Background
Methods
Plant material and extraction
Reagents and equipment
Animals
Scald wound induction and treatment
Gross examination of the scald wound lesions
Collection and processing of serum and scald wound tissue
Analysis of cytokine and angiogenesis factors
Histopathological studies with hematoxylin and eosin and Masson-Goldner trichrome stain
Preliminary phytochemical screening
Statistical analysis
Results and discussion
Gross examination
Measurement of scald wound size
Histopathological examination
Day | Epithelialization | ||||
---|---|---|---|---|---|
CON | SSD | Vaseline | 5 % AJE | 20 % AJE | |
2 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.3 ± 0.3 | 0. 00 ± 0.00 | 0.1 ± 0.1 |
14 | 1.5 ± 0.4 | 2.3 ± 0.07 | 2.9 ± 0.1** | 2.9 ± 0.1*** | 3.0 ± 0.0** |
21 | 1.0 ± 0.0 | 2.7 ± 0.18*** | 2.9 ± 0.1*** | 2.9 ± 0.1*** | 3.0 ± 0.0*** |
Vascularization | |||||
2 | 0.0 ± 0.0 | 0.0 ± 0.0# | 1.6 ± 0.6* | 1.8 ± 0.3* | 1.6 ± 0.4* |
14 | 1.2 ± 0.4 | 1.7 ± 0.4 | 1.9 ± 0.1 | 2.2 ± 0.2 | 2.2 ± 0.2 |
21 | 1.5 ± 0.2 | 1.7 ± 0.1 | 1.9 ± 0.2 | 2.3 ± 0.3 | 2.5 ± 0.1* |
Granulation | |||||
2 | 0.0 ± 0.0 | 0.6 ± 0.1* | 1.2 ± 0.0*** | 1.5 ± 0.0*** | 1.3 ± 0.1*** |
14 | 1.4 ± 0.2 | 1.9 ± 0.1 | 2.0 ± 0.1* | 2.6 ± 0.1***,# | 2.8 ± 0.2***,# |
21 | 2.8 ± 0.0 | 2.8 ± 0.1 | 2.9 ± 0.1 | 2.9 ± 0.1 | 3.0 ± 0.1 |
Inflammation | |||||
2 | 2.6 ± 0.2 | 2.0 ± 0.1 | 2.9 ± 0.1 | 2.8 ± 0.2 | 2.7 ± 0.1 |
14 | 2.9 ± 0.1 | 2.3 ± 0.2 | 2.3 ± 0.1* | 1.3 ± 0.2***,### | 1.2 ± 0.1***, ## |
21 | 2.3 ± 0.3 | 1.3 ± 0.1* | 1.3 ± 0.1* | 0.9 ± 0.1** | 0.9 ± 0.3** |
Collagen deposition | |||||
2 | 1.2 ± 0.1 | 1.1 ± 0.2 | 1.5 ± 0.1 | 1.3 ± 0.2 | 1.1 ± 0.2 |
14 | 2.2 ± 0.2 | 2.5 ± 0.2 | 2.7 ± 0.1 | 2.8 ± 0.1* | 2.9 ± 0.1** |
21 | 2.3 ± 0.2 | 2.6 ± 0.1 | 2.7 ± 0.2 | 2.9 ± 0.1* | 3.0 ± 0.1* |
Total score | |||||
2 | 1.0 ± 0.0 | 1.4 ± 0.1 | 1.2 ± 0.0 | 1.4 ± 0.1 | 1.5 ± 0.2 |
14 | 2.1 ± 0.1 | 2.5 ± 0.1 | 2.7 ± 0.1** | 3.3 ± 0.2***, # | 3.0 ± 0.1*** |
21 | 2.4 ± 0.0 | 3.2 ± 0.1** | 3.3 ± 0.1*** | 3.6 ± 0.1*** | 3.9 ± 0.1***, # # |