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Archives of Virology

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13.10.2017 | Original Article

Characterization and genome comparison of an Indian isolate of bidensovirus infecting the silkworm Bombyx mori

verfasst von: Tania Gupta, Katsuhiko Ito, Keiko Kadono-Okuda, Geetha N. Murthy, E. Vijaya Gowri, Kangayam M. Ponnuvel

Erschienen in: Archives of Virology | Ausgabe 1/2018

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Abstract

The bipartite genome of an Indian isolate of Bombyx mori bidensovirus (BmBDV), one of the causative agents of the fatal silkworm disease ‘Flacherie’, was cloned and completely sequenced. Nucleotide sequence analysis of this Indian isolate of BmBDV revealed two viral DNA segments, VD1 and VD2 as well as a DNA polymerase motif which supports its taxonomical status as the type species of a new family of Bidnaviridae. The Indian isolate of BmBDV was found to have a total of six putative ORFs four of which were located on the VD1 with the other two being on the VD2 DNA segment. The VD1 DNA segment was found to code for three non-structural proteins including a viral DNA polymerase as well as one structural protein, while the VD2 DNA segment was found to code for one structural and one non-structural protein, similar to that of the Japanese and Zhenjiang isolates of BmBDV. A BmBDV ORF expression study was done through real time qPCR wherein the VD2 ORF 1 and 2 showed the maximum transcript levels. This is the first report of the genome characterization of an Indian isolate of BmBDV, infecting silkworm B. mori.

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Titel: Characterization and genome comparison of an Indian isolate of bidensovirus infecting the silkworm Bombyx mori
verfasst von: Tania Gupta
Katsuhiko Ito
Keiko Kadono-Okuda
Geetha N. Murthy
E. Vijaya Gowri
Kangayam M. Ponnuvel
Publikationsdatum: 13.10.2017
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 1/2018
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-017-3584-x

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