nach oben

Erschienen in:

01.09.2015 | Original Article

Construction and characterization of a recombinant reticuloendotheliosis virus expressing enhanced green fluorescent protein

verfasst von: Xiaoyun Deng, Feng Hu, Xiaole Qi, Li Gao, Kai Li, Honglei Gao, Yulong Gao, Yongqiang Wang, Nan Shen, Yuping Hua, Xiaomei Wang

Erschienen in: Archives of Virology | Ausgabe 9/2015

Einloggen, um Zugang zu erhalten

Abstract

Reticuloendotheliosis virus (REV) causes an immunosuppressive and oncogenic disease in chickens and other birds. In this study, based on an infectious clone of REV, named HLJR0901, a recombinant virus containing the enhanced green fluorescence protein (EGFP) gene was constructed by inserting the EGFP expression cassette downstream of the 3′ terminus of the viral env gene. An EGFP-tagged REV that stably expresses EGFP was rescued. This visible recombinant REV could contribute to the further understanding of the molecular mechanism involved in the replication and pathogenicity of REV.

Bohls RL, Linares JA, Gross SL, Ferro PJ, Silvy NJ, Collisson EW (2006) Phylogenetic analyses indicate little variation among reticuloendotheliosis viruses infecting avian species, including the endangered Attwater’s prairie chicken. Virus Res 119:187–194CrossRefPubMed

Buscaglia C (2013) Mixed Infections of Marek’s disease and reticuloendotheliosis viruses in layer flocks in Argentina. Avian Dis 57:569–571CrossRefPubMed

Cheng WH, Huang YP, Wang CH (2006) Serological and virological surveys of reticuloendotheliosis in chickens in Taiwan. J Vet Med Sci 68:1315–1320CrossRefPubMed

Cui Z, Sun S, Zhang Z, Meng S (2009) Simultaneous endemic infections with subgroup J avian leukosis virus and reticuloendotheliosis virus in commercial and local breeds of chickens. Avian Pathol 38:443–448CrossRefPubMed

Deng X, Qi X, Wu G, Gao Y, Qin L, Wang Y, Gao H, Wang X (2012) Construction and characterization of the infectious clone of Reticuloendotheliosis virus carrying a genetic marker. Virus Res 167:146–151CrossRefPubMed

Dong X, Ju S, Zhao P, Li Y, Meng F, Sun P, Cui Z (2014) Synergetic effects of subgroup J avian leukosis virus and reticuloendotheliosis virus co-infection on growth retardation and immunosuppression in SPF chickens. Vet Microbiol 172:425–431CrossRefPubMed

Fang Y, Rowland RRR, Roof M, Lunney JK, Christopher-Hennings J, Nelson EA (2006) A full-length cDNA infectious clone of North American type 1 porcine reproductive and respiratory syndrome virus: Expression of green fluorescent protein in the Nsp2 region. J Virol 80:11447–11455PubMedCentralCrossRefPubMed

Jiang L, Deng X, Gao Y, Li K, Chai H, Fan Z, Ren X, Wang Q, Zhang L, Yun B, Yin C, Chen Y, Qin L, Gao H, Wang Y, Hua Y, Wang X (2014) First isolation of reticuloendotheliosis virus from mallards in China. Arch Virol 159:2051–2057CrossRefPubMed

Jiang T, Lu X, Yuan Y, Zheng L, Shi J, Zhang D (2012) Complete genomic sequence of a Muscovy duck-origin reticuloendotheliosis virus from China. J Virol 86:13140–13141PubMedCentralCrossRefPubMed

10.

Jin J, Sherer NM, Heidecker G, Derse D, Mothes W (2009) Assembly of the murine leukemia virus Is directed towards sites of cell-cell contact. Plos Biol 7:e1000163PubMedCentralCrossRefPubMed

11.

Koo BS, Lee HR, Jeon EO, Jang HS, Han MS, Min KC, Lee SB, Kim JJ, Mo IP (2013) An outbreak of lymphomas in a layer chicken flock previously infected with fowlpox virus containing integrated reticuloendotheliosis virus. Avian Dis 57:812–817CrossRefPubMed

12.

Li J, Yang C, Li Q, Li H, Xia Y, Liu D, Yu K, Yang H (2013) Complete genome sequence of reticuloendotheliosis virus strain MD-2, isolated from a contaminated turkey herpesvirus vaccine. Genome Announc 1:e00785PubMedCentralPubMed

13.

Li K, Gao H, Gao L, Qi X, Gao Y, Qin L, Wang Y, Wang X (2012) Development of an indirect ELISA for serological detection of reticuloendotheliosis virus using the gp90 protein expressed in Pichia pastoris. J Viroll Methods 180:43–48CrossRef

14.

Li Y, Shen L, Sun Y, Yuan J, Huang J, Li C, Li S, Luo Y, Qiu H-J (2013) Simplified serum neutralization test based on enhanced green fluorescent protein-tagged classical swine fever virus. J Clin Microbiol 51:2710–2712PubMedCentralCrossRefPubMed

15.

Liu Q, Zhao J, Su J, Pu J, Zhang G, Liu J (2009) Full genome sequences of two reticuloendotheliosis viruses contaminating commercial vaccines. Avian Dis 53:341–346CrossRefPubMed

16.

McDonald D, Vodicka MA, Lucero G, Svitkina TM, Borisy GG, Emerman M, Hope TJ (2002) Visualization of the intracellular behavior of HIV in living cells. J Cell Biol 159:441–452PubMedCentralCrossRefPubMed

17.

Moradpour D, Evans MJ, Gosert R, Yuan ZH, Blum HE, Goff SP, Lindenbach BD, Rice CM (2004) Insertion of green fluorescent protein into nonstructural protein 5A allows direct visualization of functional hepatitis C virus replication complexes. J Virol 78:7400–7409PubMedCentralCrossRefPubMed

18.

Mzula A, Masola SN, Kasanga CJ, Wambura PN (2014) Existence of variant strains Fowlpox virus integrated with Reticuloendotheliosis virus in its genome in field isolates in Tanzania. Trop Anim Health Pro 46:711–716CrossRef

19.

Qi X, Gao W, Gao H, Deng X, Bu Z, Wang X, Fu C, Wang X (2007) An improved method for infectious bursal disease virus rescue using RNA polymerase II system. J Virol Methods 142:81–88CrossRefPubMed

20.

Wang Q, Li X, Ji X, Wang J, Shen N, Gao Y, Qi X, Wang Y, Gao H, Zhang S, Wang (2015) A recombinant avian leukosis virus subgroup j for directly monitoring viral infection and the selection of neutralizing antibodies. Plos One 9:e115422CrossRef

21.

Zavala G, Cheng S, Barbosa T, Haefele H (2006) Enzootic reticuloendotheliosis in the endangered Attwater’s and greater prairie chickens. Avian Dis 50:520–525CrossRefPubMed

Titel: Construction and characterization of a recombinant reticuloendotheliosis virus expressing enhanced green fluorescent protein
verfasst von: Xiaoyun Deng
Feng Hu
Xiaole Qi
Li Gao
Kai Li
Honglei Gao
Yulong Gao
Yongqiang Wang
Nan Shen
Yuping Hua
Xiaomei Wang
Publikationsdatum: 01.09.2015
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 9/2015
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-015-2502-3

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Construction and characterization of a recombinant reticuloendotheliosis virus expressing enhanced green fluorescent protein

Abstract

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Erhebliches Risiko für Kehlkopfkrebs bei mäßiger Dysplasie

Nach Herzinfarkt mit Typ-1-Diabetes schlechtere Karten als mit Typ 2?

15% bedauern gewählte Blasenkrebs-Therapie

Costims – das nächste heiße Ding in der Krebstherapie?

Update Innere Medizin

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 9/2015

Complete genome sequence of a novel dsRNA mycovirus isolated from the phytopathogenic fungus Fusarium oxysporum f. sp. dianthi

Molecular characterization of a novel mycovirus from Rhizoctonia fumigata AG-Ba isolate C-314 Baishi

Glycoprotein from street rabies virus BD06 induces early and robust immune responses when expressed from a non-replicative adenovirus recombinant

Diagnosis of porcine circovirus type 2 infection with a combination of immunomagnetic beads, single-domain antibody, and fluorescent quantum dot probes

Decline in Helicoverpa armigera nucleopolyhedrovirus occlusion body yields with increasing infection cell density in vitro is strongly correlated with viral DNA levels

Repurposing of rutin for the inhibition of norovirus replication

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Erhebliches Risiko für Kehlkopfkrebs bei mäßiger Dysplasie

Nach Herzinfarkt mit Typ-1-Diabetes schlechtere Karten als mit Typ 2?

15% bedauern gewählte Blasenkrebs-Therapie

Costims – das nächste heiße Ding in der Krebstherapie?

Update Innere Medizin