nach oben

Erschienen in:

15.04.2023 | Original Article

Evaluation of applicability of micro-segmental analysis to hair treated with heat and haircare products

verfasst von: Kenji Kuwayama, Hajime Miyaguchi, Tatsuyuki Kanamori, Kenji Tsujikawa, Tadashi Yamamuro, Hiroki Segawa, Yuki Okada, Yuko T. Iwata

Erschienen in: Forensic Toxicology | Ausgabe 2/2023

Einloggen, um Zugang zu erhalten

Abstract

Purpose

Micro-segmental analysis (MSA), which enables the measurement of detailed drug distributions in hair by segmenting a single hair strand at 0.4 mm intervals, is indispensable for estimating the day of drug ingestion. However, haircare with dryers and various products can influence drug concentrations in hair. Therefore, the applicability of MSA to hair that was treated with heat or various haircare products was evaluated.

Methods

Reference hair strands containing drugs consistently along the hair shafts were collected from patients who ingested four hay-fever medicines (fexofenadine, epinastine, cetirizine, and loratadine) daily for 4 months. The hair strands were divided into eight 4 mm regions from the proximal end, and each region was placed on an electric hot plate at 100–200 °C or soaked in haircare products, such as shampoo and bleaching agent. The hair regions were subjected to MSA. Moreover, after a patient was administered midazolam at a single dose and the hair was bleached, the day of midazolam administration was estimated using MSA.

Results

Repetitive heating for 1 min and daily haircare products, such as shampoo, hardly affected the drugs in hair, whereas bleaching products containing H₂O₂ decreased the amounts of hay-fever medicines in the hair up to 58%. However, the amount of midazolam did not decrease in bleached hair and the day of midazolam administration was successfully estimated.

Conclusions

The analytes used in this study were minimally affected by ordinary haircare and could be detected even in bleached hair. Therefore, MSA can be applicable regardless of haircare history.

Nur mit Berechtigung zugänglich

Uematsu T, Nakano M, Akiyama H, Nakashima M (1993) The measurement of a new antimicrobial quinolone in hair as an index of drug exposure. Br J Clin Pharmacol 35:199–203. https://doi.org/10.1111/j.1365-2125.1993.tb05686.x(openaccessarticle)CrossRefPubMedPubMedCentral

Johansen SS, Dahl-Sørensen R (2012) A drug rape case involving triazolam detected in hair and urine. Int J Legal Med 126:637–643. https://doi.org/10.1007/s00414-011-0654-6CrossRefPubMed

Kim H, Lee S, In S, Park M, Cho S, Shin J, Lee H, Han E (2018) The correlation between concentrations of zolpidem and benzodiazepines in segmental hair samples and use patterns. Forensic Sci Int 282:13–23. https://doi.org/10.1016/j.forsciint.2017.10.044CrossRefPubMed

Xiang P, Sun Q, Shen B, Chen P, Liu W, Shen M (2011) Segmental hair analysis using liquid chromatography-tandem mass spectrometry after a single dose of benzodiazepines. Forensic Sci Int 204:19–26. https://doi.org/10.1016/j.forsciint.2010.04.046CrossRefPubMed

Kuwayama K, Miyaguchi H, Iwata YT, Kanamori T, Tsujikawa K, Yamamuro T, Segawa H, Inoue H (2016) Three-step drug extraction from a single sub-millimeter segment of hair and nail to determine the exact day of drug intake. Anal Chim Acta 948:40–47. https://doi.org/10.1016/j.aca.2016.10.029CrossRefPubMed

Kuwayama K, Miyaguchi H, Kanamori T, Tsujikawa K, Yamamuro T, Segawa H, Okada Y, Iwata YT (2022) Micro-segmental hair analysis: detailed procedures and applications in forensic toxicology. Forensic Toxicol 40:215–233. https://doi.org/10.1007/s11419-022-00619-9CrossRefPubMedPubMedCentral

Kintz P, Salomone A, Vincenti M (eds) (2015). Academic Press, Cambridge. https://doi.org/10.1016/C2014-0-00832-5CrossRef

Pecoraro V, Astore IPL (1990) Measurements of hair growth under physiological conditions. In: Orfanos CE, Happle R (eds) Hair and hair diseases. Springer, Berlin. https://doi.org/10.1007/978-3-642-74612-3_10CrossRef

Kuwayama K, Nariai M, Miyaguchi H, Iwata YT, Kanamori T, Tsujikawa K, Yamamuro T, Segawa H, Abe H, Iwase H, Inoue H (2018) Accurate estimation of drug intake day by microsegmental analysis of a strand of hair using internal temporal markers. J Appl Lab Med 3:37–47. https://doi.org/10.1373/jalm.2017.025346(openaccessarticle)CrossRefPubMed

10.

Kuwayama K, Nariai M, Miyaguchi H, Iwata YT, Kanamori T, Tsujikawa K, Yamamuro T, Segawa H, Abe H, Iwase H, Inoue H (2018) Micro-segmental hair analysis for proving drug-facilitated crimes: evidence that a victim ingested a sleeping aid, diphenhydramine, on a specific day. Forensic Sci Int 288:23–28. https://doi.org/10.1016/j.forsciint.2018.04.027CrossRefPubMed

11.

Kuwayama K, Miyaguchi H, Iwata YT, Kanamori T, Tsujikawa K, Yamamuro T, Segawa H, Inoue H (2019) Strong evidence of drug-facilitated crimes by hair analysis using LC–MS/MS after micro-segmentation. Forensic Toxicol 37:480–487. https://doi.org/10.1007/s11419-019-00472-3CrossRef

12.

Kuwayama K, Miyaguchi H, Kanamori T, Tsujikawa K, Yamamuro T, Segawa H, Okada Y, Iwata YT (2021) Development of an improved method to estimate the days of continuous drug ingestion, based on the micro-segmental hair analysis. Drug Test Anal 13:1295–1304. https://doi.org/10.1002/dta.3025CrossRefPubMed

13.

Kuwayama K, Miyaguchi H, Kanamori T, Tsujikawa K, Yamamuro T, Segawa H, Okada Y, Iwata YT (2023) Possibility of drug-distribution measurement in the hair of drowned bodies: evaluation of drug stability in water-soaked hair using micro-segmental analysis. Int J Legal Med 137:89–98. https://doi.org/10.1007/s00414-022-02900-3CrossRefPubMed

14.

Cirimele V, Kintz P, Mangin P (1995) Drug concentrations in human hair after bleaching. J Anal Toxicol 19:331–332. https://doi.org/10.1093/jat/19.5.331CrossRefPubMed

15.

Pötsch L, Skopp G (1996) Stability of opiates in hair fibers after exposure to cosmetic treatment. Forensic Sci Int 81:95–102. https://doi.org/10.1016/s0379-0738(96)01974-3CrossRefPubMed

16.

Skopp G, Pötsch L, Moeller MR (1997) On cosmetically treated hair—aspects and pitfalls of interpretation. Forensic Sci Int 84:43–52. https://doi.org/10.1016/s0379-0738(96)02047-6CrossRefPubMed

17.

Jurado C, Kintz P, Menéndez M, Repetto M (1997) Influence of the cosmetic treatment of hair on drug testing. Int J Legal Med 110:159–163. https://doi.org/10.1007/s004140050056CrossRefPubMed

18.

Yegles M, Marson Y, Wennig R (2000) Influence of bleaching on stability of benzodiazepines in hair. Forensic Sci Int 107:87–92. https://doi.org/10.1016/s0379-0738(99)00152-8CrossRefPubMed

19.

Scientific Working Group for Forensic Toxicology (2013) Scientific Working Group for Forensic Toxicology (SWGTOX) standard practices for method validation in forensic toxicology. J Anal Toxicol 37:452–474. https://doi.org/10.1093/jat/bkt054CrossRef

20.

Simons FE, Simons KJ (1999) Clinical pharmacology of new histamine H1 receptor antagonists. Clin Pharmacokinet 36:329–352. https://doi.org/10.2165/00003088-199936050-00003CrossRefPubMed

21.

Kamata T, Shima N, Miki A, Matsuo E, Yamamoto T, Tsuchihashi H, Sato T, Shimma S, Katagi M (2020) High spatial-resolution matrix-assisted laser desorption/ionization-ion trap-time-of-flight tandem mass spectrometry imaging for depicting longitudinal and transverse distribution of drugs incorporated into hair. Anal Chem 92:5821–5829. https://doi.org/10.1021/acs.analchem.9b05401CrossRefPubMed

22.

Ettlinger J, Kirchen L, Yegles M (2014) Influence of thermal hair straightening on ethyl glucuronide content in hair. Drug Test Anal 6:74–77. https://doi.org/10.1002/dta.1648CrossRefPubMed

23.

Ramírez Fernández MDM, Wille SMR, Di Fazio V, Samyn N (2019) Influence of bleaching and thermal straightening on endogenous GHB concentrations in hair: an in vitro experiment. Forensic Sci Int 297:277–283. https://doi.org/10.1016/j.forsciint.2019.02.027CrossRefPubMed

24.

Gerace E, Veronesi A, Martra G, Salomone A, Vincenti M (2017) Study of cocaine incorporation in hair damaged by cosmetic treatments. Forensic Chem 3:69–73. https://doi.org/10.1016/j.forc.2017.02.004CrossRef

25.

Smith RAW, Garrett B, Naqvi KR, Fülöp A, Godfrey SP, Marsh JM, Chechik V (2017) Mechanistic insights into the bleaching of melanin by alkaline hydrogen peroxide. Free Radic Biol Med 108:110–117. https://doi.org/10.1016/j.freeradbiomed.2017.03.014CrossRefPubMed

26.

Petzel-Witt S, Pogoda W, Wunder C, Paulke A, Schubert-Zsilavecz M, Toennes SW (2018) Influence of bleaching and coloring on ethyl glucuronide content in human hair. Drug Test Anal 10:177–183. https://doi.org/10.1002/dta.2206CrossRefPubMed

27.

Van Elsué N, Yegles M (2019) Influence of cosmetic hair treatments on cannabinoids in hair: bleaching, perming and permanent coloring. Forensic Sci Int 297:270–276. https://doi.org/10.1016/j.forsciint.2019.02.030CrossRefPubMed

28.

He X, Wang JF, Wang Y (2021) Influence of cosmetic hair treatments on hair of methamphetamine abuser: bleaching, perming and coloring. Ecotoxicol Environ Saf 222:112542. https://doi.org/10.1016/j.ecoenv.2021.112542CrossRefPubMed

29.

Miyaguchi H, Kuwayama K (2015) Comparison of sample preparation methods for zolpidem extraction from hair. Forensic Toxicol 33:159–164. https://doi.org/10.1007/s11419-014-0256-3CrossRef

30.

Yu H, Jang W-J, Jang J-H, Park B, Seo Y-H, Jeong C-H, Lee S (2017) Role of hair pigmentation in drug incorporation into hair. Forensic Sci Int 281:171–175. https://doi.org/10.1016/j.forsciint.2017.11.004CrossRefPubMed

31.

Villain M, Chèze M, Tracqui A, Ludes B, Kintz P (2004) Windows of detection of zolpidem in urine and hair: application to two drug facilitated sexual assaults. Forensic Sci Int 143:157–161. https://doi.org/10.1016/j.forsciint.2004.04.019CrossRefPubMed

32.

Cui X, Xiang P, Zhang J, Shi Y, Shen B, Shen M (2013) Segmental hair analysis after a single dose of zolpidem: comparison with a previous study. J Anal Toxicol 37:369–375. https://doi.org/10.1093/jat/bkt035(openaccessarticle)CrossRefPubMed

Titel: Evaluation of applicability of micro-segmental analysis to hair treated with heat and haircare products
verfasst von: Kenji Kuwayama
Hajime Miyaguchi
Tatsuyuki Kanamori
Kenji Tsujikawa
Tadashi Yamamuro
Hiroki Segawa
Yuki Okada
Yuko T. Iwata
Publikationsdatum: 15.04.2023
Verlag: Springer Nature Singapore
Erschienen in: Forensic Toxicology / Ausgabe 2/2023
Print ISSN: 1860-8965
Elektronische ISSN: 1860-8973
DOI: https://doi.org/10.1007/s11419-023-00663-z

Detection of lisdexamfetamine and its metabolite d-amphetamine in urine and gastric contents collected from a cadaver at forensic autopsy

Open Access
Case Report

Δ9-Tetrahydrocannabinol stimulation of estrogen receptor-positive MCF-7 breast cancer cell migration: Interfering interaction with the estrogenic milieu

Short Communication

Emerging trends in methaqualone and analogues abuse: insights from online forums

Letter to the Editor

Cannabidiol in urine is not a proof of CBD consumption—lesson learned from urine sample analysis in routine caseworks

Original Article

Analysis of degradation products of Novichok agents in human urine by hydrophilic interaction liquid chromatography–tandem mass spectrometry

Open Access
Original Article

Liquid chromatography with tandem mass spectrometric method for determination of 425 drugs and poisons in dried blood spots and application to forensic cases

Original Article

Neu im Fachgebiet Rechtsmedizin

Pathologie der Milz

Hämatologische Neoplasien
CME

Nach einer Beschreibung der Milzanatomie und Darstellung der diagnostisch wichtigsten immunhistochemischen Färbungen zur Identifizierung der normalen Milzkompartimente werden am Beispiel eines nordafrikanischen Patienten mit rezentem …

Molekular definierte Nierenzellkarzinome 2025

Nierenkarzinom
Leitthema

Im Zuge der Überarbeitung der WHO-Klassifikation im Jahr 2022 konnten für mehrere Nierenzellkarzinome (NZK), die sich zuvor nicht eindeutig den bis dahin definierten Tumortypen zuordnen ließen, jedoch gemeinsame morphologische und molekulare …

Wichtige Änderungen in der WHO-Klassifikation der Hodentumoren 2022

Keimzelltumor
Leitthema

In der 5. Auflage der „WHO-Klassifikation der Tumoren der ableitenden Harnwege und des männlichen Genitaltrakts“ sind bedeutende Anpassungen an den bisherigen Klassifikationen vorgenommen worden. Diese betreffen bei den Keimzelltumoren des Hodens …

DNA mixture deconvolution using fully continuous models EuroForMix and EFMrep

Open Access
Originalien

Mixture deconvolution is a powerful tool for inferring individual DNA profiles from DNA mixtures for subsequent transmission to a database or database queries. To carry out deconvolution, a mixed trace can be interpreted either manually by an …

Springer Medizin

Evaluation of applicability of micro-segmental analysis to hair treated with heat and haircare products