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Journal of Cardiovascular Translational Research

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12.09.2022 | Original Article

Collagen Molecular Damage is a Hallmark of Early Atherosclerosis Development

verfasst von: Kelly A. Smith, Allen H. Lin, Alexander H. Stevens, S. Michael Yu, Jeffrey A. Weiss, Lucas H. Timmins

Erschienen in: Journal of Cardiovascular Translational Research | Ausgabe 2/2023

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Abstract

Remodeling of extracellular matrix proteins underlies the development of cardiovascular disease. Herein, we utilized a novel molecular probe, collagen hybridizing peptide (CHP), to target collagen molecular damage during atherogenesis. The thoracic aorta was dissected from ApoE^−/− mice that had been on a high-fat diet for 0–18 weeks. Using an optimized protocol, tissues were stained with Cy3-CHP and digested to quantify CHP with a microplate assay. Results demonstrated collagen molecular damage, inferred from Cy3-CHP fluorescence, was a function of location and time on the high-fat diet. Tissue from the aortic arch showed a significant increase in collagen molecular damage after 18 weeks, while no change was observed in tissue from the descending aorta. No spatial differences in fluorescence were observed between the superior and inferior arch tissue. Our results provide insight into the early changes in collagen during atherogenesis and present a new opportunity in the subclinical diagnosis of atherosclerosis.

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Titel: Collagen Molecular Damage is a Hallmark of Early Atherosclerosis Development
verfasst von: Kelly A. Smith
Allen H. Lin
Alexander H. Stevens
S. Michael Yu
Jeffrey A. Weiss
Lucas H. Timmins
Publikationsdatum: 12.09.2022
Verlag: Springer US
Erschienen in: Journal of Cardiovascular Translational Research / Ausgabe 2/2023
Print ISSN: 1937-5387
Elektronische ISSN: 1937-5395
DOI: https://doi.org/10.1007/s12265-022-10316-y

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Collagen Molecular Damage is a Hallmark of Early Atherosclerosis Development

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