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Archives of Virology

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18.01.2016 | Original Article

Detection and molecular characterization of astroviruses in turkeys

verfasst von: Azad Singh, Sunil K. Mor, Naresh Jindal, Devi Patnayak, Nader M. Sobhy, Nhungoc Ti Luong, Sagar M. Goyal

Erschienen in: Archives of Virology | Ausgabe 4/2016

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Abstract

This study was conducted to determine the prevalence and molecular characteristics of turkey astrovirus 1 (TAstV-1) and avian nephritis virus (ANV) in turkeys with light turkey syndrome (LTS), which is characterized by lower body weight in market-age turkeys than their standard breed character. We collected pools of fecal samples from four LTS and two non-LTS turkey flocks in Minnesota at 2, 3, 5 and 8 weeks of age. Of the 80 LTS pools tested, 16 (20.0 %) and 11 (13.8 %) were positive for TAstV-1 and ANV, respectively. For non-LTS flocks, these numbers were 8 (20.0 %) and 5 (12.5 %), respectively. The maximum number of birds was positive at five weeks of age. We also tested 130 fecal samples of poult enteritis syndrome (PES) cases submitted to the Minnesota Veterinary Diagnostic Laboratory and found 19 and 11 positive for TAstV-1 and ANV, respectively. RdRp gene sequences were determined for a total of 29 TAstV-1 and 22 ANV samples. Phylogenetic analysis of the RdRp gene revealed 92-100 % and 88-100 % nucleotide sequence identity among TAstV-1 and ANV sequences, respectively. A large number of nucleotide and amino acid substitutions were observed in LTS and PES flocks than in non-LTS flocks. One of the PES sequences grouped with ANV-like sequences detected in chickens, indicating that regular screening of birds should be continued. Further, complete genome analysis should be conducted to determine whether this virus is a novel divergent strain or a recombinant of chicken and turkey ANV-like viruses. The detection of TAstV-1 and ANV in a considerable number of non-LTS cases emphasizes the need for further studies on the transmission pattern and pathogenesis of these viruses to determine their role as pathogens of turkeys.

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Titel: Detection and molecular characterization of astroviruses in turkeys
verfasst von: Azad Singh
Sunil K. Mor
Naresh Jindal
Devi Patnayak
Nader M. Sobhy
Nhungoc Ti Luong
Sagar M. Goyal
Publikationsdatum: 18.01.2016
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 4/2016
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-016-2753-7

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