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European Journal of Applied Physiology

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01.12.2013 | Original Article

Acute low-load resistance exercise with and without blood flow restriction increased protein signalling and number of satellite cells in human skeletal muscle

verfasst von: Mathias Wernbom, William Apro, Gøran Paulsen, Tormod S. Nilsen, Eva Blomstrand, Truls Raastad

Erschienen in: European Journal of Applied Physiology | Ausgabe 12/2013

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Abstract

Purpose

To investigate hypertrophic signalling after a single bout of low-load resistance exercise with and without blood flow restriction (BFR).

Methods

Seven subjects performed unilateral knee extensions at 30 % of their one repetition maximum. The subjects performed five sets to failure with BFR on one leg, and then repeated the same amount of work with the other leg without BFR. Biopsies were obtained from m. vastus lateralis before and 1, 24 and 48 h after exercise.

Results

At 1-h post-exercise, phosphorylation of p70S6K^Thr389 and p38MAPK^{Thr180/Tyr182} was elevated in the BFR leg, but not in the free-flow leg. Phospho-p70S6K^Thr389 was elevated three- to fourfold in both legs at 24-h post-exercise, but back to baseline at 48 h. The number of visible satellite cells (SCs) per muscle fibre was increased for all post-exercise time points and in both legs (33–53 %). The proportion of SCs with cytoplasmic extensions was elevated at 1-h post in the BFR leg and the number of SCs positive for myogenin and/or MyoD was increased at 1- and 24-h post-exercise for both legs combined.

Conclusion

Acute low-load resistance exercise with BFR resulted in early (1 h) and late (24 h) enhancement of phospho-p70S6K^Thr389, an early response of p38MAPK, and an increased number of SCs per muscle fibre. Enhanced phospho-p70S6K^Thr389 at 24-h post-exercise and increases in SC numbers were seen also in the free-flow leg. Implications of these findings for the hypertrophic effects of fatiguing low-load resistance exercise with and without BFR are discussed.

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Titel: Acute low-load resistance exercise with and without blood flow restriction increased protein signalling and number of satellite cells in human skeletal muscle
verfasst von: Mathias Wernbom
William Apro
Gøran Paulsen
Tormod S. Nilsen
Eva Blomstrand
Truls Raastad
Publikationsdatum: 01.12.2013
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Applied Physiology / Ausgabe 12/2013
Print ISSN: 1439-6319
Elektronische ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-013-2733-5

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