Skip to main content
Hauptrubriken
CME Facharzt-Training Webinare Zeitschriften Bücher e.Medpedia Podcast
Service
Jobbörse Info & Hilfe
Fachgebiete
Anästhesiologie Allgemeinmedizin Arbeitsmedizin Augenheilkunde Chirurgie Dermatologie Gynäkologie und Geburtshilfe HNO Innere Medizin Kardiologie Neurologie Onkologie und Hämatologie Orthopädie und Unfallchirurgie Pädiatrie Pathologie Psychiatrie Radiologie Rechtsmedizin Urologie Zahnmedizin
Themen
Klimawandel und Gesundheit Neues aus dem Markt COVID-19 Praxis und Beruf Seltene Erkrankungen GOÄ & EBM Panorama
Sammlungen
Kasuistiken Algorithmen & Infografiken Blickdiagnosen Arthropedia FlapFinder (für Dermatochirurgen) Videos Kongressberichterstattung Medizin für Apothekerinnen und Apotheker Für Ärztinnen und Ärzte in Weiterbildung Für Medizinstudierende
Erweiterte Suche
Anmelden
nach oben
Medical Microbiology and Immunology
Erschienen in: Medical Microbiology and Immunology 3/2014

01.06.2014 | Original Investigation

Detection of Mycobacterium avium subspecies paratuberculosis in patients with Crohn’s disease is unrelated to the presence of single nucleotide polymorphisms rs2241880 (ATG16L1) and rs10045431 (IL12B)

verfasst von: James P. Dalton, Alan Desmond, Fergus Shanahan, Colin Hill

Erschienen in: Medical Microbiology and Immunology | Ausgabe 3/2014

Einloggen, um Zugang zu erhalten

Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) has been controversially linked with Crohn’s disease (CD). Detection of MAP in CD has been highly variable, and one explanation might be the genetic heterogeneity of this syndrome. Many of the single nucleotide polymorphisms (SNPs) linked with CD are contained within genes that are associated with bacterial handling in general, and some are specifically implicated in susceptibility to mycobacterial disease. We tested a cohort of IBD patients (n = 149) to determine whether the presence of MAP was associated with a selection of these SNPs. Blood samples from CD patients (n = 84), ulcerative colitis (UC, n = 65) patients and healthy controls (n = 55) were examined for the presence of MAP and SNPs in ATG16L1, IL12B, NOD2/CARD15, NKx2-3, IL23R and IRGM. Statistical analysis was then used to determine whether there was any association between the presence of MAP and these SNPs. MAP, rs2241880 (ATG16L1) and rs10045431 (IL12B) were found to be significantly associated with CD. The presence of MAP was not related to the status of the SNPs in ATG16L1 or IL12B. We have found no evidence for the contribution of these SNPs to the presence of MAP in CD patients.
Literatur
1.
2.
3.
Lupp C, Robertson ML, Wickham ME, Sekirov I, Champion OL, Gaynor EC, Finlay BB (2007) Host-mediated inflammation disrupts the intestinal microbiota and promotes the overgrowth of Enterobacteriaceae. Cell Host Microbe 2(2):119–129. doi:10.​1016/​j.​chom.​2007.​06.​010 PubMedCrossRef
4.
5.
Jostins L, Ripke S, Weersma RK, Duerr RH, McGovern DP, Hui KY, Lee JC, Schumm LP, Sharma Y, Anderson CA, Essers J, Mitrovic M, Ning K, Cleynen I, Theatre E, Spain SL, Raychaudhuri S, Goyette P, Wei Z, Abraham C, Achkar JP, Ahmad T, Amininejad L, Ananthakrishnan AN, Andersen V, Andrews JM, Baidoo L, Balschun T, Bampton PA, Bitton A, Boucher G, Brand S, Buning C, Cohain A, Cichon S, D’Amato M, De Jong D, Devaney KL, Dubinsky M, Edwards C, Ellinghaus D, Ferguson LR, Franchimont D, Fransen K, Gearry R, Georges M, Gieger C, Glas J, Haritunians T, Hart A, Hawkey C, Hedl M, Hu X, Karlsen TH, Kupcinskas L, Kugathasan S, Latiano A, Laukens D, Lawrance IC, Lees CW, Louis E, Mahy G, Mansfield J, Morgan AR, Mowat C, Newman W, Palmieri O, Ponsioen CY, Potocnik U, Prescott NJ, Regueiro M, Rotter JI, Russell RK, Sanderson JD, Sans M, Satsangi J, Schreiber S, Simms LA, Sventoraityte J, Targan SR, Taylor KD, Tremelling M, Verspaget HW, De Vos M, Wijmenga C, Wilson DC, Winkelmann J, Xavier RJ, Zeissig S, Zhang B, Zhang CK, Zhao H, Silverberg MS, Annese V, Hakonarson H, Brant SR, Radford-Smith G, Mathew CG, Rioux JD, Schadt EE, Daly MJ, Franke A, Parkes M, Vermeire S, Barrett JC, Cho JH (2012) Host-microbe interactions have shaped the genetic architecture of inflammatory bowel disease. Nature 491(7422):119–124. doi:10.​1038/​nature11582 PubMedCentralPubMedCrossRef
6.
7.
Bentley RW, Keenan JI, Gearry RB, Kennedy MA, Barclay ML, Roberts RL (2008) Incidence of Mycobacterium avium subspecies paratuberculosis in a population-based cohort of patients with Crohn’s disease and control subjects. Am J Gastroenterol 103(5):1168–1172PubMedCrossRef
8.
Chamberlin W, Naser SA (2008) Blood cultures of 19 Crohn’s disease patients. Am J Gastroenterol 103(3):802–803PubMed
9.
Di Sabatino A, Paccagnini D, Vidali F, Rosu V, Biancheri P, Cossu A, Zanetti S, Corazza GR, Sechi LA (2011) Detection of Mycobacterium avium subsp. paratuberculosis (MAP)-specific IS900 DNA and antibodies against MAP peptides and lysate in the blood of Crohn’s disease patients. Inflamm Bowel Dis 17(5):1254–1255. doi:10.​1002/​ibd.​21461
10.
Kirkwood CD, Wagner J, Boniface K, Vaughan J, Michalski WP, Catto-Smith AG, Cameron DJ, Bishop RF (2009) Mycobacterium avium subspecies paratuberculosis in children with early-onset Crohn’s disease. Inflamm Bowel Dis 15(11):1643–1655PubMedCrossRef
11.
Mendoza JL, San-Pedro A, Culebras E, Cies R, Taxonera C, Lana R, Urcelay E, de la Torre F, Picazo JJ, Diaz-Rubio M (2010) High prevalence of viable Mycobacterium avium subspecies paratuberculosis in Crohn’s disease. World J Gastroenterol 16(36):4558–4563PubMedCentralPubMedCrossRef
12.
Naser SA, Ghobrial G, Romero C, Valentine JF (2004) Culture of Mycobacterium avium subspecies paratuberculosis from the blood of patients with Crohn’s disease. Lancet 364(9439):1039–1044PubMedCrossRef
13.
Naser SA, Schwartz D, Shafran I (2000) Isolation of Mycobacterium avium subsp. paratuberculosis from breast milk of Crohn’s disease patients. Am J Gastroenterol 95(4):1094–1095PubMedCrossRef
14.
Hampe J, Franke A, Rosenstiel P, Till A, Teuber M, Huse K, Albrecht M, Mayr G, De La Vega FM, Briggs J, Gunther S, Prescott NJ, Onnie CM, Hasler R, Sipos B, Folsch UR, Lengauer T, Platzer M, Mathew CG, Krawczak M, Schreiber S (2007) A genome-wide association scan of nonsynonymous SNPs identifies a susceptibility variant for Crohn disease in ATG16L1. Nat Genet 39(2):207–211PubMedCrossRef
15.
Barrett JC, Hansoul S, Nicolae DL, Cho JH, Duerr RH, Rioux JD, Brant SR, Silverberg MS, Taylor KD, Barmada MM, Bitton A, Dassopoulos T, Datta LW, Green T, Griffiths AM, Kistner EO, Murtha MT, Regueiro MD, Rotter JI, Schumm LP, Steinhart AH, Targan SR, Xavier RJ, Libioulle C, Sandor C, Lathrop M, Belaiche J, Dewit O, Gut I, Heath S, Laukens D, Mni M, Rutgeerts P, Van Gossum A, Zelenika D, Franchimont D, Hugot JP, de Vos M, Vermeire S, Louis E, Cardon LR, Anderson CA, Drummond H, Nimmo E, Ahmad T, Prescott NJ, Onnie CM, Fisher SA, Marchini J, Ghori J, Bumpstead S, Gwilliam R, Tremelling M, Deloukas P, Mansfield J, Jewell D, Satsangi J, Mathew CG, Parkes M, Georges M, Daly MJ (2008) Genome-wide association defines more than 30 distinct susceptibility loci for Crohn’s disease. Nat Genet 40(8):955–962PubMedCentralPubMedCrossRef
16.
T.W.T.C.C.C. (2007) Genome-wide association study of 14,000 cases of seven common diseases and 3,000 shared controls. Nature 447(7145):661–678CrossRef
17.
Abreu MT, Taylor KD, Lin YC, Hang T, Gaiennie J, Landers CJ, Vasiliauskas EA, Kam LY, Rojany M, Papadakis KA, Rotter JI, Targan SR, Yang H (2002) Mutations in NOD2 are associated with fibrostenosing disease in patients with Crohn’s disease. Gastroenterology 123(3):679–688PubMedCrossRef
18.
Angelberger S, Reinisch W, Dejaco C, Miehsler W, Waldhoer T, Wehkamp J, Lichtenberger C, Schaeffeler E, Vogelsang H, Schwab M, Teml A (2008) NOD2/CARD15 gene variants are linked to failure of antibiotic treatment in perianal fistulating Crohn’s disease 103(5):1197–1202
19.
Hlavaty T, Pierik M, Henckaerts L, Ferrante M, Joossens S, Van Schuerbeek N, Noman M, Rutgeerts P, Vermeire S (2005) Polymorphisms in apoptosis genes predict response to infliximab therapy in luminal and fistulizing Crohn’s disease. Aliment Pharmacol Ther 22(7):613–626. doi:10.​1111/​j.​1365-2036.​2005.​02635.​x
20.
Herrlinger KR, Cummings JR, Barnardo MC, Schwab M, Ahmad T, Jewell DP (2005) The pharmacogenetics of methotrexate in inflammatory bowel disease. Pharmacogenet Genomics 15(10):705–711PubMedCrossRef
21.
22.
Ryan P, Aarons S, Bennett MW, Lee G, O’Sullivan GC, O’Connell J, Shanahan F (2002) Mycobacterium paratuberculosis detected by nested PCR in intestinal granulomas isolated by LCM in cases of Crohn’s disease. Methods Mol Biol 193:205–211. doi:10.​1385/​1-59259-283-x:​205 PubMed
23.
Sibartie S, Scully P, Keohane J, O’Neill S, O’Mahony J, O’Hanlon D, Kirwan WO, O’Mahony L, Shanahan F (2010) Mycobacterium avium subsp. paratuberculosis (MAP) as a modifying factor in Crohn’s disease. Inflamm Bowel Dis 16(2):296–304. doi:10.​1002/​ibd.​21052 PubMedCrossRef
24.
25.
Singh UP, Singh S, Singh R, Karls RK, Quinn FD, Potter ME, Lillard JW Jr (2007) Influence of Mycobacterium avium subsp. paratuberculosis on colitis development and specific immune responses during disease. Infect Immun 75(8):3722–3728. doi:10.​1128/​iai.​01770-06 PubMedCentralPubMedCrossRef
26.
Shankar H, Singh SV, Singh PK, Singh AV, Sohal JS, Greenstein RJ (2010) Presence, characterization, and genotype profiles of Mycobacterium avium subspecies paratuberculosis from unpasteurized individual and pooled milk, commercial pasteurized milk, and milk products in India by culture, PCR, and PCR-REA methods. Int J Infect Dis 14(2):e121–e126PubMedCrossRef
27.
Ellingson JL, Anderson JL, Koziczkowski JJ, Radcliff RP, Sloan SJ, Allen SE, Sullivan NM (2005) Detection of viable Mycobacterium avium subsp. paratuberculosis in retail pasteurized whole milk by two culture methods and PCR. J Food Prot 68(5):966–972PubMed
28.
Pickup RW, Rhodes G, Arnott S, Sidi-Boumedine K, Bull TJ, Weightman A, Hurley M, Hermon-Taylor J (2005) Mycobacterium avium subsp. paratuberculosis in the catchment area and water of the River Taff in South Wales, United Kingdom, and its potential relationship to clustering of Crohn’s disease cases in the city of Cardiff. Appl Environ Microbiol 71(4):2130–2139PubMedCentralPubMedCrossRef
29.
Pierce ES (2009) Possible transmission of Mycobacterium avium subspecies paratuberculosis through potable water: lessons from an urban cluster of Crohn’s disease. Gut Pathog 1(1):17PubMedCentralPubMedCrossRef
30.
Beumer A, King D, Donohue M, Mistry J, Covert T, Pfaller S (2010) Detection of Mycobacterium avium subsp. paratuberculosis in drinking water and biofilms by quantitative PCR. Appl Environ Microbiol 76(21):7367–7370PubMedCentralPubMedCrossRef
31.
Pickup RW, Rhodes G, Bull TJ, Arnott S, Sidi-Boumedine K, Hurley M, Hermon-Taylor J (2006) Mycobacterium avium subsp. paratuberculosis in lake catchments, in river water abstracted for domestic use, and in effluent from domestic sewage treatment works: diverse opportunities for environmental cycling and human exposure. Appl Environ Microbiol 72(6):4067–4077PubMedCentralPubMedCrossRef
32.
Bull TJ, McMinn EJ, Sidi-Boumedine K, Skull A, Durkin D, Neild P, Rhodes G, Pickup R, Hermon-Taylor J (2003) Detection and verification of Mycobacterium avium subsp. paratuberculosis in fresh ileocolonic mucosal biopsy specimens from individuals with and without Crohn’s disease. J Clin Microbiol 41(7):2915–2923PubMedCentralPubMedCrossRef
33.
Stabel JR, Bannantine JP (2005) Development of a nested PCR method targeting a unique multicopy element, ISMap02, for detection of Mycobacterium avium subsp. paratuberculosis in fecal samples. J Clin Microbiol 43(9):4744–4750PubMedCentralPubMedCrossRef
34.
35.
Amre DK, Mack DR, Morgan K, Israel D, Deslandres C, Seidman EG, Lambrette P, Costea I, Krupoves A, Fegury H, Dong J, Grimard G, Levy E (2010) Susceptibility loci reported in genome-wide association studies are associated with Crohn’s disease in Canadian children. Aliment Pharmacol Ther 31(11):1186–1191PubMedCrossRef
36.
Moon CM, Shin DJ, Son NH, Shin ES, Hong SP, Kim TI, Kim WH, Cheon JH (2013) Genetic variants in the IL12B gene are associated with inflammatory bowel diseases in the Korean population. J Gastroenterol Hepatol. doi:10.​1111/​jgh.​12214
37.
Peter I, Mitchell AA, Ozelius L, Erazo M, Hu J, Doheny D, Abreu MT, Present DH, Ullman T, Benkov K, Korelitz BI, Mayer L, Desnick RJ (2011) Evaluation of 22 genetic variants with Crohn’s disease risk in the Ashkenazi Jewish population: a case-control study. BMC Med Genet 12:63. doi:10.​1186/​1471-2350-12-63 PubMedCentralPubMedCrossRef
38.
39.
Danoy P, Pryce K, Hadler J, Bradbury LA, Farrar C, Pointon J, Ward M, Weisman M, Reveille JD, Wordsworth BP, Stone MA, Maksymowych WP, Rahman P, Gladman D, Inman RD, Brown MA (2010) Association of variants at 1q32 and STAT3 with ankylosing spondylitis suggests genetic overlap with Crohn’s disease. PLoS Genet 6(12):e1001195PubMedCentralPubMedCrossRef
40.
Tso HW, Lau YL, Tam CM, Wong HS, Chiang AK (2004) Associations between IL12B polymorphisms and tuberculosis in the Hong Kong Chinese population. J Infect Dis 190(5):913–919PubMedCrossRef
41.
Wang J, Tang S, Shen H (2010) Association of genetic polymorphisms in the IL12-IFNG pathway with susceptibility to and prognosis of pulmonary tuberculosis in a Chinese population. Eur J Clin Microbiol Infect Dis 29(10):1291–1295PubMedCrossRef
42.
Morahan G, Kaur G, Singh M, Rapthap CC, Kumar N, Katoch K, Mehra NK, Huang D (2007) Association of variants in the IL12B gene with leprosy and tuberculosis. Tissue Antigens 69(Suppl 1):234–236PubMedCrossRef
43.
44.
Trinchieri G, Pflanz S, Kastelein RA (2003) The IL-12 family of heterodimeric cytokines: new players in the regulation of T cell responses. Immunity 19(5):641–644PubMedCrossRef
45.
Liu H, Irwanto A, Tian H, Fu X, Yu Y, Yu G, Low H, Chu T, Li Y, Shi B, Chen M, Sun Y, Yuan C, Lu N, You J, Bao F, Li J, Liu J, Liu D, Yu X, Zhang L, Yang Q, Wang N, Niu G, Ma S, Zhou Y, Wang C, Chen S, Zhang X, Zhang F (2012) Identification of IL18RAP/IL18R1 and IL12B as leprosy risk genes demonstrates shared pathogenesis between inflammation and infectious diseases. Am J Hum Genet 91(5):935–941. doi:10.​1016/​j.​ajhg.​2012.​09.​010 PubMedCentralPubMedCrossRef
46.
Cheng JF, Ning YJ, Zhang W, Lu ZH, Lin L (2010) T300A polymorphism of ATG16L1 and susceptibility to inflammatory bowel diseases: a meta-analysis. World J Gastroenterol 16(10):1258–1266PubMedCentralPubMedCrossRef
47.
Cotterill L, Payne D, Levinson S, McLaughlin J, Wesley E, Feeney M, Durbin H, Lal S, Makin A, Campbell S, Roberts SA, O’Neill C, Edwards C, Newman WG (2010) Replication and meta-analysis of 13,000 cases defines the risk for interleukin-23 receptor and autophagy-related 16-like 1 variants in Crohn’s disease. Can J Gastroenterol 24(5):297–302PubMedCentralPubMed
48.
Buning C, Durmus T, Molnar T, de Jong DJ, Drenth JP, Fiedler T, Gentz E, Todorov T, Haas V, Buhner S, Sturm A, Baumgart DC, Nagy F, Lonovics J, Landt O, Kage A, Buning H, Nickel R, Buttner J, Lochs H, Schmidt HH, Witt H (2007) A study in three European IBD cohorts confirms that the ATG16L1 c.898A > G (p.Thr300Ala) variant is a susceptibility factor for Crohn’s disease. J Crohns Colitis 1(2):70–76PubMedCrossRef
49.
Sventoraityte J, Zvirbliene A, Franke A, Kwiatkowski R, Kiudelis G, Kupcinskas L, Schreiber S (2010) NOD2, IL23R and ATG16L1 polymorphisms in Lithuanian patients with inflammatory bowel disease. World J Gastroenterol 16(3):359–364PubMedCentralPubMedCrossRef
50.
51.
52.
53.
Abubakar I, Myhill D, Aliyu SH, Hunter PR (2008) Detection of Mycobacterium avium subspecies paratuberculosis from patients with Crohn’s disease using nucleic acid-based techniques: a systematic review and meta-analysis. Inflamm Bowel Dis 14(3):401–410PubMedCrossRef
54.
Ottenhoff TH, Verreck FA, Hoeve MA, van de Vosse E (2005) Control of human host immunity to mycobacteria. Tuberculosis 85(1–2):53–64PubMedCrossRef
55.
Lesage S, Zouali H, Cezard JP, Colombel JF, Belaiche J, Almer S, Tysk C, O’Morain C, Gassull M, Binder V, Finkel Y, Modigliani R, Gower-Rousseau C, Macry J, Merlin F, Chamaillard M, Jannot AS, Thomas G, Hugot JP (2002) CARD15/NOD2 mutational analysis and genotype-phenotype correlation in 612 patients with inflammatory bowel disease. Am J Hum Genet 70(4):845–857PubMedCentralPubMedCrossRef
56.
Parkes M, Barrett JC, Prescott NJ, Tremelling M, Anderson CA, Fisher SA, Roberts RG, Nimmo ER, Cummings FR, Soars D, Drummond H, Lees CW, Khawaja SA, Bagnall R, Burke DA, Todhunter CE, Ahmad T, Onnie CM, McArdle W, Strachan D, Bethel G, Bryan C, Lewis CM, Deloukas P, Forbes A, Sanderson J, Jewell DP, Satsangi J, Mansfield JC, Cardon L, Mathew CG (2007) Sequence variants in the autophagy gene IRGM and multiple other replicating loci contribute to Crohn’s disease susceptibility. Nat Genet 39(7):830–832PubMedCentralPubMedCrossRef
57.
Duerr RH, Taylor KD, Brant SR, Rioux JD, Silverberg MS, Daly MJ, Steinhart AH, Abraham C, Regueiro M, Griffiths A, Dassopoulos T, Bitton A, Yang H, Targan S, Datta LW, Kistner EO, Schumm LP, Lee AT, Gregersen PK, Barmada MM, Rotter JI, Nicolae DL, Cho JH (2006) A genome-wide association study identifies IL23R as an inflammatory bowel disease gene. Science 314(5804):1461–1463PubMedCrossRef
58.
Ferreiros-Vidal I, Garcia-Meijide J, Carreira P, Barros F, Carracedo A, Gomez-Reino JJ, Gonzalez A (2003) The three most common CARD15 mutations associated with Crohn’s disease and the chromosome 16 susceptibility locus for systemic lupus erythematosus. Rheumatology 42(4):570–574PubMedCrossRef
Metadaten
Titel
Detection of Mycobacterium avium subspecies paratuberculosis in patients with Crohn’s disease is unrelated to the presence of single nucleotide polymorphisms rs2241880 (ATG16L1) and rs10045431 (IL12B)
verfasst von
James P. Dalton
Alan Desmond
Fergus Shanahan
Colin Hill
Publikationsdatum
01.06.2014
Verlag
Springer Berlin Heidelberg
Erschienen in
Medical Microbiology and Immunology / Ausgabe 3/2014
Print ISSN: 0300-8584
Elektronische ISSN: 1432-1831
DOI
https://doi.org/10.1007/s00430-014-0332-7

Weitere Artikel der Ausgabe 3/2014

Medical Microbiology and Immunology 3/2014 Zur Ausgabe

Original Investigation

Oral administration of Shiga toxin-producing Escherichia coli induces intestinal and systemic specific immune response in mice

Original Investigation

Interleukin-32 isoforms: expression, interaction with interferon-regulated genes and clinical significance in chronically HIV-1-infected patients

Original Investigation

Cholecystokinin protects rats against sepsis induced by Staphylococcus aureus

Original Investigation

Distinctive cytokine, chemokine, and antibody responses in Echinococcus multilocularis-infected patients with cured, stable, or progressive disease

Original Investigation

Tumor prevention in HPV8 transgenic mice by HPV8-E6 DNA vaccination

Original Investigation

Interferon lambda 1 expression in cervical cells differs between low-risk and high-risk human papillomavirus-positive women

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

25.04.2024 | Hypotonie | Nachrichten

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

25.04.2024 | Hypertonie | Nachrichten

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

25.04.2024 | Nierenkarzinom | Nachrichten

Adjuvante Immuntherapie verlängert Leben bei RCC

25.04.2024 | NSCLC | Nachrichten

Alectinib verbessert krankheitsfreies Überleben bei ALK-positivem NSCLC
weitere anzeigen

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen
Bildnachweise