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Archives of Virology

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01.02.2016 | Brief Report

Characterization of siRNAs derived from cucumber green mottle mosaic virus in infected cucumber plants

verfasst von: Yongqiang Li, Congliang Deng, Qiaoxia Shang, Xiaoli Zhao, Xingliang Liu, Qi Zhou

Erschienen in: Archives of Virology | Ausgabe 2/2016

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Abstract

Virus-derived small interfering RNAs (vsiRNAs) of cucumber green mottle mosaic virus (CGMMV), a member of the genus Tobamovirus, were characterised in cucumber plants by deep sequencing. CGMMV vsiRNAs of 21-22 nt in length predominated, suggesting that there might be a conserved mechanism of DCL2 and DCL4 involvement in the biogenesis of vsiRNAs, as well as a common RNA silencing pathway in CGMMV-infected cucumber plants. The 5′-terminal base of vsiRNAs was biased towards C/A/U, suggesting that CGMMV vsiRNAs might be loaded into diverse AGO-containing RISCs to disturb the gene expression of host plants. Possible targets for some of the vsiRNAs were also predicted.

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Titel: Characterization of siRNAs derived from cucumber green mottle mosaic virus in infected cucumber plants
verfasst von: Yongqiang Li
Congliang Deng
Qiaoxia Shang
Xiaoli Zhao
Xingliang Liu
Qi Zhou
Publikationsdatum: 01.02.2016
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 2/2016
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-015-2687-5

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