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01.05.2010 | Materno-fetal Medicine

Carrier-mediated uptake of Levofloxacin by BeWo cells, a human trophoblast cell line

verfasst von: Hana Polachek, Gershon Holcberg, Joseph Polachek, Mazal Rubin, Valeria Feinshtein, Eyal Sheiner, Zvi Ben-Zvi

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 5/2010

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Abstract

Objective

Placental transfer of Levofloxacin (LF), a broad spectrum fluoroquinolone antibiotic, and its inhibition was investigated in BeWo cells, a human trophoblast cell line.

Methods

The experiments of LF uptake by BeWo cells were performed after preincubation and in the presence of the P-glycoprotein inhibitors (Cyclosporin A, Verapamil and Quercetin), the organic anion/cation transporter inhibitor (Cimetidine) and the MCT substrates (lactic acid and salicylic acid).

Results

P-glycoprotein inhibitors increased the uptake of LF by BeWo cells. The increase in LF accumulation by Cyclosporin A, Verapamil and Quercetin was by 30, 90 and 80%, respectively. Cimetidine, the organic cation inhibitor, increased the transport of LF by 48%. Lactic acid and salicylic acid, the MCT substrates, initially decreased the accumulation of LF by 30% and subsequently increased the uptake of LF by 500 and 53%, respectively.

Conclusions

The uptake of LF by human trophoblast cells is mediated by multiple transporters as well as passive diffusion.

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Titel: Carrier-mediated uptake of Levofloxacin by BeWo cells, a human trophoblast cell line
verfasst von: Hana Polachek
Gershon Holcberg
Joseph Polachek
Mazal Rubin
Valeria Feinshtein
Eyal Sheiner
Zvi Ben-Zvi
Publikationsdatum: 01.05.2010
Verlag: Springer-Verlag
Erschienen in: Archives of Gynecology and Obstetrics / Ausgabe 5/2010
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-009-1177-y

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Carrier-mediated uptake of Levofloxacin by BeWo cells, a human trophoblast cell line