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Journal of Cancer Research and Clinical Oncology

Erschienen in:

01.01.2012 | Original Paper

Analysis of the contribution of nasopharyngeal epithelial cancer cells to the induction of a local inflammatory response

verfasst von: Qianjin Liao, Xiaofang Guo, Xiaoling Li, Xiayu Li, Pan Chen, Fang Liang, Hailin Tang, Min Deng, Minghua Wu, Jian Ma, Wei Xiong, Guiyuan Li

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 1/2012

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Abstract

Purpose

We investigated the local contribution of nasopharyngeal epithelial cancer cells to the inflammatory process.

Materials and methods

THP-1 monocytes were treated with phorbol 12-myristate 13-acetate to induce the production of differentiated macrophages (D-THP-1), which were subsequently activated by lipopolysaccharide (LPS) (10 ng/ml). The production of pro-inflammatory cytokines in D-THP-1 cells was detected by ELISA and qRT-PCR. The effects of conditioned media harvested from LPS-treated D-THP-1 cells were investigated with regard to cell proliferation (MTT), production of pro-inflammatory cytokines (ELISA) and activation of NF-κB and STAT3 (western blot) in the nasopharyngeal epithelial cancer cell line 5–8F.

Results

LPS induced the production of the pro-inflammatory cytokines TNF-α (875.1 ± 68.31 pg/ml), IL-6 (42.2 ± 5.32 pg/ml), IL-1β (9.6 ± 1.34 pg/ml) and IL-8 (19.3 ± 3.47 pg/ml) in D-THP-1 cells significantly (P < 0.001) with similar results detected at the mRNA level. Exposure of 5–8F cells to conditioned medium from LPS-treated D-THP-1 cells significantly induced production of TNF-α (632.3 ± 71.32 pg/ml), IL-6 (51.3 ± 3.57 pg/ml), IL-1β (7.3 ± 1.31 pg/ml) and IL-8 (20.1 ± 2.36 pg/ml) (P < 0.01) and triggered significant activation of NF-κB and STAT3, which correlated with a concomitant degradation of IκBα and an increase in JAK2 phosphorylation (P < 0.05). Moreover, the LPS-treated D-THP-1 conditioned media promoted the proliferation of 5–8F cells (P < 0.05).

Conclusions

Nasopharyngeal epithelial cancer cells may play a significant role in maintaining and amplifying the inflammation process via activation of NF-κB and STAT3 pathway and through the local production of pro-inflammatory cytokines, which recruit and activate additional immune cells in the nasopharyngeal path and promote tumour progression.

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Titel: Analysis of the contribution of nasopharyngeal epithelial cancer cells to the induction of a local inflammatory response
verfasst von: Qianjin Liao
Xiaofang Guo
Xiaoling Li
Xiayu Li
Pan Chen
Fang Liang
Hailin Tang
Min Deng
Minghua Wu
Jian Ma
Wei Xiong
Guiyuan Li
Publikationsdatum: 01.01.2012
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 1/2012
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-011-1066-1

Springer Medizin

Analysis of the contribution of nasopharyngeal epithelial cancer cells to the induction of a local inflammatory response