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23.11.2016 | Original Article

Cell-type-specific expression of NFIX in the developing and adult cerebellum

verfasst von: James Fraser, Alexandra Essebier, Richard M. Gronostajski, Mikael Boden, Brandon J. Wainwright, Tracey J. Harvey, Michael Piper

Erschienen in: Brain Structure and Function | Ausgabe 5/2017

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Abstract

Transcription factors from the nuclear factor one (NFI) family have been shown to play a central role in regulating neural progenitor cell differentiation within the embryonic and post-natal brain. NFIA and NFIB, for instance, promote the differentiation and functional maturation of granule neurons within the cerebellum. Mice lacking Nfix exhibit delays in the development of neuronal and glial lineages within the cerebellum, but the cell-type-specific expression of this transcription factor remains undefined. Here, we examined the expression of NFIX, together with various cell-type-specific markers, within the developing and adult cerebellum using both chromogenic immunohistochemistry and co-immunofluorescence labelling and confocal microscopy. In embryos, NFIX was expressed by progenitor cells within the rhombic lip and ventricular zone. After birth, progenitor cells within the external granule layer, as well as migrating and mature granule neurons, expressed NFIX. Within the adult cerebellum, NFIX displayed a broad expression profile, and was evident within granule cells, Bergmann glia, and interneurons, but not within Purkinje neurons. Furthermore, transcriptomic profiling of cerebellar granule neuron progenitor cells showed that multiple splice variants of Nfix are expressed within this germinal zone of the post-natal brain. Collectively, these data suggest that NFIX plays a role in regulating progenitor cell biology within the embryonic and post-natal cerebellum, as well as an ongoing role within multiple neuronal and glial populations within the adult cerebellum.

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Titel: Cell-type-specific expression of NFIX in the developing and adult cerebellum
verfasst von: James Fraser
Alexandra Essebier
Richard M. Gronostajski
Mikael Boden
Brandon J. Wainwright
Tracey J. Harvey
Michael Piper
Publikationsdatum: 23.11.2016
Verlag: Springer Berlin Heidelberg
Erschienen in: Brain Structure and Function / Ausgabe 5/2017
Print ISSN: 1863-2653
Elektronische ISSN: 1863-2661
DOI: https://doi.org/10.1007/s00429-016-1340-8

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