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01.12.2017 | Research article | Ausgabe 1/2017 Open Access

BMC Cancer 1/2017

Amplification and up-regulation of MIR30D was associated with disease progression of cervical squamous cell carcinomas

Zeitschrift:
BMC Cancer > Ausgabe 1/2017
Autoren:
You Zhou, Yinghua Hao, Yuxia Li, Ruizhen Li, Ruifang Wu, Shubin Wang, Zhengyu Fang
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​s12885-017-3201-0) contains supplementary material, which is available to authorized users.

Abstract

Background

Cervical squamous cell carcinoma (CSCC) is the most frequent type among cervical cancers. Although the altered miRNA miR-30d expression and the amplified chromosome locus of MIR30D, 8q24, have been reported in somatic cancers, the definitive functional impact of such region especially in CSCC remains under-investigated.

Methods

One hundred thirty-six cases of CSCC tissues and matched adjacent normal ovarian epithelial tissues were assessed in this study. FISH and qPCR were performed to detect the copy number and microRNA expression of MIR30D gene in the collected samples. In in-vitro study, proliferation of CSCC cells were analyzed using WST-1 assay and invasion abilities of CSCC cells were evaluated by transwell assay. In-vivo study using a model of nude mice bearing tumor was also performed.

Results

Copy number gains of MIR30D were detected in 22.8% (31 out of 136) of CSCC samples. Copy number of MIR30D was positively correlated with tumor progression. CSCCs with lymph node metastases (LNM) also showed more frequencies (36.4%) of MIR30D amplification than those without LNM (18.4%, p < 0.05). CSCCs with increased copy number of MIR30D also showed a positive correlation with miR-30d up-regulation. Inhibition of miR-30d in CSCC cells led to impaired tumor growth and migration.

Conclusions

Copy number amplifications of MIR30D gene and enhanced expression of miR-30d were positively correlated with tumor progression in CSCCs, indicating miR-30d might play an oncomiric role in the progression of CSCC.
Zusatzmaterial
Additional file 1: Down-regulated genes in HeLa cells by mir-30d mimic transfection. Over-expression of miR-30d down-regulated 464 genes in HeLa cells. (XLS 76 kb)
12885_2017_3201_MOESM1_ESM.xls
Additional file 2: Down-regulated genes in HeLa cells by mir-30d mimic transfection. Over-expression of miR-30d down-regulated 376 genes in SiHa cells. (XLS 64 kb)
12885_2017_3201_MOESM2_ESM.xls
Additional file 3: List of the shared downregulated genes by miR-30d mimic transfection in Hela and SiHa cell lines, as well as the TargetScan-predicted miR-30d targets. One hundred twenty-nine down-regulated genes were shared in Hela and SiHa cell lines, and 68 of these were TargetScan predicted targets of miR-30d. (XLS 30 kb)
12885_2017_3201_MOESM3_ESM.xls
Literatur
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