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Journal of Assisted Reproduction and Genetics

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01.10.2013 | Fertility Preservation

Attempted application of bioengineered/biosynthetic supporting matrices with phosphatidylinositol-trisphosphate-enhancing substances to organ culture of human primordial follicles

verfasst von: Galit Lerer-Serfaty, Nivin Samara, Benjamin Fisch, Michal Shachar, Olga Kossover, Dror Seliktar, Avi Ben-Haroush, Ronit Abir

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 10/2013

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Abstract

Purpose

To improve human primordial follicle culture.

Methods

Thin or thick ovarian slices were cultured on alginate scaffolds or in PEG-fibrinogen hydrogels with or without bpV (pic), which prevents the conversion of phosphatidylinositol-trisphosphate (PIP3) to phosphatidylinositol-bisphosphate (PIP2) or 740Y-P which converts PIP2 to PIP3. Follicular growth was evaluated by follicular counts, Ki67 immunohistochemistry, and 17β-estradiol (E2) levels.

Results

BpV (pic) had a destructive effect on cultured follicles. Thawed-uncultured samples had more primordial follicles than samples cultured in basic medium and fewer developing follicles than samples cultured in PEG-fibrinogen hydrogels with 740Y-P. There were more atretic follicles in samples cultured on alginate scaffolds than in PEG-fibrinogen hydrogels, and in samples cultured in PEG-fibrinogen hydrogels with 740Y-P than in PEG-fibrinogen hydrogels with basic medium. Ki67 staining was higher in PEG-fibrinogen hydrogels than on alginate scaffolds. E₂ levels were higher in thick than in thin slices.

Conclusions

PEG-fibrinogen hydrogels appear to have an advantage over alginate scaffolds for culturing human primordial follicles. Folliculogenesis is not increased in the presence of substances that enhance PIP3 production or with thin rather than thick sectioning.

Feigin E, Freud E, Fisch B, Orvieto R, Kravarusic D, Avrahami G, et al. Fertility preservation in female adolescents with malignancies. In: Moorland MT, editor. Cancer in female adolescents. Hauppauge: Nova Science Publishers; 2008. p. 38–103.

Silber SJ. Ovary cryopreservation and transplantation for fertility preservation. Mol Hum Reprod. 2012;18:59–67.PubMedCrossRef

Revel A, Laufer N, Ben Meir A, Lebovich M, Mitrani E. Mirco-organ ovarian transplantation enables pregnancy: a case report. Hum Reprod. 2011;26:1033–97.

Abir R, Nitke S, Ben-Haroush A, Fisch B. In vitro maturation of human primordial ovarian follicles: clinical significance, progress in mammals, and methods for growth evaluation. Histol Histopathol. 2006;21:887–98.PubMed

Abir R, Feinmesser M, Yaniv I, Fisch B, Cohen IJ, Ben-Haroush A, et al. Occasional involvement of the ovary in Ewing sarcoma. Hum Reprod. 2010;25:1708–12.PubMedCrossRef

Telfer EE, McLaughlin M, Ding C, Thong KJ. A two-step serum-free culture system supports development of human oocytes from primordial follicles in the presence of activin. Hum Reprod. 2008;23:1151–8.PubMedCrossRef

Garor R, Abir R, Erman A, Felz C, Nitke S, Fisch B. Effects of basic fibroblast growth factor on in vitro development of human ovarian primordial follicles. Fertil Steril. 2009;91:1967–75.PubMedCrossRef

Kedem A, Fisch B, Garor R, Ben-Zaken A, Gizunterman T. Growth differentiating factor 9 (GDF9) and bone morphogenetic protein 15 both activate development of human primordial follicles in vitro, with seemingly more beneficial effects of GDF9. J Clin Endocrinol Metab. 2011;96:1246–54.CrossRef

Kedem A, Hourvitz A, Fisch B, Shachar M, Cohen S. Alginate scaffold for organ culture of cryopresereved-thawed human ovarian cortical follicles. J Assist Reprod Genet. 2011;28:761–9.PubMedCrossRef

10.

Li J, Kawarmura K, Cheng Y, Liu S, Klein C, Liu S, et al. Activation of dormant follicle to generate mature eggs. PNAS. 2010;107:10280–4.PubMedCrossRef

11.

Kim SR, Lee YC. PTEN as a unique promising therapeutic target for occupational asthma. Immunotoxicology. 2008;30:793–814.CrossRef

12.

John GB, Gallardo TD, Shirley LJ, Castrillon DH. Foxo3 is a PI3K-dependent molecular switch controlling the initiation of oocyte growth. Dev Biol. 2008;321:197–204.PubMedCrossRef

13.

Adhikari D, Gorre N, Risal S, Zhao Z, Zhang H, Shen Y, et al. The safe use of a PTEN inhibitor for the activation of dormant mouse primordial follicles and generation of fertilizable eggs. PLoS One. 2012;7:e39034.PubMedCrossRef

14.

Xu M, West-Farrell ER, Stouffer RL, Shea LD, Woodruff TK, Zelinski MB. Encapsulated three-dimensional culture supports development of nonhuman primate secondary follicles. Biol Reprod. 2009;81:587–94.PubMedCrossRef

15.

Seliktar D. Designing cell-compatible hydrogels for biomedical applications. Science. 2012;336:1124–8.PubMedCrossRef

16.

Dikovskey D, Bianco-Peled H, Seliktar D. Investigating the molecular structure and physical properties of PEG-Fibrinogen hydrogels. Adv Eng Mater. 2010;12:B200–9.CrossRef

17.

Peled E, Boss J, Bejar J, Zinman C, Seliktar D. A novel poly(ethylene glycol)-fibrinogen hydrogel for tibial segmental defect repair in a rat model. J Biomed Mater Res A. 2007;80:874–84.PubMed

18.

Federovich NE, Oudshoorn MH, Van Geemen D, Hennink WE, Alblas J. The effect of photopolymerization on stem cells embedded in hydrogels. Biomaterials. 2009;30:344–53.CrossRef

19.

Bryant SJ, Nuttelman CR, Anseth KS. Cytocompatibility of UV and visible light photoinitiating systems on cultured NIH/3T3 fibroblasts in vitro. J Biomater Sci Polym Ed. 2000;11:439–57.PubMedCrossRef

20.

Elisseeff J, McIntosh W, Anseth K, Riley S, Ragan P, Langer R. Photoencapsulation of chondrocytes in poly(ethylene oxide)-based semi-interpentrating networks. J Biomed Mater Res. 2000;51:164–71.PubMedCrossRef

21.

Hovatta O, Silye R, Abir R, Krausz T, Winston RM. Extracellular matrix improves survival of both stored and fresh human primordial and primary ovarian follicles in long-term culture. Hum Reprod. 1997;12:1032–6.PubMedCrossRef

22.

Blanco-Aparicio C, Renner O, Leal JF, Carnero A. PTEN, more than the AKT pathway. Carcinogenesis. 2007;28:1379–86.PubMedCrossRef

23.

Gougeon A. Regulation of ovarian follicular development in primates: facts and hypotheses. Endocr Rev. 1996;17:55–121.

24.

Gerdes J, Lemke H, Baisch J. Cell cycle analysis of cell proliferation associated human nuclear antigen defined by the monoclonal antibody Ki67. J Immunol. 1984;133:1710–5.PubMed

25.

Scholzen T, Geredes J. The Ki67 protein: from the known and the unknown. J Cell Physiol. 2000;182:311–22.PubMedCrossRef

26.

Rivera OE, Varayoud J, Rodrigues HA, Munoz-de-Toro M, Luque EH. Neonatal exposure to bisphenol A or diethylstilbestrol alters the ovarian follicular dynamics in the lamb. Reprod Toxicol. 2011;32:304–12.PubMedCrossRef

27.

Vendola KA, Zhou J, Adesanya OO, Weil SJ, Bondy CA. Androgens stimulate early stages of follicular growth in the primate ovary. J Clin Invest. 1998;101:2622–9.PubMedCrossRef

28.

Fabbri R, Venturoli S, D'Errico A, Iannascoli C, Gabusi E, Valeri B, et al. Ovarian tissue banking and fertility preservation in cancer patients: histological and immunohistochemical evaluation. Gynecol Oncol. 2003;89:259–66.PubMedCrossRef

29.

Rahmanzadeh R, Hüttmann G, Gerdes J, Scholzen T. Chromophore-assisted light inactivation of pKi67 leads to inhibition of ribosomal RNA synthesis. Cell Prolif. 2007;40:422–30.PubMedCrossRef

30.

David A, Van Langendonckt A, Gilliaux S, Dolmans MM, Donnez J, Amorim CA. Effect of cryopreservation and transplantation on the expression of kit ligand and anti-Mullerian hormone in human ovarian tissue. Hum Reprod. 2012;27:1088–95.PubMedCrossRef

31.

Kleinman HK. Preparation of basement membrane components from EHS tumors. Curr Protoc Cell Biol. 2001;10:10.2.1–10.

32.

Seliktar D, Zisch AH, Lutolf MP, Wrana IJL, Hubbell JA. MMP-2 sensitive, VEGF bearing bioactive hydrogels for promotion of vascular healing. Biomed Mater Res A. 2004;68:704–16.

33.

Xu M, Kreeger PK, Shea LD, Woodruff TK. Tissue-engineered follicles produce live, fertile offspring. Tissue Eng. 2006;12:2739–46.PubMedCrossRef

34.

Shikanov A, Xu M, Woodruff TK, Shea LD. Interpenetrating fibrin-alginate matrices for in vitro ovarian follicle development. Biomaterials. 2009;30:5476–85.PubMedCrossRef

35.

Castrillon DH, Miao L, Kollipara R, Horner JW, DePinho RA. Suppression of ovarian follicle activation in mice by the transcription factor Foxo3a. Science. 2003;301:215–8.PubMedCrossRef

36.

Kagawa N, Sliber S, Kuwayama M. Successful vitrification of bovine and human ovarian tissue. Reprod Biomed Online. 2009;18:568–77.PubMedCrossRef

37.

Jin SY, Lei L, Shikanov A, Shea LD, Woodruff TK. A novel two-step strategy for in vitro culture of early-stage ovarian follicles in the mouse. Fertil Steril. 2010;93:2633–9.PubMedCrossRef

38.

O'Brien MJ, Pendola JK, Eppig JJ. A revised protocol for in vitro development of mouse oocytes from primordial follicles dramatically improves their developmental competence. Biol Reprod. 2003;68:1682–6.PubMedCrossRef

Titel: Attempted application of bioengineered/biosynthetic supporting matrices with phosphatidylinositol-trisphosphate-enhancing substances to organ culture of human primordial follicles
verfasst von: Galit Lerer-Serfaty
Nivin Samara
Benjamin Fisch
Michal Shachar
Olga Kossover
Dror Seliktar
Avi Ben-Haroush
Ronit Abir
Publikationsdatum: 01.10.2013
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 10/2013
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-013-0052-8

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Attempted application of bioengineered/biosynthetic supporting matrices with phosphatidylinositol-trisphosphate-enhancing substances to organ culture of human primordial follicles