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03.04.2020 | Original Investigation

Evaluation of Salmonella Typhi antigen YncE alongside HlyE for the detection of typhoid fever and its carriers

verfasst von: Freddy Franklin, Chun Wie Chong, Leong Huat Chua, Amy Amilda Anthony, Mervyn W. O. Liew, Ismail Aziah, Eugene Boon Beng Ong

Erschienen in: Medical Microbiology and Immunology | Ausgabe 5/2020

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Abstract

Typhoid fever is a disease caused by Salmonella Typhi that was implicated in millions of illnesses worldwide annually. Individuals that do not recover fully from typhoid fever can become asymptomatic carriers of the disease. Host antibodies against the S. Typhi antigens, HlyE (for acute typhoid) and YncE (for carriers) were previously reported to be useful biomarkers for the disease. Here, we expressed and purified recombinant HlyE and YncE antigens and tested the IgG, IgA and IgM responses in 422 sera samples retrieved from acute typhoid patients, other febrile, food handlers, and healthy individuals. The results showed that HlyE-IgG, -IgA and -IgM ELISAs have a collective sensitivity of 83% while YncE-IgG and -IgA ELISAs identified 16 possible carriers based on their antibody profiles. The identification of sensitive biomarkers for typhoid carrier detection is crucial for disease eradication.

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Titel: Evaluation of Salmonella Typhi antigen YncE alongside HlyE for the detection of typhoid fever and its carriers
verfasst von: Freddy Franklin
Chun Wie Chong
Leong Huat Chua
Amy Amilda Anthony
Mervyn W. O. Liew
Ismail Aziah
Eugene Boon Beng Ong
Publikationsdatum: 03.04.2020
Verlag: Springer Berlin Heidelberg
Erschienen in: Medical Microbiology and Immunology / Ausgabe 5/2020
Print ISSN: 0300-8584
Elektronische ISSN: 1432-1831
DOI: https://doi.org/10.1007/s00430-020-00667-1

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