Expression of CD180 in common B cell neoplasms using immunohistochemistry

Diagnosis of low-grade B cell lymphoma can be challenging especially on limited cytological and histological samples, CD180 is Toll-like receptor that was found to be of value in the diagnosis of marginal zone lymphoma using flow cytometry. In this pilot study, we examine the expression of CD180, using an immunohistochemical method, on a number of common B cell lymphoproliferative neoplasms. Immunohistochemical staining for sixty-seven cases of five B cell neoplasms, specifically, marginal zone lymphoma, mantle cell lymphoma, diffuse large B cell lymphoma, follicular lymphoma, and chronic lymphocytic leukemia/small lymphocytic lymphoma was performed on archived, paraffin-embedded, formalin-fixed tissue. CD180 expression is preserved in all cases of marginal zone lymphoma, sixteen out of 17 cases of follicular lymphoma cases, and none of the diffuse large B cell lymphoma or chronic lymphocytic leukemia/small lymphocytic lymphoma cases. One in 12 cases of mantle cell lymphoma shows weak and focal staining. Using CD180 immunohistochemical stain can be valuable and offer some help in the diagnosis of B cell neoplasms, especially on small tissue samples.