Background
Materials and methods
Sample preparation and extraction procedure
Determination of some bioactive compound content of onions extracts
Determination of antioxidant properties of onions extracts
Evaluation of in vivo analgesic and anti-inflammatory activities of onion extract
Experimental animals and treatments
Anti-inflammatory test
Groups | Experimental design (rat paw edema) | Experimental design (whriting test) |
---|---|---|
Group 1 | Negative control | Negative control |
Group 2 | Celecoxib (1 mg/Kg) | Diclofenac (1 mg/Kg) |
Group 3 | Dexamethasome (1 mg/kg) | / |
Group 4 | Violet of galmi (0.1 mg/Kg) | Violet of galmi (0.1 mg/Kg) |
Group 5 | Violet of galmi (0.5 mg/Kg) | Violet of galmi (0.5 mg/Kg) |
Group 6 | Violet of galmi (1.5 mg/Kg) | Violet of galmi (1.5 mg/Kg) |
Group 7 | Goudami (0.1 mg/Kg) | Goudami (0.1 mg/Kg) |
Group 8 | Goudami (0.5 mg/Kg) | Goudami (0.5 mg/Kg) |
Group 9 | Goudami (1.5 mg/Kg) | Goudami (1.5 mg/Kg) |
Analgesic property
Statistical analysis
Results
Polyphenol content and antioxidant properties of onion extracts
Parameters (%) | Onion variety | |
---|---|---|
Violet de galmi | Goudami | |
Total phenolics | 67.43 ± 1.64b | 58.03 ± 1.52a |
Flavonoids | 38.12 ± 0.31b | 24.11 ± 1.12a |
Tannins | 7.21 ± 0.15a | 17.13 ± 2.23b |
Vit C | 4.13 ± 0.16a | 6.02 ± 0.15b |
Concentration (mg/mL) | Onion variety | Ascorbic acid | |
---|---|---|---|
Violet de galmi | Goudami | ||
0.1 | 0.37 ± 0.04b | 0.27 ± 0.03a | 0.48 ± 0.01c |
0.5 | 0.48 ± 0.06b | 0.35 ± 0.04 a | 0.69 ± 0.02c |
1 | 0.87 ± 0.07 b | 0.65 ± 0.15a | 0.98 ± 0.02c |
1.5 | 0.94 ± 0.11b | 0.71 ± 0.05a | 1.08 ± 0.02c |
Concentration (mg/mL) | Onion variety | Trolox | |
---|---|---|---|
Violet de galmi | Goudami | ||
0.1 | 45.51 ± 0.11b | 37.33 ± 2.14a | 86.62 ± 1.42c |
0.5 | 124.67 ± 2.01b | 63.72 ± 1.11a2 | 153.71 ± 2.04c |
1 | 188.12 ± 1.21b | 147.17 ± 3.82a | 289.24 ± 3.22c |
1.5 | 202.08 ± 1.01b | 165.11 ± 2.14a | 312.01 ± 2.13c |
Concentration (mg/mL) | Onion variety | EDTA | |
---|---|---|---|
Violet de galmi | Goudami | ||
0.1 | 23.15 ± 1.31 a | 47.16 ± 2.14 b | 47.51 ± 1.12 c |
0.5 | 47.12 ± 2.42 a | 74.25 ± 1.32 b | 84.76 ± 2.03 c |
1 | 73.55 ± 1.23 a | 96.51 ± 2.22 b | 97.75 ± 1.12 b |
1.5 | 74.58 ± 2.15 a | 97.42 ± 1.67 b | 99.08 ± 1.21 b |
Anti-inflammatory properties of onion extracts
Treatments | Inhibition percentage (%) at different time | ||||
---|---|---|---|---|---|
30 min | 60 min | 120 min | 180 min | 350 min | |
Negative control | 59.52 ± 0.86a | 65.07 ± 1.16a | 65.07 ± 1.15a | 72.98 ± 1.72b | 77.18 ± 1.99b |
Celecoxib (1 mg/kg) | 81.58 ± 1.67b | 83.47 ± 1.78c | 83.47 ± 1.45c | 86.30 ± 1.76c | 93.84 ± 1.23c |
Dexamethasome (1 mg/kg) | 93.44 ± 1.18e | 100 ± 0.15e | 100 ± 0.86e | 100 ± 0.11e | 100 ± 0.57e |
V.Galmi (0.1 mg/kg) | 82.60 ± 1.39b | 69.47 ± 1.19b | 69.47 ± 1.99b | 70.87 ± 1.01b | 71.32 ± 1.39a |
V.Galmi (0.5 mg/kg) | 89.20 ± 1.48d | 91.56 ± 1.58d | 91.56 ± 0.80d | 100 ± 0.25e | 100 ± 0.51e |
V.Galmi (1.5 mg/kg) | 94.46 ± 1.41e | 100 ± 0.52e | 100 ± 0.41e | 100 ± 0.22e | 100 ± 0.57e |
Goudami (0.1 mg/kg) | 78.96 ± 1.54b | 63.54 ± 0.29a | 63.54 ± 1.22a | 61.66 ± 1.77a | 71.94 ± 1.91a |
Goudami (0.5 mg/kg) | 81.52 ± 1.24b | 81.99 ± 1.72c | 81.99 ± 1.16c | 95.37 ± 1.14d | 98.15 ± 0.80d |
Goudami (1.5 mg/kg) | 85.03 ± 0.95c | 91.57 ± 1.52d | 91.57 ± 1.31d | 100 ± 0.42e | 100 ± 0.11e |
Analgesic property of onion extract
Principal Component Analysis (PCA) of the properties of onion extracts
Variables | Total Reducing P. | DPPH scavenging | Chelating P. | Edema Inhibition | number Writhes | Paw edema volume | Flavonoids | Tannins | Vit C | Total Phenolic C. |
---|---|---|---|---|---|---|---|---|---|---|
Total Reducing P | 1 | |||||||||
DPPH scavenging | 0.74 | 1 | ||||||||
Chelating Power | 0.54 | 0.77 | 1 | |||||||
Edema Inhibition | 0.75 | 0.97 | 0.82 | 1 | ||||||
number Writhes | −0.81 | −0.76 | −0.41 | −0.72 | 1 | |||||
Paw edema volume | −0.75 | −0.97 | −0.81 | −0.99 | 0.71 | 1 | ||||
Flavonoids | 0.81 | 0.96 | 0.65 | 0.89 | −0.89 | − 0.88 | 1 | |||
Tannins | 0.68 | 0.61 | 0.89 | 0.72 | −0.44 | −0.72 | 0.52 | 1 | ||
Vit C | 0.80 | 0.81 | 0.92 | 0.87 | −0.64 | −0.86 | 0.75 | 0.96 | 1 | |
Total Phenolic C. | 0.84 | 0.97 | 0.72 | 0.92 | −0.88 | −0.91 | 0.99 | 0.63 | 0.83 | 1 |