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Digestive Diseases and Sciences

Erschienen in:

01.10.2007 | Original Paper

Effects of Nanocrystalline Silver (NPI 32101) in a Rat Model of Ulcerative Colitis

verfasst von: Kailash C. Bhol, Paul J. Schechter

Erschienen in: Digestive Diseases and Sciences | Ausgabe 10/2007

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Abstract

Nanocrystalline silver (NPI 32101) has been demonstrated to have antimicrobial and anti-inflammatory properties. The purpose of this study was to assess the effect of NPI 32101 in a rat model of ulcerative colitis and the possible mechanisms of action of the effects observed. NPI 32101, 4 mg/kg intracolonically or 40 mg/kg orally, significantly reduced colonic inflammation compared to the placebo and no-treatment groups. Sulfasalazine (100 mg/kg), either intracolonically or orally, also reduced colonic inflammation. NPI 32101 significantly suppressed the expression of matrix metalloproteinase (MMP)-9, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-12, whereas sulfasalazine suppressed MMP-9, IL-1β, and TNF-α, but not IL-12, compared to placebo. MMP-9 activity was reduced by NPI 32101 and sulfasalazine. NPI 32101 administered intracolonically or orally decreases ulcerative colitis in a rat model and is as effective as sulfasalazine. NPI 32101 treatment suppresses the expression and activity of MMP-9 and the expression of TNF-α, IL-1β, and IL-12, mechanisms by which NPI 32101 may exert its anti-inflammatory effects. NPI 32101 may have therapeutic potential for treatment of ulcerative colitis.

Podolsky DK (2002) Inflammatory bowel disease. N Eng J Med 347:417–429CrossRef

Louis E (2001) The immuno-inflammatory reaction in Crohn’s disease and ulcerative colitis: characterization, genetics and clinical application: focus on TNF-alpha. Acta Gastroenterol 64:1–5

Reinecker HC, Steffen M, Witthoeft T, Pflueger I, Schreiber S, MacDermott RP (1993) Enhanced secretion of TNF-alpha, IL-6, and IL-1 by isolated lamina propria mononuclear cells from patients with ulcerative colitis and Crohn’s disease. Clin Exp Immunol 94:174–181PubMedCrossRef

Monteleone G, Biancone L, Marasco R, Morrone G, Marasco O, Luzza F, Pallone F (1997) Interleukin-12 is expressed and actively released by Crohn’s disease intestinal lamina propria mononuclear cells. Gastroenterology 112:1169–1178PubMedCrossRef

Bauditz J, Wedel S, Lochs H (2002) Thalidomide reduces tumor necrosis factor alpha and interleukin-12 production in patients with chronic active Crohn’s disease. Gut 50:196–200PubMedCrossRef

Seegers D, Bouna G, Pena AS (2002) A critical approach to new forms of treatments of Crohns diseases and ulcerative colitis. Aliment Pharmacol 16:53–58CrossRef

Ogata H, Hibi T (2003) Cytokine and anti-cytokine therapies for inflammatory bowel diseases. Curr Pharm Des 14:1107–1113CrossRef

Schmidt C, Marth T, Wittig BM (2002) Interleukin-12 antagonists as new therapeutic agents in inflammatory bowel disease. Pathobiology 70:177–183PubMedCrossRef

Su C, Salazberg BA, Lewis JD, Deren JJ, Kornbluth A, Katzka DA, Stein RB, Adler DR, Lichtenstein GR (2002) Efficacy of anti-tumor necrosis factor therapy in patients with ulcerative colitis. Am J Gastroenterol 97:2577–2584PubMedCrossRef

10.

Yin H Q, Langford R, Burrell RE (1999) Comparative evaluation of the antimicrobial activity of Acticoat antimicrobial dressing. J Burn Care Rehab 20:195–200CrossRef

11.

Bhol KC, Alroy J, Schechter PJ (2004) Anti-inflammatory effect of topical nanocrystalline silver cream on allergic contact dermatitis in a guinea pig model. Clin Exp Dermatol 29:282–287PubMedCrossRef

12.

Bhol KC, Schechter PJ (2005) Topical nanocrystalline silver cream suppresses inflammatory cytokines and induces apoptosis of inflammatory cells in a murine model of allergic contact dermatitis. Br J Dermatol 152:1235–1242PubMedCrossRef

13.

Bhol K C, Schechter PJ (2005) Topical nanocrystalline silver cream inhibits expression of MMP-9 in animal models of allergic contact dermatitis. J Invest Dermatol (Suppl) 124:A117

14.

Castaneda FE, Walia B, Kumar MV, Patel NR, Roser S, Kolachala L, Rojas M, Wang L, Opera G, Garg P, Gewirtz AT, Roman J, Merlin D, Sitaraman SV (2005) Targeted deletion of Metalloproteinase 9 attenuates experimental colitis in mice: central role of epithelial derived MMP. Gastroenterology 129:1991–2008PubMedCrossRef

15.

Marco RD, Mangano K, Quattrocchi C, Musumeci R, Speciale AM, Papaccio G, Buschard K, Bendtzen K, Nicoletti F (2003) Curative effects of sodium fusidate on the development of dinitrobenzenesulfonic acid-induced colitis in rats. Clin Immunol 109:266–271PubMedCrossRef

16.

Cuzzocrea S, McDonald MC, Mazzon E, Mota-Filipe H, Centorrino T, Terranova ML, Ciccolo A, Britti D, Caputi AP, Thiemermann C (2001) Calpain inhibitor 1 reduces colon injury caused by dinitrobenzene sulphonic acid in the rat. Gut 48:478–488PubMedCrossRef

17.

Kim YS, Son M, Ko JI, Cho H, Yoo M, Kim WB, Song SI, Kim CY (1999) Effect of DA-6034 a derivative of Flanoid on experimental animal model of inflammatory bowel diseases. Arch Pharm Res 22:354–360PubMedCrossRef

18.

Morris GP, Beck PL, Herridge MS, Depew WT, Szewezuk MR, Wallace JL (1989) Hapten-induced model of chronic inflammation and ulceration in the rat colon. Gastroenterology 96:795–803PubMed

19.

Luke H H, Ko JKS, Fung HS, Cho CH (2002) Delineation of the protective action of zinc sulfate on ulcerative colitis. Eur J Pharmacol 443:197–204CrossRef

20.

Wallace JL, MacNaughton WK, Morris GP, Beck PL (1989) Inhibition of leukotrine synthesis markedly accelerate healing in a rat model of inflammatory bowel disease. Gastroenterology 96:29–36PubMed

21.

Griffiths CEM, Barker JNWN, Kunkel S, Nickoloff BJ (1991) Modulation of leukocyte adhesion molecules, a T-cell chemotaxin (IL-8) and a regulatory cytokine (TNF-a) in allergic contact dermatitis (rhus dermatitis). Br J Dermatol 124:519–526PubMedCrossRef

22.

Hawkins JV, Emmel EL, Feuer JJ (1997) Protease activity in a hapten-induced model of ulcerative colitis in rats. Dig Dis Sci 42:1969–1980PubMedCrossRef

23.

Medina C, Videla S, Radomski A, Radomski MW, Antollin M, Guarner F, Vilaseca J, Salas A, Malagelada JR (2003) Increased activity and expression of matrix metalloproteinase-9 in a rat model of distal colitis. Am J Physiol Gastrointest Liver Physiol 284:G116–G122PubMed

24.

Hanauer SB, Present DH (2003) The state of the art in the management of inflammatory bowel disease. Rev Gastroenterol Disord 3:81–92PubMed

25.

Doering J, Begue B, Lentze MJ (2004) Induction of T lymphocyte apoptosis by sulfasalazine in patients with Crohn’s disease. Gut 53:1632–1638PubMedCrossRef

26.

Azad Khan AK, Truelove SC, Aronseq JK (1982) The disposition and metabolism of sulfasalazine (salicylazosulfapyridine) in man. Br J Clin Pharmacol 13:523–528

27.

Bertevello PL, Logullo AF, Fabio SN, Campos FM, Chiferi V, Alves CC, Torrinhas RS, Gama-Rodrigues JJ, Waitzberg DL (2005) Immunohistochemical assessment of mucosal cytokine profile in acetic acid experimental colitis. Clinics 60:277–286PubMedCrossRef

28.

Sartor RB (1994) Cytokines in intestinal inflammation: pathophysiological and clinical considerations. Gastroenterology 106:533–536PubMed

29.

Blam ME, Stein RBS, Lichtenstein GR (2001) Integrating anti-tumor necrosis factor therapy in inflammatory bowel disease: current and future perspectives. Am J Gastroenterol 96:1977–1997PubMed

30.

Rogler G, Andus T (1998) Cytokines in inflammatory bowel disease. World J Surg 22:382–389PubMedCrossRef

31.

Neurath MF, Fuss I, Kelsall BL, Stuber E, Strober W (1995) Antibodies to interleukin 12 abrogate established experimental colitis in mice. J Exp Med 182:1281–1290PubMedCrossRef

32.

Krane SM (1994) Clinical importance of matrix metalloproteinase and their inhibitors. Ann NY Acad Sci 732:1–10PubMedCrossRef

33.

von Lampe B, Barthel B, Coupland SE, Riecken EO, Rosewicz S (2000) Differential expression of matrix metalloproteinases and their tissue inhibitors in colon mucosa of patients with inflammatory bowel diseases. Gut 47:63–73PubMedCrossRef

34.

Seifert WF, Wobbes T, Hendriks T (1996) Divergent patterns of matrix metalloproteinase activity during wound healing in ileum and colon of rats. Gut 39:114–119PubMed

35.

Wright BJ, Lam K, Burrell AG, Olson ME, Burrell RE (2002) Early healing events in a porcine model of contaminated wounds: effect of nanocrystalline silver on matrix metalloproteinases, cell apoptosis and healing. Wound Rep Reg 10:141–151CrossRef

Titel: Effects of Nanocrystalline Silver (NPI 32101) in a Rat Model of Ulcerative Colitis
verfasst von: Kailash C. Bhol
Paul J. Schechter
Publikationsdatum: 01.10.2007
Verlag: Springer US
Erschienen in: Digestive Diseases and Sciences / Ausgabe 10/2007
Print ISSN: 0163-2116
Elektronische ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-006-9738-4

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