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Journal of Assisted Reproduction and Genetics
Erschienen in: Journal of Assisted Reproduction and Genetics 7/2011

01.07.2011 | Fertility Preservation

Effects of cooling rates and ice-seeding temperatures on the cryopreservation of whole ovaries

verfasst von: Jian-Min Zhang, Yan Sheng, Yong-Zhi Cao, Hong-Yan Wang, Zi-Jiang Chen

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 7/2011

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Abstract

Purpose

The aim of this study was to detect the effects of different cooling rates and different ice-seeding temperatures on the cryopreservation of whole ovaries.

Methods

Cow whole ovaries were slowly frozen using different protocols with different cooling rates and different ice-seeding temperatures. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles and the follicular densities of grafts were measured.

Results

Protocol IIb was most effective protocol. Protocol Ib was more effective than protocol Ia and protocol Ic, and protocol IIIb was more effective than protocol IIIa and protocol IIIc.

Conclusions

Protocol IIb (the cooling rate was 0.2°C/min, and the ice-seeding temperature was −5°C) was appropriate for slow freezing of cow whole ovaries.
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Metadaten
Titel
Effects of cooling rates and ice-seeding temperatures on the cryopreservation of whole ovaries
verfasst von
Jian-Min Zhang
Yan Sheng
Yong-Zhi Cao
Hong-Yan Wang
Zi-Jiang Chen
Publikationsdatum
01.07.2011
Verlag
Springer US
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 7/2011
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-011-9557-1

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