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Erschienen in:
23.03.2016 | Erratum
Erratum to: Estrogen receptor beta binds Sp1 and recruits a corepressor complex to the estrogen receptor alpha gene promoter
Erschienen in: Breast Cancer Research and Treatment | Ausgabe 2/2016
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Uunfortunately, authors could not find the original film from which the figure was drawn. Therefore, as suggested by the Editor, they have repeated the relative experiment, and ask to publish this new figure as a correction. The authors apologize for any inconvenience that it may cause.
Fig. 3
ER beta recruits corepressors to the Sp-1 site in the ER alpha gene promoter. a Nuclear extracts from MCF-7 cells transfected with either empty vector or ER beta expression plasmid were incubated with a double-stranded Sp-1 specific sequence probe labeled with [γ32P] ATP and subjected to elettrophoresis in a 6 % polyacrylamide gel (lanes 1 and 4). Competition experiments were performed adding as competitor a 100-fold molar excess of unlabeled probe (lanes 2 and 11) or a 100-fold molar excess of unlabeled oligonucleotide containing a mutated Sp-1 motif (lane 3). Nuclear extracts from MCF-7 over-expressing ER beta were incubated with anti-NCoR (lane 5) or anti-SMRT (lane 6) or anti-Sp-1 (lane 7) or anti-ER beta (lane 8) or IgG (lane 9) antibodies, in the presence of the probe. Lane 10 Sp-1 protein. Lane 12 probe alone
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