Background
Methods
Participants
Clinical and neuropsychological assessment
Blood sampling
PiB-PET
Reagents
INNO-BIA plasma Aβ forms assay using xMAP technology
Proof-of-concept experiments
Gel electrophoresis
xMAP technology
Time-dependent alterations in plasma Aβ42 and Aβ40 levels
Effects of MPP on discrimination between subject groups
Statistics
Results
Demographic data of subjects
Group (total, n = 353) | |||
---|---|---|---|
Basic characteristic | PiB– (n = 253) | PiB+ (n = 100) |
P valuea
|
Global PiB deposition, mean ± SEM | 1.11 ± 0.004 | 1.91 ± 0.038 | <0.001 |
Clinical diagnosis, N/M/D | 187/50/16 | 28/29/43 | <0.001†
|
Gender, male/female | 95/158 | 38/62 | >0.05†
|
Age (years), mean ± SEM | 69.94 ± 0.5 | 73.00 ± 0.7 | <0.001 |
CDR, mean ± SEM | 0.15 ± 0.02 | 0.5 ± 0.04 | <0.001 |
MMSE z score,b mean ± SEM | –0.13 ± 0.07 | –1.91 ± 0.20 | <0.001 |
Education, mean ± SEM | 10.65 ± 0.3 | 10.82 ± 0.5 | >0.05 |
ApoE4-positive, n/N (%) | 39/253 (15%) | 57/100 (57%) | <0.001†
|
Proof-of-concept experiments for the effects of MPP on Aβ quantification
POC for the effects of MPP on the distinction among the subject groups
MPP-Aβs reflect the pathological load of Aβ in the brain
CN | MCI | ADD | ND | CI | PiB– | PiB+ | All | |
---|---|---|---|---|---|---|---|---|
(n = 215) | (n = 79) | (n = 59) | (n = 294) | (n = 138) | (n = 253) | (n = 100) | (n = 353) | |
MPP-Aβ42 |
P = 0.7982 | **P < 0.01 |
P = 0.3132 |
P = 0.9827 |
P = 0.1125 |
P = 0.9750 |
P = 0.7965 |
P = 0.1776 |
r = 0.0175 |
r = 0.3479 |
r = –0.1336 |
r = –0.0012 |
r = 0.1357 |
r = 0.0020 |
r = –0.0261 |
r = –0.0719 | |
MPP-Aβ40 | *P = 0.0301 | **P < 0.01 |
P = 0.6639 | ***P < 0.001 | **P < 0.01 |
P = 0.8155 |
P = 0.2436 | ****P < 0.0001 |
r = 0.1479 |
r = 0.2950 |
r = 0.0578 |
r = 0.2177 |
r = 0.2272 |
r = –0.0147 |
r = 0.1177 |
r = 0.2309 | |
MPP-Aβ42/40 |
#
P = 0.0940 |
P = 0.6395 |
P = 0.2868 | **P < 0.01 |
P = 0.6488 |
P = 0.7416 |
P = 0.4437 | ****P < 0.0001 |
r = –0.1145 |
r = 0.0535 |
r = –0.1410 |
r = –0.1654 |
r = –0.0391 |
r = –0.0208 |
r = –0.0775 |
r = –0.2280 |
Prediction of cerebral amyloid deposition based on MPP-Aβ42/40 ratio
Panel | AUC | Sensitivity (%) | Specificity (%) |
P value | Cutoff criterion | 95% CI of AUC |
---|---|---|---|---|---|---|
ND– vs ND+ | ||||||
MPP-Aβ42/40 | 0.639 | 71.8 | 51.5 | <0.0001 | >0.1871 | 0.559–0.719 |
MPP-Aβ42/40 + age + gender | 0.695 | 79.0 | 57.8 | <0.0001 | >0.1864 | 0.622–0.767 |
MPP-Aβ42/40 + age + gender + ApoE | 0.783 | 80.7 | 69.2 | <0.0001 | >0.1730 | 0.715–0.852 |
PiB– vs PiB+ | ||||||
MPP-Aβ42/40 | 0.668 | 71.0 | 52.2 | <0.0001 | >0.2731 | 0.605–0.731 |
MPP-Aβ42/40 + age + gender | 0.682 | 71.0 | 54.2 | <0.0001 | >0.2759 | 0.622–0.741 |
MPP-Aβ42/40 + age + gender + ApoE | 0.799 | 78.0 | 66.8 | <0.0001 | >0.2230 | 0.746–0.852 |