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20.05.2020 | Original articles

Effect of Ginkgo biloba extract on pacemaker channels encoded by HCN gene

verfasst von: Hui Chen, MD PhD, Yongjun Chen, MD PhD, Jing Yang, MD PhD, Pan Wu, MD PhD, Xin Wang, MD PhD, Congxin Huang, MD PhD

Erschienen in: Herz | Ausgabe 3/2021

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Abstract

Background

In the present study, the electropharmacological activity of traditional Chinese medicine, Ginkgo biloba extract (GBE), on human hyperpolarization-activated nucleotide-gated (HCN) channels and the underlying “funny” currents was investigated.

Methods

Standard two-electrode voltage-clamp recordings were employed to examine the properties of cloned HCN subunit currents expressed in Xenopus oocytes under controlled conditions and GBE administration.

Results

We found that GBE irreversibly inhibited the HCN2 and HCN4 channel currents in a concentration-dependent fashion and that the HCN4 current was more sensitive to GBE compared with HCN2. In addition, GBE inhibition of the current amplitudes of HCN2 and HCN4 currents was accompanied by a decrease in the activation and deactivation kinetics.

Conclusion

The results of this study contribute toward illustrating the antiarrhythmic mechanism of GBE, which might be useful for the treatment of arrhythmia.

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Titel: Effect of Ginkgo biloba extract on pacemaker channels encoded by HCN gene
verfasst von: Hui Chen, MD PhD
Yongjun Chen, MD PhD
Jing Yang, MD PhD
Pan Wu, MD PhD
Xin Wang, MD PhD
Congxin Huang, MD PhD
Publikationsdatum: 20.05.2020
Verlag: Springer Medizin
Erschienen in: Herz / Ausgabe 3/2021
Print ISSN: 0340-9937
Elektronische ISSN: 1615-6692
DOI: https://doi.org/10.1007/s00059-020-04933-z

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Effect of Ginkgo biloba extract on pacemaker channels encoded by HCN gene