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Forensic Science, Medicine and Pathology
Erschienen in: Forensic Science, Medicine and Pathology 1/2023

08.11.2022 | Original Article

Application of natural sciences methodology in archaeological study of Iron Age burials in Latvia: pilot study

verfasst von: Janis Kimsis, Elina Petersone-Gordina, Alise Poksane, Antonija Vilcāne, Joanna Moore, Guntis Gerhards, Renate Ranka

Erschienen in: Forensic Science, Medicine and Pathology | Ausgabe 1/2023

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Abstract

Natural sciences provide several modern methodologies that could be successfully applied in archaeological studies. In this pilot study, archaeological human remains from two Iron Age cemeteries (7th–twelfth centuries AD), Lejasbitēni and Čunkāni-Dreņģeri, which are located in different regions of Latvia, were studied. We applied ancient DNA (aDNA) and tooth enamel peptide analysis to determine the biological sex of the individuals. In addition, aDNA analysis was used to perform mtDNA haplogroup analysis. In most cases, the results of aDNA analysis regarding the biological sex of individuals coincided with the gender assigned based on grave orientation and grave goods. The results of sex determination using peptide analysis in all four individuals for whom data were available matched the possible gender. Of the 17 samples that had sufficient DNA for sequencing, seven samples had enough reads to perform mtDNA haplogroup analysis. The H2a2a, I4a1, H2a2a1, and H16c mtDNA haplogroups were identified in the individuals from the Lejasbitēni cemetery, while the T2b and K1a + 150 mtDNA haplogroups were identified in the individuals from the Čunkāni-Dreņģeri cemetery. Overall, the obtained results demonstrated the feasibility of applying aDNA and tooth enamel peptide analysis for biological sex determination within archaeological studies. The availability of human aDNA data will be highly useful for investigating the demographic history and social structures in Iron Age Latvia.
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Literatur
1.
Bruzek J. A method for visual determination of sex, using the human hip bone. Am J Phys Anthropol. 2002;117:157–68.CrossRefPubMed
2.
White TD, Black MT, Folkens PA. Human osteology. 3rd ed. San Diego, CA: Academic Press; 2011.
3.
Cox M. Ageing adults from the skeleton. In M. Cox and S. Mays (eds), Human Osteology in Archaeology and Forensic Science, London; 2000. pp. 61–82.
4.
Lewis M. The Bioarchaeology of Children: Perspectives from Biological and Forensic Anthropology. Cambridge, UK: Cambridge Univ Press; 2007.
5.
Walker PL, Cook DC. Brief communication: gender and sex: vive la difference. Am J Phys Anthropol. 1998;106:255–9.CrossRefPubMed
6.
Bauer CM, Niederstätter H, McGlynn G, Stadler H, Parsona W. Comparison of morphological and molecular genetic sex-typing on mediaeval human skeletal remains. Forensic Sci Int Genet. 2013;7:581–6.CrossRefPubMedPubMedCentral
7.
Schmidt D, Hummel S, Herrmann B. Brief communication: multiplex X/Y-PCR improves sex identification in aDNA analysis. Am J Phys Anthropology. 2003;121:337–42.CrossRef
8.
Gibbon V, Paximadis M, Strkalj G, Ruff P, Penny C. Novel methods of molecular sex identification from skeletal tissue using the amelogenin gene. Forensis Sci Int Genet. 2009;3:74–9.CrossRef
9.
Bollongino R, Tresset A, Vigne J-D. Environment and excavation: pre-lab impacts on ancient DNA analyses. CR Palevol. 2008;7:91–8.CrossRef
10.
Masuyama K, Shojo H, Nakanishi H, Inokuchi S, Adachi N. Sex Determination from Fragmented and Degenerated DNA by Amplified Product-Length Polymorphism Bidirectional SNP Analysis of Amelogenin and SRY Genes. PLoS ONE. 2017;12(1): e0169348.CrossRefPubMedPubMedCentral
11.
Skoglund P, Storå J, Götherström A, Jakobsson M. Accurate sex identification of ancient human remains using DNA shotgun sequencing. J Archaeol Sci. 2013;40:4477–82.CrossRef
12.
Knipper C, Mittnik A, Massy K, Kociumaka C, Kucukkalipci I, Maus M, Wittenborn F, Metz SE, Staskiewicz A, Krause J, Stockhammer PW. Female exogamy and gene pool diversification at the transition from the Final Neolithic to the Early Bronze Age in central Europe. Proc Natl Acad Sci USA. 2017;114:10083–8.CrossRefPubMedPubMedCentral
13.
Mittnik A, Wang C-C, Svoboda J, Krause J. A molecular approach to the sexing of the triple burial at the upper paleolithic site of Dolní Věstonice. PLoS ONE. 2016;11(10): e0163019.CrossRefPubMedPubMedCentral
14.
Stewart NA, Gerlach RF, Gowland RL, Gron KJ, Montgomery J. Sex determination of human remains from peptides in tooth enamel. Proc Natl Acad Sci USA. 2017;114:13649–54.CrossRefPubMedPubMedCentral
15.
Gowland R, Stewart NA, Crowder KD, Hodson C, Shaw H, Gron KJ, Montgomery J. Sex estimation of teeth at different developmental stages using dimorphic enamel peptide analysis. Am J Phys Anthropol. 2021;174:859–69.CrossRefPubMed
16.
Merheb M, Matar R, Hodeify R, Siddiqui SS, Vazhappilly CG, Marton J, Azharuddin S, Al ZH. Mitochondrial DNA, a powerful tool to decipher ancient human civilization from domestication to music, and to uncover historical murder cases. Cells. 2019;8:433.CrossRefPubMedPubMedCentral
17.
Översti S, Majander K, Salmela E, Salo K, Arppe L, Belskiy S, Etu-Sihvola H, Laakso V, Mikkola E, Pfrengle S, Putkonen M, Taavitsainen JP, Vuoristo K, Wessman A, Sajantila A, Oinonen M, Haak W, Schuenemann VJ, Krause J, Palo JU, Onkamo P. Human mitochondrial DNA lineages in Iron-Age Fennoscandia suggest incipient admixture and eastern introduction of farming-related maternal ancestry. Sci Rep. 2019;9:16883.CrossRefPubMedPubMedCentral
18.
Vasks A. The pre-history of Latvia (10500 BCE - 1200 CE). Latvia and Latvians: Collection of scholarly articles, Vol. 2, Part 3. Rīga: Latvian Academy of Sciences; 2018. pp. 9–39.
19.
20.
Atgāzis, M. Vidējais dzelzs laikmets, 400.-800.g. [Middle Iron Age, 400–800 ADCE]. Latvijas senākā vēsture. Rīga: Latvijas vēstures institūta apgāds; 2001.
21.
Urtāns V. Etniskās atšķirības apbedīšanas tradīcijās un kapu inventārā 5. - 9. gs [Ethnic differences in burial traditions and grave inventories in the 5th - 9th centuries AD]. Arheoloģija un etnogrāfija, 9. laidiens. Rīga; 1970. pp 105–125, 61–83.
22.
Atgāzis M. Dreņģeru-Čunkānu kapulauks un zemgaļu senvēstures pētniecības jautājumi [Dreņģeri-Čunkāni cemetery and the history of ancient Semigallian culture]. Zinātniskās atskaites sesijas materiāli par arheologu 1992. un 1993. gada pētījumu rezultātiem. Riga: Zinātne; 1994. pp. 23–30.
23.
Buikstra JE, Ubelaker DH. Standards for data collection from human skeletal remains. In J. E. Buikstra & D. H. Ubelaker (Eds.), Fayetteville: Arkansas Archeological Survey Research Series No 44; 1994.
24.
AlQahtani SJ, Hector M, Liversidge H. Brief communication: the London atlas of human tooth development and eruption. Am J Phys Anthropol. 2010;142:481–90.CrossRefPubMed
25.
Šnē A. Sabiedrība un vara: Sociālās attiecības Austrumlatvijā aizvēstures beigās [Society and power: social relations in eastern Latvia in the end of prehistory]. Rīga: Intelekts; 2002.
26.
Keyser-Tracqui C, Ludes B. Methods for the study of ancient DNA. Methods Mol Biol. 2005;297:253–64.PubMed
27.
28.
Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: Summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016;32:3047–8.CrossRefPubMedPubMedCentral
29.
30.
31.
Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R. 1000 Genome project data processing subgroup author notes. The sequence alignment/map format and SAMtools. Bioinformatics. 2009;25:2078–9.
32.
33.
Jónsson H, Ginolhac A, Schubert M, Johnson P, Orlando L. mapDamage2.0: fast approximate Bayesian estimates of ancient DNA damage parameters. Bioinformatics. 2013;29:1682–4.
34.
Weissensteiner H, Pacher D, Kloss-Brandstätter A, Forer L, Specht G, Bandelt H, Kronenberg F, Salas A, Schönherr S. HaploGrep 2: mitochondrial haplogroup classification in the era of high-throughput sequencing. Nucleic Acids Res. 2016;44:W58-63.CrossRefPubMedPubMedCentral
35.
Lewis M. The bioarchaeology of children: Perspective from biological and forensic anthropology. Cambridge: Cambridge University Press; 2007.
36.
Scheuer L, Black S. Developmental juvenile osteology. In L. Scheuer & S. B. T.-D. J. O. Black (Eds.). Cambridge, MA: Academic Press; 2000.
37.
Işcan MY, Derrick K. Determination of sex from the sacroiliac joint: a visual assessment technique. Florida scientist. 1984;47:94–8.
38.
Phenice TW. A newly developed visual method of sexing the os pubis. Am J Phys Anthropol. 1969;30:297–301.CrossRefPubMed
39.
Walker PL. Problems of preservation and sexism in sexing: some lessons from historical collections for palaeodemographers. In: Herring A, Saunders SR, editors. Grave Reflections: Portraying the Past through Cemetery Studies. Toronto: Canadian Scholars’ Press; 1995. p. 31–44.
40.
Radiņš A. 10. –13. gadsimta senkapi latgaļu apdzīvotajā teritorijā un Austrumlatvijas etniskās, sociālās un politiskās vēstures jautājumi [Ancient cemeteries from the 10th – 13th centuries AD in the Latgalian territories, and the ethnic, social, and political issues of the eastern Latvia]. Latvijas Vēstures Muzeja raksti Nr. 5: Arheoloģija. Rīga: N.I.M.S.; 1999. pp. 25–7.
41.
Stratton S. “Seek and you shall find.” How the analysis of gendered patterns in archaeology can create false binaries: a case study from Durankulak. J Archaeol Method Th. 2016;23:854–69.
42.
Afonso C, Nociarova D, Santos C, Martinez-Labarga C. Sex selection in late Iberian infant burials: Integrating evidence from morphological and genetic data. Am J Hum Biol. 2019;31: e23204.CrossRefPubMed
43.
44.
Stone AC, Milner GR, Pääbo S, Stoneking M. Sex determination of ancient human skeletons using DNA. Am J Phys Anthropol. 1996;99:231–8.CrossRefPubMed
45.
Key FM, Posth C, Krause J, Herbig A, Bos KI. Mining Metagenomic Data Sets for Ancient DNA: Recommended Protocols for Authentication. Trends Genet. 2017;33:508–20.CrossRefPubMed
46.
47.
Hansen HB, Damgaard PB, Margaryan A, Stenderup J, Lynnerup N, Willerslev E, Allentoft ME. Comparing ancient DNA preservation in petrous bone and tooth cementum. PLoS ONE. 2017;12: e0170940.CrossRefPubMedPubMedCentral
48.
Damgaard PB, Margaryan A, Schroeder H, Orlando L, Willerslev E, Allentoft ME. Improving access to endogenous DNA in ancient bones and teeth. Sci Rep. 2015;5:11184.CrossRefPubMedPubMedCentral
49.
Buonasera T, Eerkens J, de Flamingh A, Engbring L, Yip J, Li H, Haas R, DiGiuseppe D, Grant D, Salemi M, Nijmeh C, Arellano M, Leventhal A, Phinney B, Byrd BF, Malhi RS, Parker G. A comparison of proteomic, genomic, and osteological methods of archaeological sex estimation. Sci Rep. 2020;10(1):11897.CrossRefPubMedPubMedCentral
50.
Parker GJ, Yip JM, Eerkens JW, Salemi M, Durbin-Johnson B, Kiesow C, Haas R, Buikstra JE, Klaus H, Regan LA, Rocke DM, Phinney BS. Sex estimation using sexually dimorphic amelogenin protein fragments in human enamel. J Archaeol Sci. 2019;101:169–80.CrossRef
Metadaten
Titel
Application of natural sciences methodology in archaeological study of Iron Age burials in Latvia: pilot study
verfasst von
Janis Kimsis
Elina Petersone-Gordina
Alise Poksane
Antonija Vilcāne
Joanna Moore
Guntis Gerhards
Renate Ranka
Publikationsdatum
08.11.2022
Verlag
Springer US
Erschienen in
Forensic Science, Medicine and Pathology / Ausgabe 1/2023
Print ISSN: 1547-769X
Elektronische ISSN: 1556-2891
DOI
https://doi.org/10.1007/s12024-022-00553-7

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