nach oben

International Urology and Nephrology

Erschienen in:

01.03.2009 | Urology-Original Paper

Expression profile and prognostic importance in prostate lesions of the reverse transcriptase component of human telomerase (hTERT) and of cyclin-dependent kinase inhibitor p57 (p57^kip2a)

verfasst von: Pınar Atasoy, Erdal Yilmaz, Onder Bozdogan, Sebnem Ayva, Ertan Batislam

Erschienen in: International Urology and Nephrology | Ausgabe 1/2009

Einloggen, um Zugang zu erhalten

Abstract

Objectives

To investigate expression of the reverse transcriptase component of human telomerase (hTERT) and of cyclin-dependent kinase inhibitor p57 (p57^kip2a) in prostate neoplasms and evaluate the relationship between these proteins and the Gleason score.

Methods

hTERT and p57^kip2a antibodies were studied by immunohistochemical methods in 70 prostate adenocarcinomas (33 high-grade and 37 low-grade carcinomas), 29 benign prostate hyperplasias, and 19 prostatic intraepithelial neoplasias (PIN). Only nuclear staining was evaluated with p57^kip2a whereas both nuclear and nucleolar staining were evaluated with hTERT.

Results

Immunohistochemical histologic scores (HSCOREs) of hTERT were significantly higher in the PIN group than in the hyperplasia group (P = 0.03). hTERT HSCOREs were not significantly different between hyperplasias and carcinomas or between low and high-grade carcinomas. p57^kip2a HSCOREs were significantly higher in hyperplasias than other groups, and in PINS than carcinomas, but did not differ significantly between low and high-grade carcinomas. A significant negative correlation was observed between hTERT and p57^kip2a (P = 0.007) in the hyperplasia group. No such correlation was found in PINs and carcinomas.

Conclusions

This study suggests that p57^kip2a is down-regulated in the malignant side of the spectrum of prostate carcinogenesis. Loss of p57^kip2a control on hTERT might have an important role in the development of prostate cancer.

Yano Y, Yoshida K, Osaki A et al (2002) Expression and distribution of human telomerase catalytic component, HTERT, in human breast tissue. Anticancer Res 22:4101–4107PubMed

Kanaya T, Kyo S, Hamada K et al (2000) Adenoviral expression of p53 represses telomerase activity through down-regulation of human telomerase reverse transcriptase transcription. Clin Cancer Res 6:1239–1247PubMed

Sharma GG, Gupta A, Wang H et al (2003) hTERT associates with human telomeres and enhances genomic stability and DNA repair. Oncogene 22:131–146PubMedCrossRef

Nijjar T, Wigington D, Garbe JC, Waha A, Stampfer MR, Yaswen P (1999) p57^kip27 Expression and loss of heterozygosity during immortal conversion of cultured human mammary epithelial cells. Cancer Res 59:5112–5118PubMed

Stampfer MR, Garbe J, Nijjar T et al (2003) Loss of p53 function accelerates acquisition of telomerase activity indefinite lifespan human mammary epithelial cell lines. Oncogene 22:5238–5251PubMedCrossRef

Lodygin D, Diebold J, Hermeking H (2004) Prostate cancer is characterized by epigenetic silencing of 14-3-3r expression. Oncogene 23:9034–9041PubMedCrossRef

Epstein JI, Wojno KJ (1999) The prostate and seminal vesicles. In: Sternberg SS (ed) Diagnostic surgical pathology. Lippincott Williams & Wilkins, Philedelphia, pp 1893–1942

Epstein JI, Herawi M (2006) Prostate needle biopsies containing prostatic intraepithelial neoplasia and atypical foci suspicious for carcinoma: implications for patient care. J Urol 175:820–834PubMedCrossRef

Nyholm HCJ, Nielsen AL, Lyndrup J, Dreisler A, Thorpe SM (1993) Estrogen and progesterone receptors in endometrial carcinoma: comparison of immunohistochemical and biochemical analysis. Int J Gynecol Pathol 12:246–252PubMedCrossRef

10.

Jemal A, Siegel R, Ward E et al (2007) Cancer Statistics. CA Cancer Clin 57:43–66CrossRef

11.

Latil A, Vidaud D, Valeri A et al (2000) hTERT expression correlates with in human prostate cancer. Int J Cancer 89:172–176PubMedCrossRef

12.

Zhang W, Kapusta LR, Slingerland JM, Klotz LH (1998) Telomerase activity in prostate cancer, prostatic intraepithelial neoplasia, and benign prostatic epithelium. Cancer Res 58:619–621PubMed

13.

Orlando C, Gelmini S, Selli C, Pazzagli M (2001) Telomerase in urological malignancy. J Urol 166:630–636CrossRef

14.

Bettendorf O, Heine B, Kneif S et al (2003) Expression-patterns of the RNA component (hTR) and the catalytic subunit (hTERT) of human telomerase in non-neoplastic prostate tissue, prostatic intraepithelial neoplasia, and prostate cancer. Prostate 55:99–104PubMedCrossRef

15.

Suenaga M, Soda H, Oka M et al (2002) Histone deacetylase inhibitors suppress telomerase reverse transcriptase mRNA expression in prostate cancer cells. Int J Cancer 97:621–625PubMedCrossRef

16.

Gil J, Kerai P, Lleonart M et al (2005) Immortalization of primary human prostate epithelial cells by c-myc. Cancer Res 65:2179–2185PubMedCrossRef

17.

Gonzalgo ML, Pavlovich CP, Lee SM, Nelson WG (2003) Prostate cancer detection by GSTP1 methylation analysis of postbiopsy urine specimens. Clin Cancer Res 9:2673–2677PubMed

18.

Tsihlias J, Kapusta LR, DeBoer G et al (1998) Loss of cyclin-dependent kinase inhibitor p27Kip1 is a novel prognostic factor in localized human prostate adenocarcinoma. Cancer Res 58:542–548PubMed

19.

Schwarze SR, Shi Y, Fu VX, Watson PA, Jarrard DF (2001) Role of cyclin-dependent kinase inhibitors in the growth arrest at senescence in human prostate epithelial and uroepithelial cells. Oncogene 20:8184–8192PubMedCrossRef

20.

Tahara H, Yasui W, Tahara E et al (1999) Immuno-histochemical detection of human telomerase catalytic component, hTERT, in human colorectal tumor and non-tumor tissue sections. Oncogene 18:1561–1567PubMedCrossRef

21.

Sabah M, Cummins R, Leader M, Kay M (2004) Expression of human telomerase reverse transcriptase in gastrointestinal stromal tumors occurs preferentially in malignant neoplasms. Hum Pathol 35:1231–1235PubMedCrossRef

22.

Iczkowski KA, Pantazis CG, McGregor DH, Wu Y, Tawfik OW (2002) Telomerase reverse transcriptase subunit immunoreactivity. Cancer 95:2487–2493PubMedCrossRef

23.

Pfitzenmaier J, Ellis WJ, Arfman EW (2006) Telomerase activity in disseminated prostate cancer cells. BJU Int 97:1309–1313PubMedCrossRef

24.

Nan KJ, Guo H, Ruan ZP, Jing Z, Liu SX (2005) Expression of p57^kip2 and its relationship with clinicopathology, PCNA and p53 in primary hepatocellular carcinoma. World J Gastroenterol 11:1237–1240PubMed

25.

Yue H, Jiang HY (2005) Expression of cell cycle regulator p57^kip2, cyclin E protein and proliferating cell nuclear antigen in human pancreatic cancer: an immunohistochemical study. World J Gastroenterol 11:5057–5060PubMed

Titel: Expression profile and prognostic importance in prostate lesions of the reverse transcriptase component of human telomerase (hTERT) and of cyclin-dependent kinase inhibitor p57 (p57kip2a)
verfasst von: Pınar Atasoy
Erdal Yilmaz
Onder Bozdogan
Sebnem Ayva
Ertan Batislam
Publikationsdatum: 01.03.2009
Verlag: Springer Netherlands
Erschienen in: International Urology and Nephrology / Ausgabe 1/2009
Print ISSN: 0301-1623
Elektronische ISSN: 1573-2584
DOI: https://doi.org/10.1007/s11255-008-9399-7

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

Reizdarmsyndrom: Diäten wirksamer als Medikamente

29.04.2024 Reizdarmsyndrom Nachrichten

Bei Reizdarmsyndrom scheinen Diäten, wie etwa die FODMAP-arme oder die kohlenhydratreduzierte Ernährung, effektiver als eine medikamentöse Therapie zu sein. Das hat eine Studie aus Schweden ergeben, die die drei Therapieoptionen im direkten Vergleich analysierte.

Notfall-TEP der Hüfte ist auch bei 90-Jährigen machbar

26.04.2024 Hüft-TEP Nachrichten

Ob bei einer Notfalloperation nach Schenkelhalsfraktur eine Hemiarthroplastik oder eine totale Endoprothese (TEP) eingebaut wird, sollte nicht allein vom Alter der Patientinnen und Patienten abhängen. Auch über 90-Jährige können von der TEP profitieren.

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

25.04.2024 Hypotonie Nachrichten

Wenn unter einer medikamentösen Hochdrucktherapie der diastolische Blutdruck in den Keller geht, steigt das Risiko für schwere kardiovaskuläre Ereignisse: Darauf deutet eine Sekundäranalyse der SPRINT-Studie hin.

Bei schweren Reaktionen auf Insektenstiche empfiehlt sich eine spezifische Immuntherapie

25.04.2024 Allergien und Intoleranzreaktionen Nachrichten

Insektenstiche sind bei Erwachsenen die häufigsten Auslöser einer Anaphylaxie. Einen wirksamen Schutz vor schweren anaphylaktischen Reaktionen bietet die allergenspezifische Immuntherapie. Jedoch kommt sie noch viel zu selten zum Einsatz.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Abstract

Objectives

Methods

Results

Conclusions

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 1/2009

Health-related quality of life after hand-assisted laparoscopic and open radical nephrectomies of renal cell carcinoma

The effect of mycophenolate mofetil on primary and secondary treatment of primary glomerulonephritis and lupus nephritis

Quality of life of neurogenic patients: translation and validation of the Portuguese version of Qualiveen

Use of genetic immunization to generate a high-level antibody against rat dicarboxylate transporter

Renal hilar extramedullary hematopoiesis presenting as incidental unilateral renal hilar mass in adolescent male: a case report

Hematospermia: etiological and management considerations