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Erschienen in: Journal of Medical Ultrasonics 2/2019

18.01.2019 | Original Article

Effects of Clematis chinensis Osbeck mediated by low-intensity pulsed ultrasound on transforming growth factor-β/Smad signaling in rabbit articular chondrocytes

verfasst von: Ya-lan Pan, Yong Ma, Yang Guo, Juan Tu, Ge-pu Guo, Shendu Marshall Ma, Su-yang Zheng, Peng-cheng Tu, Bei-bei Yu, Gui-cheng Huang

Erschienen in: Journal of Medical Ultrasonics | Ausgabe 2/2019

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Abstract

Purpose

Clematis chinensis Osbeck (CCO) is an essential herb that has been shown to promote the biological functions of cartilage cells. In this study, we aimed to explore whether and how low-intensity pulsed ultrasound (LIPUS) enhanced CCO delivery into chondrocytes and stimulated biological activity in vitro.

Methods

Chondrocytes were isolated from knee articular cartilage of 2-week-old rabbits and treated with LIPUS plus CCO or recombinant transforming growth factor beta 1 (TGF-β1; 0.5 ng/mL), with or without anti-TGF-β1 antibodies (10 μg/mL), for 3 days. Cell proliferation was assessed by Cell-Counting Kit-8 assays. Immunocytochemistry, western blotting, and quantitative polymerase chain reaction were applied to detect the expression of type II collagen and some molecules in the TGF-β1 signal pathway.

Results

LIPUS plus 0.1 mg/mL CCO solution promoted chondrocyte proliferation and type II collagen and TGF-β1 expression synergistically in vitro (P < 0.05). In addition, treatment with anti-TGF-β1 antibodies blocked this effect (P < 0.01), but not completely. CCO plus LIPUS also showed more enhanced effects on promoting TGF-β receptor II and Smad2 signaling and reducing Smad7 signaling than either intervention separately (P < 0.05).

Conclusions

CCO plus LIPUS promoted extracellular matrix deposition by accelerating the TGF-β/Smad-signaling pathway in chondrocytes.
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Metadaten
Titel
Effects of Clematis chinensis Osbeck mediated by low-intensity pulsed ultrasound on transforming growth factor-β/Smad signaling in rabbit articular chondrocytes
verfasst von
Ya-lan Pan
Yong Ma
Yang Guo
Juan Tu
Ge-pu Guo
Shendu Marshall Ma
Su-yang Zheng
Peng-cheng Tu
Bei-bei Yu
Gui-cheng Huang
Publikationsdatum
18.01.2019
Verlag
Springer Singapore
Erschienen in
Journal of Medical Ultrasonics / Ausgabe 2/2019
Print ISSN: 1346-4523
Elektronische ISSN: 1613-2254
DOI
https://doi.org/10.1007/s10396-018-0920-z

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