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01.04.2016 | Natural Resource Letter

Anti-miroestrol polyclonal antibodies: a comparison of immunogen preparations used to obtain desired antibody properties

verfasst von: Tharita Kitisripanya, Kamonthip Jutathis, Chadathorn Inyai, Jukrapun Komaikul, Orapin Udomsin, Gorawit Yusakul, Hiroyuki Tanaka, Waraporn Putalun

Erschienen in: Journal of Natural Medicines | Ausgabe 2/2016

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Abstract

Immunogen quality is one important factor that contributes to desirable antibody characteristics. Highly specific antibodies against miroestrol can be used to develop a quality control immunoassay for Pueraria candollei products. In this study, we investigated how various immunogen preparations affect antibody properties. The results show that immunogen prepared using the Mannich reaction provides antibodies with higher specificity and sensitivity against miroestrol than immunogen prepared with the periodate reaction. The results suggest the Mannich reaction maintains the original structure of miroestrol and generates useful antibodies for developing immunoassays.

Cain JC (1960) Miroestrol: an oestrogen from the plant Pueraria mirifica. Nature 188:774–777CrossRefPubMed

Chansakaow S, Ishikawa T, Sekine K, Okada M, Higuchi Y, Kudo M, Chaichantipyuth C (2000) Isoflavonoids from Pueraria mirifica and their estrogenic activity. Planta Med 66(6):572–575CrossRefPubMed

Udomsuk L, Juengwattanatrakul T, Jarukamjorn K, Putalun W (2012) Increased miroestrol, deoxymiroestrol and isoflavonoid accumulation in callus and cell suspension cultures of Pueraria candollei var. mirifica. Acta Physiol Plant 34(3):1093–1100CrossRef

Shimokawa S, Kumamoto T, Ishikawa T, Takashi M, Higuchi Y, Chaichantipyuth C, Chansakaow S (2013) Quantitative analysis of miroestrol and kwakhurin for standardisation of Thai miracle herb ‘Kwao Keur’ (Pueraria mirifica) and establishment of simple isolation procedure for highly estrogenic miroestrol and deoxymiroestrol. Nat Prod Res 27(4–5):371–378CrossRefPubMed

Yusakul G, Putalun W, Udomsin O, Juengwatanatrakul T, Chaichantipyuth C (2011) Comparative analysis of the chemical constituents of two varieties of Pueraria candollei. Fitoterapia 82(2):203–207CrossRefPubMed

Yusakul G, Udomsin O, Juengwatanatrakul T, Tanaka H, Chaichantipyuth C, Putalun W (2013) High performance enzyme-linked immunosorbent assay for determination of miroestrol, a potent phytoestrogen from Pueraria candollei. Anal Chim Acta 785:104–110CrossRefPubMed

Chansakaow S, Ishikawa T, Seki H, Sekine (née Yoshizawa) K, Okada M, Chaichantipyuth C (2000b) Identification of deoxymiroestrol as the actual rejuvenating principle of “Kwao Keur”, Pueraria mirifica. The known miroestrol may be an artifact. J Nat Prod 63:173–175

Feng Y, Zhou Y, Zou Q, Wang J, Chen F, Gao Z (2009) Preparation and characterization of bisphenol A-cationized bovine serum albumin. J Immunol Methods 340:138–143CrossRefPubMed

Muckerheide A, Apple RJ, Pesce AJ, Michael JG (1987) Cationization of protein antigens, I. Alteration of immunogenic properties. J Immunol 138:833–837PubMed

10.

Marco M, Gee S, Hammock BD (1995) Immunochemical techniques for environmental analysis II. Antibody production and immunoassay development. Trends Anal Chem 14:415–425

11.

Singh KV, Kaur J, Varshney GC, Raje M, Suri CR (2004) Synthesis and characterization of hapten protein conjugates for antibody production against small molecules. Bioconjugate Chem 15(1):168–173CrossRef

Titel: Anti-miroestrol polyclonal antibodies: a comparison of immunogen preparations used to obtain desired antibody properties
verfasst von: Tharita Kitisripanya
Kamonthip Jutathis
Chadathorn Inyai
Jukrapun Komaikul
Orapin Udomsin
Gorawit Yusakul
Hiroyuki Tanaka
Waraporn Putalun
Publikationsdatum: 01.04.2016
Verlag: Springer Japan
Erschienen in: Journal of Natural Medicines / Ausgabe 2/2016
Print ISSN: 1340-3443
Elektronische ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-015-0949-x

Springer Medizin

Abstract

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