c-erbB2 receptor and P53 protein expression in canine mammary gland carcinoma

The objective of this study was to investigate the immunohistochemical (IHC) detection of c-erbB2 receptor (crbP) and TP53, the co-expression of these two markers and their relationship with histological type and histological grade in canine mammary carcinoma. After histological diagnosis according to the WHO classification of 30 mammary carcinomas, their histologic grade was identified by the Elston and Ellis method for human breast tumours. Proliferation activity was detected by Ki67 protein. IHC staining was carried out for crbP and TP53 expression. Results of semiquantitative IHC assessment of crbP and 3+ TP53 expression were 23.3% and 53.3% of canine mammary carcinoma, respectively. There were significant relationships between Tp53 and histopathological grade and TP53 and Pki67. No significant results were obtained between crbP and TP53 even with overexpression of TP53 in positive crbP cases. These results suggest that using crbP and TP53 may be useful for malignancy detection accompanied by Pki67, and additional research in this subject is essential.