Erschienen in:
01.12.2003 | Article
Cytomegalovirus Infection Induces Production of Human Interleukin-10 in Macrophages
verfasst von:
I. Nordøy, H. Rollag, E. Lien, H. Sindre, M. Degré, P. Aukrust, S. S. Frøland, F. Müller
Erschienen in:
European Journal of Clinical Microbiology & Infectious Diseases
|
Ausgabe 12/2003
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Abstract
Earlier findings have suggested that the balance between interleukin-10 and tumor necrosis factor α levels in serum may influence the outcome of cytomegalovirus infection in renal transplant recipients. Therefore, the aim of the present study was to investigate whether human cytomegalovirus induces interleukin-10 production in macrophages. Experiments using human cytomegalovirus (strain 2006), ultraviolet-inactivated cytomegalovirus, and mock-infected differentiated THP-1 cells with or without ganciclovir or monoclonal anti-tumor necrosis factor α antibodies were performed. Cytomegalovirus-infected cells produced significantly higher levels of human interleukin-10 mRNA and interleukin-10 than ultraviolet-inactivated cytomegalovirus or mock-infected cells. The addition of ganciclovir had little effect on interleukin-10 production. Anti-tumor necrosis factor α antibodies appeared to reduce the interleukin-10 levels. In conclusion, human cytomegalovirus infection of macrophages induces production of human interleukin-10. This requires viral entry, but not full viral replication.