Fig. 3
RAGE binding to S100A8/A9-induced EMT through the NF-κB signaling pathway. a The expression of E-cadherin and vimentin in Scr/MDA231, SiRAGE/MDA231, MCF-7/con, and MCF-7/RAGE cells with 10 μg/mL rS100A8/A9 at different time points. β-actin was used as a loading control. Quantification of relative protein levels on three different western blots is shown below the blots. b Expression of epithelial markers, E-cadherin, as well as mesenchymal markers, N-cadherin and vimentin, was examined by western blot in Scr/MDA231, SiRAGE/MDA231, MCF-7/con, and MCF-7/RAGE cells with or without 10 μg/mL rS100A8/A9 for 48 h. β-actin was used as a loading control. Quantification of relative protein levels on three different western blots is shown below the blots. c Fluorescence microscopic staining of E-cadherin, N-cadherin, and vimentin (green) is indicated in the Scr/MDA231, SiRAGE/MDA231, MCF-7/Con, and MCF-7/RAGE cells with rS100A8/A9 for 48 h. Nuclear DNA was stained with DAPI (blue). Scale bar 20 μm. Data were collected in this set of figures from a representative of at least three independent experiments