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Journal of Assisted Reproduction and Genetics

Erschienen in:

01.10.2013 | Fertility Preservation

Does prepubertal testicular tissue vitrification influence spermatogonial stem cells (SSCs) viability?

verfasst von: Mohammadreza Gholami, Masoud Hemadi, Ghasem Saki, Abolfazl Zendedel, Ali Khodadadi, Javad Mohammadi-asl

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 10/2013

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Abstract

Purpose

Testicular cryopreservation prior to chemotherapy or radiotherapy in children with cancer is one of the ways to preserve fertility. However, cryopreservation may cause damage to the testicular parenchyma cells. The objective of this study was to investigate effects of vitrification on the intracellular LDH leakage, cell cycle/apoptotic responses and apoptosis-related gene expression patterns in the spermatogonial stem cells (SSCs) obtained from the vitrified testis.

Methods

The testes of the mice pups (6-day-old, BALB/c) both vitrified and fresh groups were digested with enzymes (collagenase, DNaseΙ, trypsin-EDTA) to disperse the cells. The SSCs, type A, were isolated from the rest of testicular cells by MACS. The amount of damage to the SSCs immediately was evaluated by Cytotoxicity assay, Flow cytometry assay and Real-time PCR.

Results

The intracellular LDH leakage in the SSCs,harvested from the vitrified testes, was less reported compared with the fresh ones. Moreover, the percentage of apoptotic and necrotic SSCs obtained from the vitrified testes was lower than that of yielded from the fresh samples. Also, the apoptosis-related genes of the SSCs,collected from the vitrified testes, changed their expression profile as increasing P53 and BCL-2 expression levels and decreasing Bax and Fas expression levels.

Conclusions

The study indicates that vitrification of prepubertal testicular tissue does not increase the expression profile of apoptosis-related genes such as Bax and Fas in the testicular SSCs consistent with diminished cell apoptotic/necrotic responses and no increasing intracellular LDH leakage.

Abrishami M, Anzar M, Yang Y, Honaramooz A. Cryopreservation of immature porcine testis tissue to maintain its developmental potential after xenografting into recipient mice. Theriogenology. 2009;73:86–96.PubMedCrossRef

Aggarwal A, Misro MM, Maheshwari A, Sehgal N, Nandan D. Adverse effects associated with persistent stimulation of Leydig cells with hCG in vitro. Mol Reprod Dev. 2009;76(11):1076–83.PubMedCrossRef

Amorim CA, Curaba M, Van Langendonckt A, Dolmans MM, Donnez J. Vitrification as an alternative means of cryopreserving ovarian tissue. Reprod BioMed Online. 2011;23:160–86.PubMedCrossRef

Arends MJ, Morris RG, Wyllie AH. Apoptosis. The role of the endonuclease. Am J Pathol. 1990;136:593–608.PubMed

Baert Y, Goossens E, van Saen D, Ning L, in’t Veld P, Tournaye H. Orthotopic grafting of cryopreserved prepubertal testicular tissue: in search of a simple yet effective cryopreservation protocol. Fertil Steril. 2012;97:1152–7.PubMedCrossRef

Bagchi A, Woods EJ, Critser JK. Cryopreservation and vitrification: recent advances in fertility preservation technologies. Expert Rev Med Devices. 2008;5:359–70.PubMedCrossRef

Bank HL, Brockbank KG. Basic principles of cryobiology. J Card Surg. 1987;2:137–43.PubMed

Brougham MF, Kelnar CJ, Sharpe RM, Wallace WH. Male fertility following childhood cancer: current concepts and future therapies. Asian J Androl. 2003;5:325–37.PubMed

Brugnon F, Van Assche E, Verheyen G. Study of two markers of apoptosis and meiotic segregation in ejaculated sperm of chromosomal translocation carrier patients. Hum Reprod. 2006;21:685–93.PubMedCrossRef

10.

Chiarini-Garcia H, Raymer AM, Russell LD. Non-random distribution of spermatogonia in rats: evidence of niches in the seminiferous tubules. Reproduction. 2003;126:669–80.PubMedCrossRef

11.

Curaba M, Verleysen M, Amorim CA, Dolmans MM, Van Langendonckt A, Hovatta O, et al. Cryopreservation of prepubertal mouse testicular tissue by vitrification. Fertil Steril. 2011;95:1229–34.PubMedCrossRef

12.

Dohle GR. Male infertility in cancer patients: Review of the literature. Int J Urol. 2010;17:327–31.PubMedCrossRef

13.

Fuller B, Paynter S. Fundamentals of cryobiology in reproductive medicine. Reprod BioMed Online. 2004;9:680–91.PubMedCrossRef

14.

Gholami M, Saki G, Hemadi M, Khodadadi A, Mohammadi-asl J. Supplementation vitrified-thawed media with melatonin do not protecting immature mouse testicular tissue from vitrified-thawed induced injury. Asian J Anim Vet Adv. 2012;7:940–9.CrossRef

15.

Gouk SS, Loh YF, Kumar SD, Watson PF, Kuleshova LL. Cryopreservation of mouse testicular tissue: prospect for harvesting spermatogonial stem cells for fertility preservation. Fertil Steril. 2011;95:2399–403.PubMedCrossRef

16.

Hemadi M, Saki G. Endocrine function and duration time of estrous cyclicity of the ovariectomized recipiented neonate vitrified ovarian grafts mice after treatment with melatonin. Int J Pharmacol. 2010;6:379–85.CrossRef

17.

Hemadi M, Shokri S, Pourmatroud E, Moramezi F, Khodadai A. Follicular dynamic and immunoreactions of the vitrified ovarian graft after host treatment with variable regimens of melatonin. Am J Reprod Immunol. 2012;67(5):401–12.PubMedCrossRef

18.

Hemadi M, Shokri S, Moramezi F, Nikbakht R, Sobhani A. Potential use of melatonin supplementation to protect vitrified testicular grafts from hypoxic-ischaemic damage. Andrologia. 2013. doi:10.1111/and.12110. [Epub ahead of print].

19.

Hemadi M, Zargar M, Sobhani A, Sobhani A. Assessment of morphological and functional changes in neonate vitrified testis grafts after host treatment with melatonin. Folia Morphol (Warsz). 2011;70(2):95–102.PubMed

20.

Hermann BP, Sukhwani M, Lin CC, Sheng Y, Tomko J, Rodriguez M, et al. Characterization, cryopreservation, and ablation of spermatogonial stem cells in adult rhesus macaques. Stem Cells. 2007;25:2330–8.PubMedCrossRef

21.

Honaramooz A, Behboodi E, Blash S, Megee SO, Dobrinski I. Germ cell transplantation in goats. Mol Reprod Dev. 2003;64:422–8.PubMedCrossRef

22.

Honaramooz A, Behboodi E, Megee SO, Overton SA, Galantino-Homer H, Echelard Y, et al. Fertility and germline transmission of donor haplotype following germ cell transplantation in immunocompetent goats. Biol Reprod. 2003;69:1260–4.PubMedCrossRef

23.

Honaramooz A, Megee SO, Dobrinski I. Germ cell transplantation in pigs. Biol Reprod. 2002;66:21–8.PubMedCrossRef

24.

Honaramooz A, Snedaker A, Boiani M, Scholer H, Dobrinski I, Schlatt S. Sperm from neonatal mammalian testes grafted in mice. Nature. 2002;418:778–81.PubMedCrossRef

25.

Kamischke A, Jurgens H, Hertle L, Berdel WE, Nieschlag E. Cryopreservation of sperm from adolescents and adults with malignancies. J Androl. 2004;25(4):586–92.PubMed

26.

Kasai M, Komi JH, Takakamo A, Tsudera H, Sakurai T, Machida T. A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J Reprod Fertil. 1990;89:91–7.PubMedCrossRef

27.

Kelleher S, Wishart SM, Liu PY, Turner L, Di Pierro I, Conway AJ, et al. Long-term outcomes of elective human sperm cryostorage. Hum Reprod. 2001;16:2632–9.PubMedCrossRef

28.

Kevin MR, Andrew CP, Karen HV. Regulation and function of the p53 tumor suppressor protein. Curr Opin Cell Biol. 2001;13:332–7.CrossRef

29.

Larman MG, Katz-Jaffe MG, McCallie B, Filipovits JA, Gardner DK. Analysis of global gene expression following mouse blastocyst cryopreservation. Hum Reprod. 2011;26:2672–80.PubMedCrossRef

30.

Lee SJ, Schover LR, Partridge AH, Patrizio P, Wallace WH, Hagerty K, et al. American Society of Clinical Oncology recommendations on fertility preservation in cancer patients. J Clin Oncol. 2006;24:2917–31.PubMedCrossRef

31.

Mauduit C, Siah A, Foch M, Chapet O, Clippe S, Gerard JP, et al. Differential expression of growth factors in irradiated mouse testes. Int J Radiat Oncol Biol Phys. 2001;50:203–12.PubMedCrossRef

32.

Martincic DS, VirantKlun I, Zorn B, Vrtovec HM. Germ cell apoptosis in the human testis. Pflugers Arch. 2001;442:159–60.CrossRef

33.

Milazzo JP, Vaudreuil L, Cauliez B, Gruel E, Masse L, Mousset-Simeon N, et al. Comparison of conditions for cryopreservation of testicular tissue from immature mice. Hum Reprod. 2008;23:17–28.PubMedCrossRef

34.

Mitchell RT, Saunders PT, Sharpe RM, Kelnar CJ, Wallace WH. Male fertility and strategies for fertility preservation following childhood cancer treatment. Endocr Dev. 2009;15:101–34.PubMedCrossRef

35.

Nakano K, Balint E, Ashcroft M, Vousden KH. A ribonucleotide reductase gene is a transcriptional target of p53 and p73. Oncogene. 2000;19:4283–9.PubMedCrossRef

36.

Pegg DE. The history and principles of cryopreservation. Semin Reprod Med. 2002;20:5–13.PubMedCrossRef

37.

Pegg DE. Principles of cryopreservation. Methods Mol Biol. 2007;368:39–57.PubMedCrossRef

38.

Pfaffl MW. Relative quantification. In: Dorak T, editors. Real-time PCR. International University Line 2004;2:63–82.

39.

Poels J, Van Langendonckt A, Many MC, Wese FX, Wyns C. Vitrification preserves proliferation capacity in human spermatogonia. Hum Reprod. 2013;28(3):578–89.PubMedCrossRef

40.

Reis LM, Krapco D, Mariotto M, Miller A, Feuer BA, Clegg EJ. SEER cancer statistics review, 1975-2004. National Cancer Institute; 2007.

41.

Schlatt S, Honaramooz A, Boiani M, Scholer HR, Dobrinski I. Progeny from sperm obtained after ectopic grafting of neonatal mouse testes. Biol Reprod. 2003;68:2331–5.PubMedCrossRef

42.

Shinohara T, Avrbock MR, Brinster RL. beta1- and alpha6-integrin are surface markers on mouse spermatogonial stem cells. Proc Natl Acad Sci USA. 1999;96:5504–9.PubMedCrossRef

43.

Shokri S, Hemadi M, Bayat G, Bahmanzadeh M, Jafari-Anarkooli I, Mashkani B. Combination of running exercise and high dose of anabolic androgenic steroid, nandrolone decanoate, increases protamine deficiency and DNA damage in rat spermatozoa. Andrologia. 2013. doi:10.1111/and.12061. [Epub ahead of print].

44.

Tanaka H, Arakawa H, Yamaguchi T, Shiraishi K, Fukuda S, Matsui K, et al. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature. 2000;404:42–9.PubMedCrossRef

45.

Van den Berg H, Furstner F, van den Bos C, Behrendt H. Decreasing the number of MOPP courses reduces gonadal damage in survivors of childhood Hodgkin disease. Pediatr Blood Cancer. 2004;42:210–5.PubMedCrossRef

46.

Weyermann J, Lochmann D, Zimmer A. A practical note on the use of cytotoxicity assays. Int J Pharm. 2005;288(2):369–76.PubMedCrossRef

47.

Wyns C, Curaba M, Petit S, Vanabelle B, Laurent P, Wese JF, et al. Management of fertility preservation in prepubertal patients: 5 years’ experience at the Catholic University of Louvain. Hum Reprod. 2011;26:737–47.PubMedCrossRef

48.

Wyns C, Curaba M, Vanabelle B, Van Langendonckt A, Donnez J. Options for fertility preservation in prepubertal boys. Hum Reprod Update. 2010;16:312–28.PubMedCrossRef

Titel: Does prepubertal testicular tissue vitrification influence spermatogonial stem cells (SSCs) viability?
verfasst von: Mohammadreza Gholami
Masoud Hemadi
Ghasem Saki
Abolfazl Zendedel
Ali Khodadadi
Javad Mohammadi-asl
Publikationsdatum: 01.10.2013
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 10/2013
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-013-0050-x

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Does prepubertal testicular tissue vitrification influence spermatogonial stem cells (SSCs) viability?