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Virchows Archiv

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01.04.2008 | Original Article

Augmented β cell loss and mitochondrial abnormalities in sucrose-fed GK rats

verfasst von: Hiroki Mizukami, Ryuichi Wada, Motoi Koyama, Teruko Takeo, Sechiko Suga, Makoto Wakui, Soroku Yagihashi

Erschienen in: Virchows Archiv | Ausgabe 4/2008

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Abstract

Progressive decline of islet β cell mass is a hallmark of type 2 diabetes, where nutritional insults are invoked in the pathologic process. Its detailed mechanisms are, however, incompletely understood. We explored the effect of sucrose diet on mitochondria in Goto Kakizaki (GK) rats, a spontaneously diabetic model. Six-week-old male GK rats were given 30% sucrose orally for 2 weeks. Normal Wistar rats fed with sucrose served as controls. Compared to untreated GK rats, sucrose-fed GK rats showed severe degeneration and death of β cells with disrupted and swollen mitochondria and a greater β cell loss. Submicroscopic analysis disclosed a smaller mean volume and a greater number of mitochondria in β cells in GK rats compared to those in Wistar rats. Mitochondria in sucrose-fed GK rats were 2.4-fold greater in mean volume than those in untreated state. Without sucrose feeding, there was no significant difference in mitochondrial membrane potentials (MmPs) of isolated islets between Wistar and GK rats. MmPs were reduced by 44% in sucrose-fed GK rats but not influenced in sucrose-fed Wistar rats. Current results suggest that nutritional insults like sucrose feeding may exert deleterious effects on mitochondria, resulting in augmented β cell loss in type 2 diabetes.

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Titel: Augmented β cell loss and mitochondrial abnormalities in sucrose-fed GK rats
verfasst von: Hiroki Mizukami
Ryuichi Wada
Motoi Koyama
Teruko Takeo
Sechiko Suga
Makoto Wakui
Soroku Yagihashi
Publikationsdatum: 01.04.2008
Verlag: Springer-Verlag
Erschienen in: Virchows Archiv / Ausgabe 4/2008
Print ISSN: 0945-6317
Elektronische ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-007-0508-2

Springer Medizin

Augmented β cell loss and mitochondrial abnormalities in sucrose-fed GK rats

Abstract

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

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