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Inflammation Research

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01.11.2012 | Original Research Paper

Combined use of etanercept and MTX restores CD4⁺/CD8⁺ ratio and Tregs in spleen and thymus in collagen-induced arthritis

verfasst von: B. Huang, Q. T. Wang, S. S. Song, Y. J. Wu, Y. K. Ma, L. L. Zhang, J. Y. Chen, H. X. Wu, L. Jiang, W. Wei

Erschienen in: Inflammation Research | Ausgabe 11/2012

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Abstract

Objective

To further explore the mechanism of etanercept (ENT, rhTNFR:Fc) and methotrexate (MTX) in the combined treatment of rheumatoid arthritis (RA), we investigated whether thymic and splenic T-cell subsets and their related cytokines imbalance could be restored by ETN/MTX treatment.

Methods

The effect of ETN/MTX on collagen-induced arthritis (CIA) was evaluated by arthritis scores, joint and spleen histopathology, as well as indices of thymus and spleen. T lymphocytes proliferation was determined by [³H]-TdR incorporation. Levels of TNF-α, LT-α, IL-1β, RANKL, IL-10, IL-17, IFN-γ and IL-6 were detected by enzyme linked immunosorbent assay. The subsets of T lymphocytes including CD4⁺, CD8⁺, CD3⁺CD4⁺, CD4⁺CD25⁺, CD4⁺CD62L⁺ and CD4⁺CD25⁺Foxp3⁺ cells were quantified using flow cytometry.

Results

Combined administration of ETN/MTX significantly inhibited the proliferation of T lymphocytes, decreased serum IL-6, TNF-α, IL-1β, RANKL and macrophage supernatant IL-17, LT-α, increased serum IFN-γ and macrophage supernatant IL-10. Moreover, the combined administration could restore CD4⁺/CD8⁺ ratio and Treg cells of CIA thymus and spleen.

Conclusion

Taken together, our findings suggest that ENT/MTX may modify the abnormal T lymphocytes balance from central to peripheral lymphoid organs, which may partially, explained the mechanism of the combined administration.

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Titel: Combined use of etanercept and MTX restores CD4+/CD8+ ratio and Tregs in spleen and thymus in collagen-induced arthritis
verfasst von: B. Huang
Q. T. Wang
S. S. Song
Y. J. Wu
Y. K. Ma
L. L. Zhang
J. Y. Chen
H. X. Wu
L. Jiang
W. Wei
Publikationsdatum: 01.11.2012
Verlag: SP Birkhäuser Verlag Basel
Erschienen in: Inflammation Research / Ausgabe 11/2012
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-012-0520-0

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