Fig. 4
A Expression of cardiac marker cardiac troponin (green-cTnT) in iPSCs-derived cardiomyocytes with nucleus (blue) was observed. Flowcytometry analysis showed more than 80% expression of cardiac troponin in iPSCs-derived Cardiomyocytes. B Representative calcium-flux signal traces (average fluorescence intensities) for cardiotoxic compound-Doxorubicin. Traces shown are typical phenotypic responses including unaffected regular Ca2+ flux patterns, and affected doxorubicin treated iPSC-derived cardiomyocytes (Control, Ovarian cancer and Breast cancer) patterns, Scale bar: 100 μm. C Representative calcium-flux signal traces (average fluorescence intensities) for chemotherapeutic cardiotoxic drugs. Traces shown are typical phenotypic responses including untreated regular Ca2+ flux patterns, and treated doxorubicin patterns