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01.12.2012 | Research | Ausgabe 1/2012 Open Access

Malaria Journal 1/2012

Anti-plasmodial and insecticidal activities of the essential oils of aromatic plants growing in the Mediterranean area

Zeitschrift:
Malaria Journal > Ausgabe 1/2012
Autoren:
Mario Dell’Agli, Cinzia Sanna, Patrizia Rubiolo, Nicoletta Basilico, Elisa Colombo, Maria M Scaltrito, Mamadou Ousmane Ndiath, Luca Maccarone, Donatella Taramelli, Carlo Bicchi, Mauro Ballero, Enrica Bosisio
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1475-2875-11-219) contains supplementary material, which is available to authorized users.

Competing interest

The authors declare that they have no competing interests.

Authors’ contributions

MDA, CS and MB designed the research. CS and MB supplied the plant material and EOs. EC, NB, MMS and DT performed the experiments for the anti-plasmodial activity. PT, LM and CB performed the analyses of EO composition. MON was responsible for the larvicidal and insecticidal experiments. MDA and EB drafted and wrote the final manuscript. All authors approved the final manuscript.

Abstract

Background

Sardinia is a Mediterranean area endemic for malaria up to the last century. During a screening study to evaluate the anti-plasmodial activity of some aromatic plants traditionally used in Sardinia, Myrtus communis (myrtle, Myrtaceae), Satureja thymbra (savory, Lamiaceae), and Thymus herba-barona (caraway thyme, Lamiaceae) were collected in three vegetative periods: before, during and after flowering.

Methods

The essential oils were obtained by steam distillation, fractionated by silica gel column chromatography and analysed by GC-FID-MS. Total oil and three main fractions were tested on D10 and W2 strains of Plasmodium falciparum in vitro. Larvicidal and adulticidal activities were tested on Anopheles gambiae susceptible strains.

Results

The essential oil of savory, rich in thymol, was the most effective against P. falciparum with an inhibitory activity independent from the time of collection (IC50 17–26 μg/ml on D10 and 9–11 μg/ml on W2). Upon fractionation, fraction 1 was enriched in mono-sesquiterpenoid hydrocarbons; fraction 2 in thymol (73-83%); and fraction 3 contained thymol, carvacrol and terpinen-4-ol, with a different composition depending on the time of collection. Thymol-enriched fractions were the most active on both strains (IC50 20–22 μg/ml on D10 and 8–10 μg/ml on W2) and thymol was confirmed as mainly responsible for this activity (IC50 19.7± 3.0 and 10.6 ± 2.0 μg/ml on D10 and W2, respectively). The essential oil of S. thymbra L. showed also larvicidal and adulticidal activities. The larvicidal activity, expressed as LC50, was 0.15 ± 0.002; 0.21 ± 0.13; and 0.15 ± 0.09 μg/ml (mean ± sd) depending on the time of collection: before, during and after flowering, respectively.

Conclusions

This study provides evidence for the use of essential oils for treating malaria and fighting the vector at both the larval and adult stages. These findings open the possibility for further investigation aimed at the isolation of natural products with anti-parasitic properties.
Zusatzmaterial
Authors’ original file for figure 1
12936_2012_2170_MOESM1_ESM.pdf
Authors’ original file for figure 2
12936_2012_2170_MOESM2_ESM.pdf
Authors’ original file for figure 3
12936_2012_2170_MOESM3_ESM.pdf
Literatur
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