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10.12.2019 | Original Article

Effects of phosphorylated estrogen receptor alpha on apoptosis in human endometrial epithelial cells

verfasst von: Shunsuke Uchida, Mierxiati Saimi, Zhong-Lian Li, Hidenobu Miyaso, Kenta Nagahori, Shinichi Kawata, Takuya Omotehara, Yuki Ogawa, Masahiro Itoh

Erschienen in: Anatomical Science International | Ausgabe 2/2020

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Abstract

It is known that the activities of estrogen receptor α (ERα) can be modulated by epidermal growth factor (EGF) through the phosphatidylinostitol 3-kinase-alpha serine/threonine protein kinase (PI3K-AKT) pathway by phosphorylation. To clarify how ERα functions are regulated in endometrial cells during menstrual cycle, molecules related to phosphorylation of ERα (pERα) were examined. It was found that the expression of phosphorylated AKT on serine 473 (pAKT-Ser473) was increased during the proliferative phase, but decreased in the secretory phase. Although the expression of pAKT on threonine 308 in the proliferative phase was only identified in the wall of arterioles, it was strongly expressed in the cytoplasm of endometrial glandular cells after entering the secretory phase. Further observations revealed that while the expression of pERα-Ser104 was constant, pERα-Ser118 was expressed following a cyclic pattern similar to that of the pAKT-Ser473. Following treatment with specific inhibitors for EGFR-PI3K-AKT pathway, it was found that while the expression of pERα-Ser118 and pERα-Ser167 was inhibited, the induced apoptosis could be antagonized by the addition of estrogen, indicating that a mitochondrial pathway is involved. Therefore, pAKT and pERα or ERα could act cooperatively on coiled arterioles and endometrial cells in order to control menstrual cycle.

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Titel: Effects of phosphorylated estrogen receptor alpha on apoptosis in human endometrial epithelial cells
verfasst von: Shunsuke Uchida
Mierxiati Saimi
Zhong-Lian Li
Hidenobu Miyaso
Kenta Nagahori
Shinichi Kawata
Takuya Omotehara
Yuki Ogawa
Masahiro Itoh
Publikationsdatum: 10.12.2019
Verlag: Springer Singapore
Erschienen in: Anatomical Science International / Ausgabe 2/2020
Print ISSN: 1447-6959
Elektronische ISSN: 1447-073X
DOI: https://doi.org/10.1007/s12565-019-00515-0

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Effects of phosphorylated estrogen receptor alpha on apoptosis in human endometrial epithelial cells

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