypertens Rep 15:25–30. https://doi.org/10.1007/s11906-012-0321-4 CrossRefPubMed

10.

Ali Q, Wu Y, Hussain T (2013) Chronic AT2 receptor activation increases renal ACE2 activity, attenuates AT1 receptor function and blood pressure in obese Zucker rats. Kidney Int 84:931–939. https://doi.org/10.1038/ki.2013.193 CrossRefPubMedPubMedCentral

11.

Rompe F, Artuc M, Hallberg A et al (2010) Direct angiotensin II type 2 receptor stimulation acts anti-inflammatory through epoxyeicosatrienoic acid and inhibition of nuclear factor kappaB. Hypertension 55:924–931. https://doi.org/10.1161/HYPERTENSIONAHA.109.147843 CrossRefPubMed

12.

Kaschina E, Grzesiak A, Li J et al (2008) Angiotensin II type 2 receptor stimulation: a novel option of therapeutic interference with the renin-angiotensin system in myocardial infarction? Circulation 118:2523–2532. https://doi.org/10.1161/CIRCULATIONAHA.108.784868 CrossRefPubMed

13.

Lauer D, Slavic S, Sommerfeld M et al (2014) Angiotensin type 2 receptor stimulation ameliorates left ventricular fibrosis and dysfunction via regulation of tissue inhibitor of matrix metalloproteinase 1/matrix metalloproteinase 9 axis and transforming growth factor β1 in the rat heart. Hypertension 63:e60–e67. https://doi.org/10.1161/HYPERTENSIONAHA.113.02522 CrossRefPubMed

14.

Unger T, Dahlöf B (2010) Compound 21, the first orally active, selective agonist of the angiotensin type 2 receptor (AT2): implications for AT2 receptor research and therapeutic potential. J Renin Angiotensin Aldosterone Syst 11:75–77. https://doi.org/10.1177/1470320309347792 CrossRefPubMed

15.

Kaschina E, Namsolleck P, Unger T (2017) AT2 receptors in cardiovascular and renal diseases. Pharmacol Res 125:39–47. https://doi.org/10.1016/j.phrs.2017.07.008 CrossRefPubMed

16.

Castoldi G, di Gioia CR, Carletti R et al (2016) Angiotensin type-2 (AT-2)-receptor activation reduces renal fibrosis in cyclosporine nephropathy: evidence for blood-pressure independent effect. Biosci Rep 36:e00403. https://doi.org/10.1042/BSR20160278 PubMedPubMedCentralCrossRef

17.

Paulis L, Becker ST, Lucht K et al (2012) Direct angiotensin II type 2 receptor stimulation in Nω-nitro-L-arginine-methyl ester-induced hypertension. The effect on pulse wave velocity and aortic remodeling. Hypertension 59:485–492. https://doi.org/10.1161/HYPERTENSIONAHA.111.185496 CrossRefPubMed

18.

Rehman A, Leibowitz A, Yamamoto N, Rautureau Y, Paradis P, Schiffrin EL (2012) Angiotensin type 2 receptor agonist compound 21 reduces vascular injury and myocardial fibrosis in stroke-prone spontaneously hypertensive rats. Hypertension 59:291–299. https://doi.org/10.1161/HYPERTENSIONAHA.111.180158 CrossRefPubMed

19.

Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281–297CrossRefPubMed

20.

Huntzinger E, Izaurralde E (2011) Gene silencing by microRNAs: contributions of translational repression and mRNA decay. Nat Rev Genet 12:99–110. https://doi.org/10.1038/nrg2936 CrossRefPubMed

21.

Guo H, Ingolia NT, Weissman JS, Bartel DP (2010) Mammalian microRNAs predominantly act to decrease target mRNA levels. Nature 466:835–840. https://doi.org/10.1038/nature09267 CrossRefPubMedPubMedCentral

22.

Duisters RF, Tijsen AJ, Schroen B et al (2009) miR-133 and miR-30 regulate connective tissue growth factor: implications for a role of microRNAs in myocardial matrix remodeling. Circ Res 104:170–178. https://doi.org/10.1161/CIRCRESAHA.108.182535 CrossRefPubMed

23.

Castoldi G, Di Gioia CR, Bombardi C et al (2012) MiR-133a regulates collagen 1A1: potential role of miR-133a in myocardial fibrosis in angiotensin II-dependent hypertension. J Cell Physiol 227:850–856. https://doi.org/10.1002/jcp.22939 CrossRefPubMed

24.

Guan X, Wang L, Liu Z et al (2016) miR-106a promotes cardiac hypertrophy by targeting mitofusin 2. J Mol Cell Cardiol 99:207–217. https://doi.org/10.1016/j.yjmcc.2016.08.016 CrossRefPubMed

25.

Zheng D, Ma J, Yu Y et al (2015) Silencing of miR-195 reduces diabetic cardiomyopathy in C57BL/6 mice. Diabetologia 58:1949–1958. https://doi.org/10.1007/s00125-015-3622-8 CrossRefPubMedPubMedCentral

26.

Yang SJ, Yang SY, Wang DD et al (2017) The miR-30 family: versatile players in breast cancer. Tumor Biol 39:1–13. https://doi.org/10.1177/1010428317692204 CrossRef

27.

Pan W, Zhong Y, Cheng C et al (2013) MiR-30-regulated autophagy mediates angiotensin II-induced myocardial hypertrophy. PLoS One 8:e53950. https://doi.org/10.1371/journal.pone.0053950 CrossRefPubMedPubMedCentral

28.

Yin X, Peng C, Ning W et al (2013) miR-30a downregulation aggravates pressure overload-induced cardiomyocyte hypertrophy. Mol Cell Biochem 379:1–6. https://doi.org/10.1007/s11010-012-1552-z CrossRefPubMed

29.

Peterson RG, Shaw WN, Neel MA, Little LA, Eichberg J (1990) Zucker diabetic fatty rat as a model for non-insulin dependent diabetes mellitus. ILAR J 32:16–19. https://doi.org/10.1093/ilar.32.3.16 CrossRef

30.

King AJ (2012) The use of animal models in diabetes research. Br J Pharmacol 166:877–894. https://doi.org/10.1111/j.1476-5381.2012.01911.x CrossRefPubMedPubMedCentral

31.

Castoldi G, di Gioia CR, Bombardi C et al (2014) Prevention of diabetic nephropathy by compound 21, selective agonist of angiotensin type 2 receptors, in Zucker diabetic fatty rats. Am J Physiol Renal Physiol 307:F1123–F1131. https://doi.org/10.1152/ajprenal.00247.2014 CrossRefPubMed

32.

Castoldi G, di Gioia CR, Giollo F et al (2016) Different regulation of miR-29a-3p in glomeruli and tubules in an experimental model of angiotensin II-dependent hypertension: potential role in renal fibrosis. Clin Exp Pharmacol Physiol 43:335–342. https://doi.org/10.1111/1440-1681.12532 CrossRefPubMed

33.

Karginov FV, Hannon GJ (2013) Remodeling of Ago2-mRNA interactions upon cellular stress reflects miRNA complementarity and correlates with altered translation rates. Genes Develop 27:1624–1632. https://doi.org/10.1101/gad.215939.113 CrossRefPubMedPubMedCentral

34.

Tate M, Grieve DJ, Ritchie RH (2017) Are targeted therapies for diabetic cardiomyopathy on the horizon? Clin Sci 131:897–915. https://doi.org/10.1042/CS20160491 CrossRef

35.

Paulis L, Foulquier S, Namsolleck P, Recarti C, Steckelings UM, Unger T (2016) Combined angiotensin receptor modulation in the management of cardio-metabolic disorders. Drugs 76:1–12. https://doi.org/10.1007/s40265-015-0509-4 CrossRefPubMed

36.

Shum M, Pinard S, Guimond MO et al (2013) Angiotensin II type 2 receptor promotes adipocyte differentiation and restores adipocyte size in high-fat/high-fructose diet-induced insulin resistance in rats. Am J Physiol Endocrinol Metab 304(2):E197–E210. https://doi.org/10.1152/ajpendo.00149.2012 CrossRefPubMed

37.

Ohshima K, Mogi M, Jing F et al (2012) Direct angiotensin II type 2 receptor stimulation ameliorates insulin resistance in type 2 diabetes mice with PPARγ activation. PLoS One 7(11):e48387. https://doi.org/10.1371/journal.pone.0048387 CrossRefPubMedPubMedCentral

38.

Shao C, Yu L, Gao L (2014) Activation of angiotensin type 2 receptors partially ameliorates streptozotocin-induced diabetes in male rats by islet protection. Endocrinology 155(3):793–804. https://doi.org/10.1210/en.2013-1601 CrossRefPubMed

39.

Guo R, Nair S (2017) Role of microRNA in diabetic cardiomyopathy: from mechanism to intervention. BBA Mol Basis Dis 1863:2070–2077. https://doi.org/10.1016/j.bbadis.2017.03.013 CrossRef

40.

Kato M, Natarajan R (2015) MicroRNAs in diabetic nephropathy: functions, biomarkers, and therapeutic targets. Ann N Y Acad Sci 1353:72–88. https://doi.org/10.1111/nyas.12758 CrossRefPubMedPubMedCentral

41.

Wang W, Lin H, Zhou L et al (2014) MicroRNA-30a-3p inhibits tumor proliferation, invasiveness and metastasis and is downregulated in hepatocellular carcinoma. EJSO 40:1586–1594. https://doi.org/10.1016/j.ejso.2013.11.008 CrossRefPubMed

42.

Li W, Dai H, Ou Q, Zuo GQ, Liu CA (2016) Overexpression of microRNA-30a-5p inhibits liver cancer cell proliferation and induces apoptosis by targeting MTDH/PTEN/AKT pathway. Tumor Biol 37:5885–5895. https://doi.org/10.1007/s13277-015-4456-1 CrossRef

43.

Oudit GY, Penninger JM (2009) Cardiac regulation by phosphoinositide 3-kinases and PTEN. Cardiovasc Res 82:250–260. https://doi.org/10.1093/cvr/cvp014 CrossRefPubMed

44.

Gao S, Park BM, Cha SA, Park WH, Park BH, Kim SH (2013) Angiotensin AT2 receptor agonist stimulates high stretch induced- ANP secretion via PI3K/NO/sGC/PKG/pathway. Peptides 47:36–44. https://doi.org/10.1016/j.peptides.2013.06.008 CrossRefPubMed

45.

Savoia C, Ebrahimian T, He Y, Gratton JP, Schiffrin EL, Touyz RM (2006) Angiotensin II/AT2 receptor-induced vasodilation in stroke-prone spontaneously hypertensive rats involves nitric oxide and cGMP-dependent protein kinase. J Hypertens 24(12):2417–2422. https://doi.org/10.1097/01.hjh.0000251902.85675.7e CrossRefPubMed

46.

Sánchez A, Contreras C, Climent B et al (2018) Impaired Ca₂₊ handling in resistance arteries from genetically obese Zucker rats: Role of the PI3K, ERK1/2 and PKC signaling pathways. Biochem Pharmacol 152:114–128. https://doi.org/10.1016/j.bcp.2018.03.020 CrossRefPubMed

Titel: Activation of angiotensin type 2 (AT2) receptors prevents myocardial hypertrophy in Zucker diabetic fatty rats
verfasst von: Giovanna Castoldi
Cira R. T. di Gioia
Francesca Roma
Raffaella Carletti
Giuseppina Manzoni
Andrea Stella
Gianpaolo Zerbini
Gianluca Perseghin
Publikationsdatum: 05.09.2018
Verlag: Springer Milan
Erschienen in: Acta Diabetologica / Ausgabe 1/2019
Print ISSN: 0940-5429
Elektronische ISSN: 1432-5233
DOI: https://doi.org/10.1007/s00592-018-1220-1

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